Reviewed, UniProtKB/Swiss-Prot Q58690 (FUMA_METJA)

Names and origin

Protein names

Recommended name:
    Putative fumarate hydratase subunit alpha
      Short name=Fumarase
    EC=4.2.1.2

Gene names

Ordered Locus Names:

MJ1294

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O.
Cofactor	Iron-sulfur.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
Sequence similarities	Belongs to the class-I fumarase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW fumarate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Putative fumarate hydratase subunit alpha		PRO_0000195661
Sites
	Metal binding	60	1	Iron-sulfur By similarity
	Metal binding	182	1	Iron-sulfur By similarity
	Metal binding	269	1	Iron-sulfur By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58690-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 49BA64703BBC7ABC	FASTA	285	31,088
	10 20 30 40 50 60 MKISDVVVEL FREAAIYLPE DVKNALEEAY KKESSEISKN TLKAIIENNK IAEETQVPLC 70 80 90 100 110 120 QDTGVPIVFL KIGKNINSSE IMKIIEEIKE GVKKATEEVP LRPNVVHPLT RENFKTNVGL 130 140 150 160 170 180 NSPFINIEFD ESLDREIEII AFPKGAGSEN MSALKMLKPS DGIEGIKNFV LETIANAGGK 190 200 210 220 230 240 PCPPIVVGIG IGGTADVALK LAKKALLRKI GERHRDKEIA NLEKELLEKI NSLGIGAMGL 250 260 270 280 GGDITALDVF IEIAGCHTAS LPVGICIQCW ADRRAIKRIK LDAKL

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99301.1.
PIR	E64461.
RefSeq	NP_248294.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1452196.
GenomeReviews	Gene locus MJ1294 in contig L77117_GR.
KEGG	mja:MJ1294.
NMPDR	fig|243232.1.peg.1331.
TIGR	MJ1294.
Phylogenomic databases
HOGENOM	HBG548096.
OMA	GCHTASL.
Enzyme and pathway databases
BRENDA	4.2.1.2. 256362.
Family and domain databases
InterPro	IPR004646. Fe-S_hydro-lyase_TtdA-typ_cat. [Graphical view]
Pfam	PF05681. Fumerase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00722. ttdA_fumA_fumB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FUMA_METJA

Accession

Primary (citable) accession number: Q58690

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)