ID   TTDA_METJA              Reviewed;         285 AA.
AC   Q58690;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 110.
DE   RecName: Full=Putative L(+)-tartrate dehydratase subunit alpha {ECO:0000250|UniProtKB:P05847};
DE            Short=L-TTD alpha {ECO:0000250|UniProtKB:P05847};
DE            EC=4.2.1.32 {ECO:0000250|UniProtKB:P05847};
GN   OrderedLocusNames=MJ1294;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R,3R)-tartrate = H2O + oxaloacetate; Xref=Rhea:RHEA:15413,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:16452, ChEBI:CHEBI:30924; EC=4.2.1.32;
CC         Evidence={ECO:0000250|UniProtKB:P05847};
CC   -!- COFACTOR:
CC       Name=iron-sulfur cluster; Xref=ChEBI:CHEBI:30408;
CC         Evidence={ECO:0000250|UniProtKB:P05847};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000250|UniProtKB:P05847}.
CC   -!- SIMILARITY: Belongs to the class-I fumarase family. {ECO:0000305}.
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DR   EMBL; L77117; AAB99301.1; -; Genomic_DNA.
DR   PIR; E64461; E64461.
DR   RefSeq; WP_010870811.1; NC_000909.1.
DR   PDB; 7XKY; X-ray; 2.46 A; A=2-285.
DR   PDBsum; 7XKY; -.
DR   AlphaFoldDB; Q58690; -.
DR   SMR; Q58690; -.
DR   STRING; 243232.MJ_1294; -.
DR   PaxDb; 243232-MJ_1294; -.
DR   EnsemblBacteria; AAB99301; AAB99301; MJ_1294.
DR   GeneID; 1452196; -.
DR   KEGG; mja:MJ_1294; -.
DR   eggNOG; arCOG04407; Archaea.
DR   HOGENOM; CLU_041245_0_0_2; -.
DR   InParanoid; Q58690; -.
DR   OrthoDB; 371925at2157; -.
DR   PhylomeDB; Q58690; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0008730; F:L(+)-tartrate dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProt.
DR   InterPro; IPR004646; Fe-S_hydro-lyase_TtdA-typ_cat.
DR   NCBIfam; TIGR00722; ttdA_fumA_fumB; 1.
DR   PANTHER; PTHR30389; FUMARATE HYDRATASE-RELATED; 1.
DR   PANTHER; PTHR30389:SF17; L(+)-TARTRATE DEHYDRATASE SUBUNIT ALPHA-RELATED; 1.
DR   Pfam; PF05681; Fumerase; 1.
PE   1: Evidence at protein level;
KW   3D-structure; 4Fe-4S; Iron; Iron-sulfur; Lyase; Metal-binding;
KW   Reference proteome.
FT   CHAIN           1..285
FT                   /note="Putative L(+)-tartrate dehydratase subunit alpha"
FT                   /id="PRO_0000195661"
FT   BINDING         60
FT                   /ligand="iron-sulfur cluster"
FT                   /ligand_id="ChEBI:CHEBI:30408"
FT                   /evidence="ECO:0000250|UniProtKB:E9AE57"
FT   BINDING         182
FT                   /ligand="iron-sulfur cluster"
FT                   /ligand_id="ChEBI:CHEBI:30408"
FT                   /evidence="ECO:0000250|UniProtKB:E9AE57"
FT   BINDING         269
FT                   /ligand="iron-sulfur cluster"
FT                   /ligand_id="ChEBI:CHEBI:30408"
FT                   /evidence="ECO:0000250|UniProtKB:E9AE57"
SQ   SEQUENCE   285 AA;  31088 MW;  49BA64703BBC7ABC CRC64;
     MKISDVVVEL FREAAIYLPE DVKNALEEAY KKESSEISKN TLKAIIENNK IAEETQVPLC
     QDTGVPIVFL KIGKNINSSE IMKIIEEIKE GVKKATEEVP LRPNVVHPLT RENFKTNVGL
     NSPFINIEFD ESLDREIEII AFPKGAGSEN MSALKMLKPS DGIEGIKNFV LETIANAGGK
     PCPPIVVGIG IGGTADVALK LAKKALLRKI GERHRDKEIA NLEKELLEKI NSLGIGAMGL
     GGDITALDVF IEIAGCHTAS LPVGICIQCW ADRRAIKRIK LDAKL
//