Q58723 (T2M4_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme MjaIV
Short name=R.MjaIV
EC=3.1.21.4

Alternative name(s):
Endonuclease MjaIV
Type II restriction enzyme MjaIV

Gene names

Name:	mjaIVR
Ordered Locus Names:	MJ1327

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes the double-stranded unmethylated sequence GTNNAC.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Type-2 restriction enzyme MjaIV		PRO_0000077335

Sequences

	Sequence		Length	Mass (Da)
	Q58723 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4597C2B8EF1FA973	FASTA	245	28,779
	10 20 30 40 50 60 MVVKLVNNEL KILSGKLRDI FREIYNKIKN DENINDRNLD DKVLSLLKDY TISEENLKIK 70 80 90 100 110 120 FSEPKDEIYS YEGRRTPYDL LCYGIINGKN FLIFINNKFG DLKSNTRNDV TTYNNLLRLY 130 140 150 160 170 180 LGIKRQRLTS EITINGELVY NRISGNEIVS YGIFVVDKYR RGYKFFLLEE IKDDFYVNPR 190 200 210 220 230 240 NNMFQIRYSP NLGDPIDYFA FVKKLIDAIL ESLEKSLNSI KTEILVLNSI KIQLINIKEG KHGED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Method for screening restriction endonucleases." Noren C.J., Roberts R.J., Patti J., Byrd D.R., Morgan R.D. Patent number WO9911821, 11-MAR-1999 Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99343.1.
PIR	F64465.
RefSeq	NP_248327.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58723.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJ1327.
Protein family/group databases
REBASE	3894. MjaIV.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB99343; AAB99343; MJ_1327.
GeneID	1452229.
KEGG	mja:MJ_1327.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2M4_METJA

Accession

Primary (citable) accession number: Q58723

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program