Q58660 (PURL_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	MJ1264

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	733 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 733	733	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100513
Regions
	Nucleotide binding	94 – 105	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q58660 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E0923221CB37722D	FASTA	733	80,866
	10 20 30 40 50 60 MDENDLKYIE KVLGRKPNHI ELAMFENLWS EHCAYRTSKK LLRMFAKTVN EKTSKNIVVG 70 80 90 100 110 120 IGDDAAVIRL KNDICLAIAM ESHNHPSYID PYNGAATGVG GIVRDVLSMG AKPIALLDPL 130 140 150 160 170 180 RFGDIFGKEG DKVRWLIEGV VKGIGDYGNR IGVPTVGGEC EFDSSFDYNN LVNVVCVGLV 190 200 210 220 230 240 KENEIITGKA KEPGLSLILI GSTGRDGIGG ASFASKDLTE ESEEERPSVQ VGDAFSEKCL 250 260 270 280 290 300 IDAVLEAVKT GKVKAMKDLG AAGLSGASSE MCYGGGVGCE LYLENVVLRE PLTPYEIMVS 310 320 330 340 350 360 ESQERMLLAV EPGSEEEIIE IFKKYELPAS VIGKTIPEKR IIAKYKGEVV VDLPLDLLCE 370 380 390 400 410 420 APLYDREGKE DLKEKEDDKE KIKMPEDLNA VLLKLLESPN ICSKEWIYQQ YDHEVQIRTV 430 440 450 460 470 480 VKPGKDAAVL RINEVYPMGI ALTTDCNSRY CKLNPYVGAV NAVAEAVRNL ATVGAEPIAM 490 500 510 520 530 540 LDNLNFGNPE RPERFWQLAE CIKGLADAAE FFEIPVVGGN VSLYNETVIE GKEHPINPTP 550 560 570 580 590 600 AIFVLGKVED VEKVPGVLDN KIKEGDILII TNETKDEMGG SEYYKVIHNT EEGRVPRVDL 610 620 630 640 650 660 EKEKKIYEEV REVVKEGLVS EAVDCSRGGL AVALAKMAVL NNIGLEVDLT EYNKNNLRDD 670 680 690 700 710 720 ILLFSETSGR IILAVRDENK DKVLSKLSSA YIIGKVGGSR LKIKINEKDV VNLDVEEMKK 730 RYYEAFPKMM GEL

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99270.1.
PIR	G64457.
RefSeq	NP_248260.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58660.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1452162.
GenomeReviews	Gene locus MJ1264 in contig L77117_GR.
KEGG	mja:MJ_1264.
NMPDR	fig|243232.1.peg.1297.
TIGR	MJ1264.
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1264-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_METJA

Accession

Primary (citable) accession number: Q58660

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program