Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q58692 (BIOB_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 358358Biotin synthase HAMAP-Rule MF_01694
PRO_0000185564

Sites

Metal binding651Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding721Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1421Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1741Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2331Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding3091Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q58692 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7EB75DC2ED9996BF

FASTA35840,722
        10         20         30         40         50         60 
MIFMEIETFL KKSLKNKIDF DDALYLYNNF SAIDLLYLAF KIKNRIKNNS KIKLCAIINA 

        70         80         90        100        110        120 
KSGKCKEDCI FCSQSIYSKC NIPIYPLKSK KEILEYAKKI IDECSKISSS IERGTLIGAE 

       130        140        150        160        170        180 
SPNLMDVGYP NRGFPLWVER FSIVTSGKKI NDDEFIEIVE AIELIKEETN LKVCCSLGLL 

       190        200        210        220        230        240 
DREKLKELKK LDVRIHNNLE ASKNYFKNIC STHSYEDKVK VIKEAKKLDL EVCSGGIFGL 

       250        260        270        280        290        300 
GESVEERIKM AFELKELGVD SVPINILHPI EGTKAYEKIK NGEIKPISVS DALKLIALYK 

       310        320        330        340        350 
IIMPYAEIRL AGGRIYNLRD FQSYALMVLD GLMVGNYLTT KGRCLEDDLK MIADFHSL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L77117 Genomic DNA. Translation: AAB99303.1.
PIRG64461.
RefSeqNP_248296.1. NC_000909.1.

3D structure databases

ProteinModelPortalQ58692.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243232.MJ1296.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB99303; AAB99303; MJ_1296.
GeneID1452198.
KEGGmja:MJ_1296.

Phylogenomic databases

eggNOGCOG0502.
KOK01012.
OMACKEDCIF.
PhylomeDBQ58692.

Enzyme and pathway databases

UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 2 hits.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_METJA
AccessionPrimary (citable) accession number: Q58692
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names