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Protein

Putative ATP-dependent RNA helicase TDRD12

Gene

TDRD12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Probable ATP-binding RNA helicase required during spermatogenesis to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process. Acts via the PET complex, a multiprotein complex required during the secondary piRNAs metabolic process for the PIWIL2 slicing-triggered loading of PIWIL4 piRNAs.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi460 – 4678ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Helicase, Hydrolase

Keywords - Biological processi

Differentiation, Meiosis, RNA-mediated gene silencing, Spermatogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-5601884. PIWI-interacting RNA (piRNA) biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ATP-dependent RNA helicase TDRD12 (EC:3.6.4.13)
Alternative name(s):
ES cell-associated transcript 8 protein
Tudor domain-containing protein 12
Gene namesi
Name:TDRD12
Synonyms:ECAT8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:25044. TDRD12.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162405542.

Polymorphism and mutation databases

BioMutaiTDRD12.
DMDMi162416281.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11771177Putative ATP-dependent RNA helicase TDRD12PRO_0000311968Add
BLAST

Proteomic databases

EPDiQ587J7.
PaxDbiQ587J7.
PRIDEiQ587J7.

PTM databases

iPTMnetiQ587J7.
PhosphoSiteiQ587J7.

Expressioni

Gene expression databases

BgeeiQ587J7.
CleanExiHS_TDRD12.
ExpressionAtlasiQ587J7. baseline and differential.

Organism-specific databases

HPAiHPA042684.

Interactioni

Subunit structurei

Component of a mRNP complex containing PIWIL2, TDRD1 and piRNAs. Component of the PET complex, at least composed of EXD1, PIWIL2, TDRD12 and piRNAs.By similarity

Protein-protein interaction databases

IntActiQ587J7. 1 interaction.
MINTiMINT-8415333.
STRINGi9606.ENSP00000390621.

Structurei

3D structure databases

ProteinModelPortaliQ587J7.
SMRiQ587J7. Positions 413-636.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 11863Tudor 1Add
BLAST
Domaini447 – 635189Helicase ATP-bindingAdd
BLAST
Domaini900 – 999100Tudor 2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi574 – 5774DEAH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.Curated
Contains 2 Tudor domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0331. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00420000029847.
HOGENOMiHOG000154538.
HOVERGENiHBG108554.
InParanoidiQ587J7.
KOiK18409.
OMAiMCQDIEI.
OrthoDBiEOG7XSTD1.
PhylomeDBiQ587J7.
TreeFamiTF332354.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR027417. P-loop_NTPase.
IPR002999. Tudor.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00567. TUDOR. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q587J7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLQLLVLKIE DPGCFWVIIK GCSPFLDHDV DYQKLNSAMN DFYNSTCQDI
60 70 80 90 100
EIKPLTLEEG QVCVVYCEEL KCWCRAIVKS ITSSADQYLA ECFLVDFAKN
110 120 130 140 150
IPVKSKNIRV VVESFMQLPY RAKKFSLYCT KPVTLHIDFC RDSTDIVPAK
160 170 180 190 200
KWDNAAIQYF QNLLKATTQV EARLCAVEED TFEVYLYVTI KDEKVCVNDD
210 220 230 240 250
LVAKNYACYM SPTKNKNLDY LEKPRLNIKS APSFNKLNPA LTLWPMFLQG
260 270 280 290 300
KDVQGMEDSH GVNFPAQSLQ HTWCKGIVGD LRPTATAQDK AVKCNMDSLR
310 320 330 340 350
DSPKDKSEKK HHCISLKDTN KRVESSVYWP AKRGITIYAD PDVPEASALS
360 370 380 390 400
QKSNEKPLRL TEKKEYDEKN SCVKLLQFLN PDPLRADGIS DLQQLQKLKG
410 420 430 440 450
LQPPVVVLRN KIKPCLTIDS SPLSADLKKA LQRNKFPGPS HTESYSWPPI
460 470 480 490 500
ARGCDVVVIS HCESNPLLYL LPVLTVLQTG ACYKSLPSRN GPLAVIVCPG
510 520 530 540 550
WKKAQFIFEL LGEYSMSSRP LHPVLLTIGL HKEEAKNTKL PRGCDVIVTT
560 570 580 590 600
PYSLLRLLAC QSLLFLRLCH LILDEVEVLF LEANEQMFAI LDNFKKNIEV
610 620 630 640 650
EERESAPHQI VAVGVHWNKH IEHLIKEFMN DPYIVITAME EAALYGNVQQ
660 670 680 690 700
VVHLCLECEK TSSLLQALDF IPSQAQKTLI FTCSVAETEI VCKVVESSSI
710 720 730 740 750
FCLKMHKEMI FNLQNVLEQW KKKLSSGSQI ILALTDDCVP LLAITDATCV
760 770 780 790 800
IHFSFPASPK VFGGRLYCMS DHFHAEQGSP AEQGDKKAKS VLLLTEKDAS
810 820 830 840 850
HAVGVLRYLE RADAKVPAEL YEFTAGVLEA KEDKKAGRPL CPYLKAFGFC
860 870 880 890 900
KDKRICPDRH RINPETDLPR KLSSQALPSF GYIKIIPFYI LNATNYFGRI
910 920 930 940 950
VDKHMDLYAT LNAEMNEYFK DSNKTTVEKV EKFGLYGLAE KTLFHRVQVL
960 970 980 990 1000
EVNQKEDAWA LDDILVEFID EGRTGLVTRD QLLHLPEHFH TLPPQAVEFI
1010 1020 1030 1040 1050
VCRVKPADNE IEWNPKVTRY IHHKIVGKLH DAKVILALGN TVWIDPMVHI
1060 1070 1080 1090 1100
TNLSSLKTSV IDYNVRAEIL SMGMGIDNPE HIEQLKKLRE DAKIPACEES
1110 1120 1130 1140 1150
LSQTPPRVTG TSPAQDQDHP SEEQGGQGTP PAEDAACLQS PQPEDTGAEG
1160 1170
GAESKTSSEN QKPGGYLVFK RWLSSNR
Note: No experimental confirmation available.
Length:1,177
Mass (Da):132,578
Last modified:December 4, 2007 - v2
Checksum:i35C6C813A228132F
GO
Isoform 2 (identifier: Q587J7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     395-395: L → T
     396-1177: Missing.

