Q58694 (BIOF_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative 8-amino-7-oxononanoate synthase
Short name=AONS
EC=2.3.1.47

Alternative name(s):
7-keto-8-amino-pelargonic acid synthase
Short name=7-KAP synthase
8-amino-7-ketopelargonate synthase

Gene names

Name:	bioF
Ordered Locus Names:	MJ1298

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide By similarity.
Catalytic activity	Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein].
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	8-amino-7-oxononanoate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Putative 8-amino-7-oxononanoate synthase		PRO_0000163820
Regions
	Region	94 – 95	2	Pyridoxal phosphate binding By similarity
	Region	192 – 195	4	Pyridoxal phosphate binding By similarity
	Region	223 – 226	4	Pyridoxal phosphate binding By similarity
Sites
	Binding site	20	1	Substrate By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	167	1	Pyridoxal phosphate By similarity
	Binding site	337	1	Substrate By similarity
Amino acid modifications
	Modified residue	226	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58694 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 145B2FA8A5F8B9D5	FASTA	372	41,895
	10 20 30 40 50 60 MFREKLRREI EIIKNNGLYR FLRKKDDGVL DFSSNDYLCL SKHPEVIEAV KEGLKYGAGS 70 80 90 100 110 120 TGSRLTSGNI NHQRLEEKIA EFKETERTLV YSSGYATNVG VISALCKKGD LILSDKLNHA 130 140 150 160 170 180 SIIDGCKLSK ADVLIYNHCD VEHLTNLIEE NWGKYNNLFI VTDGVFSMDG DIAPLRDLKK 190 200 210 220 230 240 IADEFNAILI IDDAHGTGVL GDGRGTLKHF NLKPSDNIVQ IGTLSKAIGG LGGFVCGIEE 250 260 270 280 290 300 VVEYLINTSR SFIFSTALPP HVVEGCIKAF EIIEKTDIVK KLQKNIKIAN KVFKKYEFIK 310 320 330 340 350 360 EDNLTPIYPF IFKEKTMEIA EHLIKNNIFC VGIRYPTVPK GLERIRVSIN VGHEKEDFEL 370 LCERIKEVYS SD

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99305.1.
PIR	A64462.
RefSeq	NP_248298.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58694.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJ1298.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB99305; AAB99305; MJ_1298.
GeneID	1452200.
KEGG	mja:MJ_1298.
Phylogenomic databases
eggNOG	COG0156.
KO	K00652.
OMA	EICCLAS.
ProtClustDB	CLSK876517.
Enzyme and pathway databases
UniPathway	UPA00078.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR001917. Aminotrans_II_pyridoxalP_BS. IPR004839. Aminotransferase_I/II. IPR004723. BioF. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00858. bioF. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BIOF_METJA

Accession

Primary (citable) accession number: Q58694

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program