Q58731 (GMHA_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable phosphoheptose isomerase
EC=5.3.1.28

Alternative name(s):
Sedoheptulose 7-phosphate isomerase

Gene names

Name:	gmhA
Ordered Locus Names:	MJ1335

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate By similarity. HAMAP MF_00067
Catalytic activity	D-sedoheptulose 7-phosphate = D-glycero-D-manno-heptose 7-phosphate. HAMAP MF_00067
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00067
Pathway	Carbohydrate biosynthesis; D-glycero-D-manno-heptose 7-phosphate biosynthesis; D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1. HAMAP MF_00067
Subcellular location	Cytoplasm By similarity HAMAP MF_00067.
Miscellaneous	The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate By similarity. HAMAP MF_00067
Sequence similarities	Belongs to the SIS family. GmhA subfamily. Contains 1 SIS domain.
Sequence caution	The sequence AAB99342.1 differs from that shown. Reason: Frameshift at position 49.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide core region biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	D-sedoheptulose 7-phosphate isomerase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 187	187	Probable phosphoheptose isomerase HAMAP MF_00067		PRO_0000136552
Regions
	Domain	32 – 187	156	SIS
	Region	47 – 49	3	Substrate binding By similarity
	Region	89 – 90	2	Substrate binding By similarity
	Region	115 – 117	3	Substrate binding By similarity
Sites
	Metal binding	56	1	Zinc By similarity
	Metal binding	60	1	Zinc By similarity
	Metal binding	167	1	Zinc By similarity
	Metal binding	175	1	Zinc By similarity
	Binding site	60	1	Substrate By similarity
	Binding site	120	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58731 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 38440D7520757DB4	FASTA	187	20,399
	10 20 30 40 50 60 MIMKKYFEES ANVKLKFIEE DEEKLKKAIE VIYNALKNGN KILICGNGGS AANSQHFAAE 70 80 90 100 110 120 IVGRFKLERK GLPAIALTTD ISILTAIGND YGFDRIFERQ VEALGKEGDV LAGISTSGNS 130 140 150 160 170 180 ENVIKAANKA KEMGIYTIGL LGKGGGKLKD IVDLALVVPS NDTARIQECH LTIYHVICEE VEKKLVK

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99342.1. Frameshift.
PIR	F64466.
RefSeq	NP_248337.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58731.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1452238.
GenomeReviews	Gene locus MJ1335 in contig L77117_GR.
KEGG	mja:MJ_1335.
TIGR	MJ1335.
Phylogenomic databases
HOGENOM	HBG671955.
OMA	HITIIHI.
Family and domain databases
HAMAP	MF_00067. GmhA. [Tree]
InterPro	IPR004515. Phosphoheptose_Isoase_subgr. IPR020620. Phosphoheptose_isomerase. IPR001347. SIS. [Graphical view]
KO	K03271.
TIGRFAMs	TIGR00441. GmhA. 1 hit.
PROSITE	PS51464. SIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GMHA_METJA

Accession

Primary (citable) accession number: Q58731

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	November 16, 2011
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program