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Protein

Lactaldehyde dehydrogenase

Gene

MJ1411

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in F420 biosynthesis through the oxidation of lactaldehyde to lactate. The substrate preference order is propionaldehyde > DL-lactaldehyde, DL-glyceraldehyde > crotonaldehyde > glycolaldehyde > acetaldehyde, acrolein > formaldehyde. No activity was observed towards methylglyoxal or glyceraldehyde-3-phosphate. Has a preference for NAD over NADP.1 Publication

Catalytic activityi

(S)-lactaldehyde + NAD+ + H2O = (S)-lactate + NADH.1 Publication

Kineticsi

As the lactaldehyde used was a mixture of D and L isomers, the kinetic parameters do not reflect the possible stereospecificity of the enzyme.
  1. KM=284 µM for propionaldehyde (at pH 7.5)1 Publication
  2. KM=302 µM for DL-lactaldehyde (at pH 7.5)1 Publication
  1. Vmax=1.12 µmol/min/mg enzyme with propionaldehyde as substrate (at pH 7.5)1 Publication
  2. Vmax=0.6 µmol/min/mg enzyme with DL-lactaldehyde as substrate (at pH 7.5)1 Publication

pH dependencei

Optimum pH is 9. The activity remained constant to pH 10.0. Below pH 9, the activity gradually declined, and the enzyme was inactive at pH 6 or below.1 Publication

Pathwayi: coenzyme F420 biosynthesis

This protein is involved in the pathway coenzyme F420 biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway coenzyme F420 biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei240By similarity1
Active sitei274By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi220 – 225NADBy similarity6

GO - Molecular functioni

  • lactaldehyde dehydrogenase activity Source: MENGO

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12177.
BRENDAi1.2.1.22. 3260.
SABIO-RKiQ58806.
UniPathwayiUPA00071.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactaldehyde dehydrogenase (EC:1.2.1.22)
Gene namesi
Ordered Locus Names:MJ1411
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000566001 – 463Lactaldehyde dehydrogenaseAdd BLAST463

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi243232.MJ_1411.

Structurei

Secondary structure

1463
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 16Combined sources4
Turni18 – 20Combined sources3
Beta strandi23 – 27Combined sources5
Helixi32 – 44Combined sources13
Helixi46 – 50Combined sources5
Helixi54 – 70Combined sources17
Helixi72 – 83Combined sources12
Helixi87 – 110Combined sources24
Beta strandi113 – 116Combined sources4
Beta strandi121 – 129Combined sources9
Beta strandi131 – 137Combined sources7
Beta strandi139 – 141Combined sources3
Helixi142 – 155Combined sources14
Beta strandi159 – 164Combined sources6
Helixi170 – 185Combined sources16
Helixi190 – 192Combined sources3
Beta strandi193 – 195Combined sources3
Turni200 – 202Combined sources3
Helixi203 – 209Combined sources7
Beta strandi215 – 220Combined sources6
Helixi222 – 231Combined sources10
Beta strandi234 – 240Combined sources7
Beta strandi245 – 249Combined sources5
Helixi255 – 267Combined sources13
Helixi268 – 271Combined sources4
Beta strandi276 – 283Combined sources8
Helixi284 – 286Combined sources3
Helixi287 – 299Combined sources13
Helixi319 – 334Combined sources16
Beta strandi338 – 341Combined sources4
Beta strandi353 – 356Combined sources4
Helixi362 – 364Combined sources3
Beta strandi370 – 378Combined sources9
Helixi380 – 387Combined sources8
Beta strandi394 – 399Combined sources6
Helixi403 – 412Combined sources10
Beta strandi415 – 422Combined sources8
Helixi437 – 439Combined sources3
Beta strandi440 – 442Combined sources3
Helixi446 – 452Combined sources7
Beta strandi454 – 462Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PQAX-ray1.50A/B/C/D1-463[»]
3RHDX-ray2.20A/B/C/D1-463[»]
ProteinModelPortaliQ58806.
SMRiQ58806.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiarCOG01252. Archaea.
COG1012. LUCA.
InParanoidiQ58806.
KOiK19266.
OMAiYEFFTEV.
OrthoDBiPOG093Z01H3.
PhylomeDBiQ58806.

Family and domain databases

Gene3Di3.40.605.10. 1 hit.
InterProiView protein in InterPro
IPR016161. Ald_DH/histidinol_DH.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
PfamiView protein in Pfam
PF00171. Aldedh. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.

Sequencei

Sequence statusi: Complete.

Q58806-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFIDGKWINR EDMDVINPYS LEVIKKIPAL SREEAKEAID TAEKYKEVMK
60 70 80 90 100
NLPITKRYNI LMNIAKQIKE KKEELAKILA IDAGKPIKQA RVEVERSIGT
110 120 130 140 150
FKLAAFYVKE HRDEVIPSDD RLIFTRREPV GIVGAITPFN FPLNLSAHKI
160 170 180 190 200
APAIATGNVI VHHPSSKAPL VCIELAKIIE NALKKYNVPL GVYNLLTGAG
210 220 230 240 250
EVVGDEIVVN EKVNMISFTG SSKVGELITK KAGFKKIALE LGGVNPNIVL
260 270 280 290 300
KDADLNKAVN ALIKGSFIYA GQVCISVGMI LVDESIADKF IEMFVNKAKV
310 320 330 340 350
LNVGNPLDEK TDVGPLISVE HAEWVEKVVE KAIDEGGKLL LGGKRDKALF
360 370 380 390 400
YPTILEVDRD NILCKTETFA PVIPIIRTNE EEMIDIANST EYGLHSAIFT
410 420 430 440 450
NDINKSLKFA ENLEFGGVVI NDSSLFRQDN MPFGGVKKSG LGREGVKYAM
460
EEMSNIKTII ISK
Length:463
Mass (Da):51,136
Last modified:June 1, 1998 - v2
Checksum:i46F265686595B6A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99418.1.
PIRiB64476.
RefSeqiWP_010870928.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99418; AAB99418; MJ_1411.
GeneIDi1452314.
KEGGimja:MJ_1411.

Similar proteinsi

Entry informationi

Entry nameiLADH_METJA
AccessioniPrimary (citable) accession number: Q58806
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: June 1, 1998
Last modified: June 7, 2017
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families