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Q58813

- FUCA_METJA

UniProt

Q58813 - FUCA_METJA

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Protein

L-fuculose phosphate aldolase

Gene

fucA

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reversible aldol cleavage of L-fuculose-1-phosphate to dihydroxyacetone phosphate (DHAP) and L-lactaldehyde. The substrate preference order is glyceraldehyde > DL-lactaldehyde, glycolaldehyde > acrolein > formaldehyde, methylglyoxal > acetaldehyde > crotonaldehyde. No activity was observed towards propionaldehyde.1 Publication

Catalytic activityi

L-fuculose 1-phosphate = glycerone phosphate + (S)-lactaldehyde.1 Publication

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Kineticsi

    Vmax=570 nmol/min/mg enzyme with glyceraldehyde as substrate (at pH 7.4 and at 70 degrees Celsius)1 Publication

    Vmax=501 nmol/min/mg enzyme with DL-lactaldehyde as substrate (at pH 7.4 and at 70 degrees Celsius)1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi68 – 681ZincBy similarity
    Metal bindingi87 – 871ZincBy similarity
    Metal bindingi89 – 891ZincBy similarity
    Metal bindingi147 – 1471ZincBy similarity

    GO - Molecular functioni

    1. L-fuculose-phosphate aldolase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fucose catabolic process Source: UniProtKB-UniPathway
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12176.
    UniPathwayiUPA00071.
    UPA00563; UER00626.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-fuculose phosphate aldolase (EC:4.1.2.17)
    Alternative name(s):
    L-fuculose-1-phosphate aldolase
    Gene namesi
    Name:fucA
    Ordered Locus Names:MJ1418
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 181181L-fuculose phosphate aldolasePRO_0000162932Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi243232.MJ1418.

    Structurei

    3D structure databases

    ProteinModelPortaliQ58813.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0235.
    InParanoidiQ58813.
    KOiK01628.
    OMAiEANGIVH.
    PhylomeDBiQ58813.

    Family and domain databases

    Gene3Di3.40.225.10. 1 hit.
    InterProiIPR001303. Aldolase_II/adducin_N.
    [Graphical view]
    PfamiPF00596. Aldolase_II. 1 hit.
    [Graphical view]
    SMARTiSM01007. Aldolase_II. 1 hit.
    [Graphical view]
    SUPFAMiSSF53639. SSF53639. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q58813-1 [UniParc]FASTAAdd to Basket

    « Hide

            10         20         30         40         50
    MDKKQFIKIC RKLYDRKYVV GSGGNVSVKE GDKIYLTPTG SILGFLKEDD
    60 70 80 90 100
    IAEMDLDGNV IKGKPTSEKN LHLMIYRKRN DINAIIHTHS LISTFLSTIN
    110 120 130 140 150
    KEIELLTPEG KIFLKKIGYV DYYEAGSLKL AEETAKRDED VIILKNHGVV
    160 170 180
    CLGKDLIDAY IKVEVLEEQA KLTLLNLLVK K
    Length:181
    Mass (Da):20,470
    Last modified:November 1, 1996 - v1
    Checksum:iE5F3BF13722145B0
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L77117 Genomic DNA. Translation: AAB99428.1.
    PIRiA64477.
    RefSeqiNP_248422.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB99428; AAB99428; MJ_1418.
    GeneIDi1452322.
    KEGGimja:MJ_1418.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L77117 Genomic DNA. Translation: AAB99428.1 .
    PIRi A64477.
    RefSeqi NP_248422.1. NC_000909.1.

    3D structure databases

    ProteinModelPortali Q58813.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ1418.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB99428 ; AAB99428 ; MJ_1418 .
    GeneIDi 1452322.
    KEGGi mja:MJ_1418.

    Phylogenomic databases

    eggNOGi COG0235.
    InParanoidi Q58813.
    KOi K01628.
    OMAi EANGIVH.
    PhylomeDBi Q58813.

    Enzyme and pathway databases

    UniPathwayi UPA00071 .
    UPA00563 ; UER00626 .
    BioCyci MetaCyc:MONOMER-12176.

    Family and domain databases

    Gene3Di 3.40.225.10. 1 hit.
    InterProi IPR001303. Aldolase_II/adducin_N.
    [Graphical view ]
    Pfami PF00596. Aldolase_II. 1 hit.
    [Graphical view ]
    SMARTi SM01007. Aldolase_II. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53639. SSF53639. 1 hit.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
    2. "Identification of lactaldehyde dehydrogenase in Methanocaldococcus jannaschii and its involvement in production of lactate for F420 biosynthesis."
      Grochowski L.L., Xu H., White R.H.
      J. Bacteriol. 188:2836-2844(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN F420 BIOSYNTHESIS, CATALYTIC ACTIVITY, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiFUCA_METJA
    AccessioniPrimary (citable) accession number: Q58813
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: November 26, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3