Show »
Length:395
Mass (Da):45,033
Checksum:i7D93CB85DE5065E0
GO

Sequence cautioni

The sequence BAD95490.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81K → Q in BAD95490 (PubMed:12787504).Curated
Sequence conflicti107 – 1071N → S in BAD95490 (PubMed:12787504).Curated
Sequence conflicti413 – 4131K → E in BC049000 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei395 – 3951L → T in isoform 2. 1 PublicationVSP_029675
Alternative sequencei396 – 1177782Missing in isoform 2. 1 PublicationVSP_029676Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008736 Genomic DNA. No translation available.
AC008805 Genomic DNA. No translation available.
BC049000 mRNA. No translation available.
AB211063 mRNA. Translation: BAD95490.1. Different initiation.
CCDSiCCDS46038.1. [Q587J7-2]
RefSeqiNP_001104292.1. NM_001110822.1. [Q587J7-2]
UniGeneiHs.646351.
Hs.729739.

Genome annotation databases

EnsembliENST00000421545; ENSP00000390621; ENSG00000173809. [Q587J7-2]
ENST00000444215; ENSP00000416248; ENSG00000173809. [Q587J7-1]
GeneIDi91646.
KEGGihsa:91646.
UCSCiuc002ntq.3. human. [Q587J7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008736 Genomic DNA. No translation available.
AC008805 Genomic DNA. No translation available.
BC049000 mRNA. No translation available.
AB211063 mRNA. Translation: BAD95490.1. Different initiation.
CCDSiCCDS46038.1. [Q587J7-2]
RefSeqiNP_001104292.1. NM_001110822.1. [Q587J7-2]
UniGeneiHs.646351.
Hs.729739.

3D structure databases

ProteinModelPortaliQ587J7.
SMRiQ587J7. Positions 413-636.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ587J7. 1 interaction.
MINTiMINT-8415333.
STRINGi9606.ENSP00000390621.

PTM databases

iPTMnetiQ587J7.
PhosphoSiteiQ587J7.

Polymorphism and mutation databases

BioMutaiTDRD12.
DMDMi162416281.

Proteomic databases

EPDiQ587J7.
PaxDbiQ587J7.
PRIDEiQ587J7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000421545; ENSP00000390621; ENSG00000173809. [Q587J7-2]
ENST00000444215; ENSP00000416248; ENSG00000173809. [Q587J7-1]
GeneIDi91646.
KEGGihsa:91646.
UCSCiuc002ntq.3. human. [Q587J7-1]

Organism-specific databases

CTDi91646.
GeneCardsiTDRD12.
HGNCiHGNC:25044. TDRD12.
HPAiHPA042684.
neXtProtiNX_Q587J7.
PharmGKBiPA162405542.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0331. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00420000029847.
HOGENOMiHOG000154538.
HOVERGENiHBG108554.
InParanoidiQ587J7.
KOiK18409.
OMAiMCQDIEI.
OrthoDBiEOG7XSTD1.
PhylomeDBiQ587J7.
TreeFamiTF332354.

Enzyme and pathway databases

ReactomeiR-HSA-5601884. PIWI-interacting RNA (piRNA) biogenesis.

Miscellaneous databases

GenomeRNAii91646.
PROiQ587J7.

Gene expression databases

BgeeiQ587J7.
CleanExiHS_TDRD12.
ExpressionAtlasiQ587J7. baseline and differential.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR027417. P-loop_NTPase.
IPR002999. Tudor.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00567. TUDOR. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  3. "The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells."
    Mitsui K., Tokuzawa Y., Itoh H., Segawa K., Murakami M., Takahashi K., Maruyama M., Maeda M., Yamanaka S.
    Cell 113:631-642(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-1177 (ISOFORM 2).

Entry informationi

Entry nameiTDR12_HUMAN
AccessioniPrimary (citable) accession number: Q587J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 4, 2007
Last modified: June 8, 2016
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.