Reviewed, UniProtKB/Swiss-Prot Q58711 (PRNK_METJA)

Names and origin

Protein names

Recommended name:
    Polyribonucleotide 5'-hydroxyl-kinase MJ1315
    EC=2.7.1.78
Alternative name(s):
    Polynucleotide kinase MJ1315

Gene names

Ordered Locus Names:

MJ1315

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of both single-stranded RNA (ssRNA) and single-stranded DNA (ssDNA). Exhibits a strong preference for ssRNA By similarity.
Catalytic activity	ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA. ATP + 5'-dephospho-RNA = ADP + 5'-phospho-RNA.
Cofactor	Divalent cation By similarity.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Polyribonucleotide 5'-hydroxyl-kinase MJ1315		PRO_0000107272
Regions
	Nucleotide binding	39 – 46	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q58711-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E2397969BE7E8870	FASTA	361	41,352
	10 20 30 40 50 60 MVDNMISKAY YTTEIPEDRF EALSCIKDSQ KPLKIILLGG VDSGKTTLAT FLANELLNLG 70 80 90 100 110 120 FKVAIVDSDV GQKSILPPAT ISLAFPETNF NNLYEIKPYK SYFVGSTAPI QFFGEMITGT 130 140 150 160 170 180 KLLCDYAEDK ADIIIVDTTG LISGSGADLK RMKIEMIKPD IIIALEKRNE LKSILKPFEN 190 200 210 220 230 240 KIRVFYLKVY ENAKSFSREE RKEIRAEKWK EYFKNSKIYN IGFNDVVIGG TKVFQGEKIL 250 260 270 280 290 300 EDEKYLLESL FKWKILYGSK CDGRYTIVKR DLVNMPRQID KNILYYIEPE RFNNLIVGLI 310 320 330 340 350 360 DEDSFCIGLG ILKTIDFENE TLEILTPISE EDIKNIREIR FGRIRVDENG EELGLLDRDS I

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB99322.1.
PIR	B64464.
RefSeq	NP_248315.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1452217.
GenomeReviews	Gene locus MJ1315 in contig L77117_GR.
KEGG	mja:MJ1315.
NMPDR	fig|243232.1.peg.1352.
TIGR	MJ1315.
Phylogenomic databases
HOGENOM	Q58711.
OMA	NDLGPPT.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1315-MON.
Family and domain databases
InterPro	IPR012581. NUC156. [Graphical view]
Pfam	PF08160. NUC156. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRNK_METJA

Accession

Primary (citable) accession number: Q58711

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)