Q58725 (AMPM_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase
Short name=MAP
EC=3.4.11.18

Alternative name(s):
Peptidase M

Gene names

Name:	map
Ordered Locus Names:	MJ1329

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Methionine aminopeptidase		PRO_0000148975
Sites
	Metal binding	85	1	Cobalt 1 By similarity
	Metal binding	96	1	Cobalt 1 By similarity
	Metal binding	96	1	Cobalt 2 By similarity
	Metal binding	156	1	Cobalt 2 By similarity
	Metal binding	189	1	Cobalt 2 By similarity
	Metal binding	279	1	Cobalt 1 By similarity
	Metal binding	279	1	Cobalt 2 By similarity
	Binding site	65	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58725 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: AA28D37E2BF34186	FASTA	294	32,950
	10 20 30 40 50 60 MEIEGYEKII EAGKIASKVR EEAVKLIXPG VKLLEVAEFV ENRIRELGGE PAFPCNISIN 70 80 90 100 110 120 EIAAHYTPKL NDNLEFKDDD VVKLDLGAHV DGYIADTAIT VDLSNSYKDL VKASEDALYT 130 140 150 160 170 180 VIKEINPPMN IGEMGKIIQE VIESYGYKPI SNLSGHVMHR YELHTGISIP NVYERTNQYI 190 200 210 220 230 240 DVGDLVAIEP FATDGFGMVK DGNLGNIYKF LAKRPIRLPQ ARKLLDVISK NYPYLPFAER 250 260 270 280 290 WVLKNESERL ALNSLIRASC IYGYPILKER ENGIVGQAEH TILITENGVE ITTK

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99338.1.
PIR	H64465.
RefSeq	NP_248330.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58725.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1452231.
GenomeReviews	Gene locus MJ1329 in contig L77117_GR.
KEGG	mja:MJ_1329.
NMPDR	fig|243232.1.peg.1367.
TIGR	MJ1329.
Phylogenomic databases
HOGENOM	HBG318153.
OMA	IEPFATN.
ProtClustDB	PRK08671.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1329-MONOMER.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002468. Pept_M24A_MAP2. IPR018349. Pept_M24A_MAP2_BS. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 2 hits. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K01265.
PANTHER	PTHR10804:SF9. Pept_M24A_MAP2. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00501. Met_pdase_II. 1 hit.
PROSITE	PS01202. MAP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_METJA

Accession

Primary (citable) accession number: Q58725

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 16, 2011
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program