Reviewed, UniProtKB/Swiss-Prot Q58772 (SYE_METJA)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase
      Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	MJ1377

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subcellular location	Cytoplasm. HAMAP MF_00022
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 553	553	Glutamyl-tRNA synthetase HAMAP MF_00022		PRO_0000119717
Regions
	Motif	98 – 108	11	"HIGH" region HAMAP MF_00022

Sequences

	Sequence		Length	Mass (Da)
	Q58772-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B19D4177990454A0	FASTA	553	64,577
	10 20 30 40 50 60 MEEKILPIAL RNAIKYNGKA NPKAVLGIFL SENPEYRSKA KEVMPIVEKV VEEVNKLSLD 70 80 90 100 110 120 EIKKKLEELG EDVKKKEKKE KGLELPNVKD KVVMRFAPNP SGPLHIGHAR AAVLNDYFVK 130 140 150 160 170 180 KYGGKLILRL EDTDPKRVLP EAYDMIKEDL DWLGVKVDEV VIQSDRIELY YEYGRKLIEM 190 200 210 220 230 240 GHAYVCDCNP EEFRELRNKG VPCKCRDRAI EDNLELWEKM LNGELENVAV RLKTDIKHKN 250 260 270 280 290 300 PSIRDFPIFR VEKTPHPRTG DKYCVYPLMN FSVPVDDHLL GMTHVLRGKD HIVNTEKQAY 310 320 330 340 350 360 IYKYFGWEMP EFIHYGILKI EDIVLSTSSM YKGIKEGLYS GWDDVRLGTL RALRRRGIKP 370 380 390 400 410 420 EAIYEIMKRI GIKQADVKFS WENLYAINKE LIDKDARRFF FVWNPKKLII EGAEKKVLKL 430 440 450 460 470 480 RMHPDRPEFG ERELIFDGEV YVVGDELEEN KMYRLMELFN IVVEKVDDIA LAKYHSDDFK 490 500 510 520 530 540 IARKNKAKII HWIPVKDSVK VKVLMPDGEI KEGFAEKDFA KVEVDDIIQF ERFGFVRIDK 550 KDNDGFVCCY AHR

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB99384.1.
PIR	H64471.
RefSeq	NP_248380.1.
3D structure databases
HSSP	HSSP built from PDB template 1NYL based on UniProtKB P00962.
ModBase	Search...
Genome annotation databases
GeneID	1452280.
GenomeReviews	Gene locus MJ1377 in contig L77117_GR.
KEGG	mja:MJ1377.
NMPDR	fig|243232.1.peg.1417.
TIGR	MJ1377.
Phylogenomic databases
HOGENOM	Q58772.
OMA	MRFAPNP.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1377-MON.
BRENDA	6.1.1.17. 256362.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004526. Glu-tRNA-synth_Ic_arc/euk. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00463. gltX_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_METJA

Accession

Primary (citable) accession number: Q58772

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)