Q58761 (KPRS_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase
Short name=RPPK
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase
Short name=P-Rib-PP synthase
Short name=PRPP synthase

Gene names

Name:	prs
Ordered Locus Names:	MJ1366

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00583_A
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP-Rule MF_00583_A
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.
Sequence caution	The sequence AAB99374.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	5-phosphoribose 1-diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway nucleoside metabolic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 284

284

Ribose-phosphate pyrophosphokinase HAMAP-Rule MF_00583_A

PRO_0000141238

Regions

Region

205 – 218

14

Binding of phosphoribosylpyrophosphate Potential

Sites

Metal binding

123

1

Magnesium Potential

Metal binding

125

1

Magnesium Potential

Secondary structure

1

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284

Helix Strand Turn

Details...

Beta strand

2 – 5

4

Helix

7 – 9

3

Helix

10 – 19

10

Beta strand

27 – 31

5

Beta strand

37 – 41

5

Beta strand

47 – 53

7

Helix

59 – 74

16

Turn

75 – 77

3

Beta strand

80 – 84

5

Turn

89 – 92

4

Helix

104 – 115

12

Beta strand

117 – 123

7

Helix

127 – 132

6

Beta strand

137 – 140

4

Helix

143 – 150

8

Turn

151 – 153

3

Beta strand

158 – 163

6

Helix

164 – 166

3

Helix

167 – 177

11

Beta strand

181 – 184

4

Beta strand

198 – 200

3

Beta strand

208 – 214

7

Beta strand

216 – 218

3

Helix

219 – 230

12

Beta strand

235 – 242

8

Helix

249 – 256

8

Beta strand

259 – 264

6

Beta strand

272 – 274

3

Helix

277 – 281

5

Sequences

	Sequence		Length	Mass (Da)
	Q58761 [UniParc]. Last modified October 25, 2002. Version 2. Checksum: 9EAE9805785B1127	FASTA	284	31,395
	10 20 30 40 50 60 MIVVSGSQSQ NLAFKVAKLL NTKLTRVEYK RFPDNEIYVR IVDEINDDEA VIINTQKNQN 70 80 90 100 110 120 DAIVETILLC DALRDEGVKK ITLVAPYLAY ARQDKKFNPG EAISIRALAK IYSNIVDKLI 130 140 150 160 170 180 TINPHETHIK DFFTIPFIYG DAVPKLAEYV KDKLNDPIVL APDKGALEFA KTASKILNAE 190 200 210 220 230 240 YDYLEKTRLS PTEIQIAPKT LDAKDRDVFI VDDIISTGGT MATAVKLLKE QGAKKIIAAC 250 260 270 280 VHPVLIGDAL NKLYSAGVEE VVGTDTYLSE VSKVSVAEVI VDLL

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L77117 Genomic DNA. Translation: AAB99374.1. Different initiation.

PIR

E64470.

RefSeq

NP_248369.1. NC_000909.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1U9Y	X-ray	2.65	A/B/C/D	1-284	[»]
1U9Z	X-ray	2.80	A/B/C/D	1-284	[»]

ProteinModelPortal

Q58761.

SMR

Q58761. Positions 1-284.

ModBase

Search...

Protein-protein interaction databases

STRING

243232.MJ1366.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAB99374; AAB99374; MJ_1366.

GeneID

1452269.

KEGG

mja:MJ_1366.

Phylogenomic databases

eggNOG

COG0462.

KO

K00948.

OMA

MQANHPD.

ProtClustDB

PRK00934.

Enzyme and pathway databases

UniPathway

UPA00087; UER00172.

Family and domain databases

HAMAP

MF_00583_A. RibP_PPkinase_A.

InterPro

IPR000836. PRibTrfase_dom.
IPR005946. Rib-P_diPkinase.
[Graphical view]

Pfam

PF00156. Pribosyltran. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01251. ribP_PPkin. 1 hit.

PROSITE

PS00114. PRPP_SYNTHASE. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q58761.

Entry information

Entry name

KPRS_METJA

Accession

Primary (citable) accession number: Q58761

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	October 25, 2002
Last modified:	May 1, 2013
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program