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Q58813

- FUCA_METJA

UniProt

Q58813 - FUCA_METJA

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Protein

L-fuculose phosphate aldolase

Gene
fucA, MJ1418
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reversible aldol cleavage of L-fuculose-1-phosphate to dihydroxyacetone phosphate (DHAP) and L-lactaldehyde. The substrate preference order is glyceraldehyde > DL-lactaldehyde, glycolaldehyde > acrolein > formaldehyde, methylglyoxal > acetaldehyde > crotonaldehyde. No activity was observed towards propionaldehyde.1 Publication

Catalytic activityi

L-fuculose 1-phosphate = glycerone phosphate + (S)-lactaldehyde.1 Publication

Cofactori

Binds 1 zinc ion per subunit Reviewed prediction.

Kineticsi

    Vmax=570 nmol/min/mg enzyme with glyceraldehyde as substrate (at pH 7.4 and at 70 degrees Celsius)1 Publication

    Vmax=501 nmol/min/mg enzyme with DL-lactaldehyde as substrate (at pH 7.4 and at 70 degrees Celsius)

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi68 – 681Zinc By similarity
    Metal bindingi87 – 871Zinc By similarity
    Metal bindingi89 – 891Zinc By similarity
    Metal bindingi147 – 1471Zinc By similarity

    GO - Molecular functioni

    1. L-fuculose-phosphate aldolase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fucose catabolic process Source: UniProtKB-UniPathway
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12176.
    UniPathwayiUPA00071.
    UPA00563; UER00626.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-fuculose phosphate aldolase (EC:4.1.2.17)
    Alternative name(s):
    L-fuculose-1-phosphate aldolase
    Gene namesi
    Name:fucA
    Ordered Locus Names:MJ1418
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 181181L-fuculose phosphate aldolasePRO_0000162932Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi243232.MJ1418.

    Structurei

    3D structure databases

    ProteinModelPortaliQ58813.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0235.
    KOiK01628.
    OMAiEANGIVH.
    PhylomeDBiQ58813.

    Family and domain databases

    Gene3Di3.40.225.10. 1 hit.
    InterProiIPR001303. Aldolase_II/adducin_N.
    [Graphical view]
    PfamiPF00596. Aldolase_II. 1 hit.
    [Graphical view]
    SMARTiSM01007. Aldolase_II. 1 hit.
    [Graphical view]
    SUPFAMiSSF53639. SSF53639. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q58813-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDKKQFIKIC RKLYDRKYVV GSGGNVSVKE GDKIYLTPTG SILGFLKEDD    50
    IAEMDLDGNV IKGKPTSEKN LHLMIYRKRN DINAIIHTHS LISTFLSTIN 100
    KEIELLTPEG KIFLKKIGYV DYYEAGSLKL AEETAKRDED VIILKNHGVV 150
    CLGKDLIDAY IKVEVLEEQA KLTLLNLLVK K 181
    Length:181
    Mass (Da):20,470
    Last modified:November 1, 1996 - v1
    Checksum:iE5F3BF13722145B0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99428.1.
    PIRiA64477.
    RefSeqiNP_248422.1. NC_000909.1.
    WP_010870936.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB99428; AAB99428; MJ_1418.
    GeneIDi1452322.
    KEGGimja:MJ_1418.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99428.1 .
    PIRi A64477.
    RefSeqi NP_248422.1. NC_000909.1.
    WP_010870936.1. NC_000909.1.

    3D structure databases

    ProteinModelPortali Q58813.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ1418.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB99428 ; AAB99428 ; MJ_1418 .
    GeneIDi 1452322.
    KEGGi mja:MJ_1418.

    Phylogenomic databases

    eggNOGi COG0235.
    KOi K01628.
    OMAi EANGIVH.
    PhylomeDBi Q58813.

    Enzyme and pathway databases

    UniPathwayi UPA00071 .
    UPA00563 ; UER00626 .
    BioCyci MetaCyc:MONOMER-12176.

    Family and domain databases

    Gene3Di 3.40.225.10. 1 hit.
    InterProi IPR001303. Aldolase_II/adducin_N.
    [Graphical view ]
    Pfami PF00596. Aldolase_II. 1 hit.
    [Graphical view ]
    SMARTi SM01007. Aldolase_II. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53639. SSF53639. 1 hit.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
    2. "Identification of lactaldehyde dehydrogenase in Methanocaldococcus jannaschii and its involvement in production of lactate for F420 biosynthesis."
      Grochowski L.L., Xu H., White R.H.
      J. Bacteriol. 188:2836-2844(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN F420 BIOSYNTHESIS, CATALYTIC ACTIVITY, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiFUCA_METJA
    AccessioniPrimary (citable) accession number: Q58813
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: September 3, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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