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Q58634

- PSB_METJA

UniProt

Q58634 - PSB_METJA

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Protein

Proteasome subunit beta

Gene
psmB, MJ1237
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The M.jannaschii proteasome is able to cleave oligopeptides after Glu, Asp, Tyr, Phe, Trp, slightly after Arg, but not after Ala. Thus, displays caspase-like and chymotrypsin-like activities and low level of trypsin-like activity.1 Publication

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.1 Publication

Enzyme regulationi

The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei7 – 71Nucleophile By similarity

GO - Molecular functioni

  1. endopeptidase activity Source: UniProtKB
  2. threonine-type endopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. proteasomal protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Protein family/group databases

MEROPSiT01.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta (EC:3.4.25.1)
Alternative name(s):
20S proteasome beta subunit
Proteasome core protein PsmB
Gene namesi
Name:psmB
Ordered Locus Names:MJ1237
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. proteasome core complex, beta-subunit complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 66Removed in mature form; by autocatalysis By similarityPRO_0000026661
Chaini7 – 224218Proteasome subunit betaUniRule annotationPRO_0000026662Add
BLAST

Keywords - PTMi

Autocatalytic cleavage, Zymogen

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.2 Publications

Protein-protein interaction databases

STRINGi243232.MJ1237.

Structurei

Secondary structure

1
224
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 143
Beta strandi17 – 215
Beta strandi32 – 343
Beta strandi40 – 445
Beta strandi47 – 504
Helixi58 – 7518
Helixi82 – 9514
Turni96 – 1005
Turni141 – 1433
Helixi162 – 17211
Beta strandi184 – 1874
Helixi197 – 20610

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H4PX-ray4.10a/b/c/d/e/f/g/h/i/j/k/l/m/n7-224[»]
ProteinModelPortaliQ58634.
SMRiQ58634. Positions 7-208.

Miscellaneous databases

EvolutionaryTraceiQ58634.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.

Phylogenomic databases

eggNOGiCOG0638.
KOiK03433.
OMAiGYYIAHR.
PhylomeDBiQ58634.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_02113_A. Proteasome_B_A.
InterProiIPR029055. Ntn_hydrolases_N.
IPR019983. Pept_T1A_Psome_bsu_arc.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03634. arc_protsome_B. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58634-1 [UniParc]FASTAAdd to Basket

« Hide

MDVMKGTTTV GLICDDAVIL ATDKRASLGN LVADKEAKKL YKIDDYIAMT    50
IAGSVGDAQA IVRLLIAEAK LYKMRTGRNI PPLACATLLS NILHSSRMFP 100
FLTQIIIGGY DLLEGAKLFS LDPLGGMNEE KTFTATGSGS PIAYGVLEAG 150
YDRDMSVEEG IKLALNALKS AMERDTFSGN GISLAVITKD GVKIFEDEEI 200
EKILDSMKAK PKKKTTKRSR RKSK 224
Length:224
Mass (Da):24,285
Last modified:November 1, 1997 - v1
Checksum:i45B2A0D1EAEF7A58
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99241.1.
PIRiD64454.
RefSeqiNP_248232.1. NC_000909.1.
WP_010870749.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99241; AAB99241; MJ_1237.
GeneIDi1452133.
KEGGimja:MJ_1237.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99241.1 .
PIRi D64454.
RefSeqi NP_248232.1. NC_000909.1.
WP_010870749.1. NC_000909.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3H4P X-ray 4.10 a/b/c/d/e/f/g/h/i/j/k/l/m/n 7-224 [» ]
ProteinModelPortali Q58634.
SMRi Q58634. Positions 7-208.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJ1237.

Protein family/group databases

MEROPSi T01.002.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB99241 ; AAB99241 ; MJ_1237 .
GeneIDi 1452133.
KEGGi mja:MJ_1237.

Phylogenomic databases

eggNOGi COG0638.
KOi K03433.
OMAi GYYIAHR.
PhylomeDBi Q58634.

Miscellaneous databases

EvolutionaryTracei Q58634.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
HAMAPi MF_02113_A. Proteasome_B_A.
InterProi IPR029055. Ntn_hydrolases_N.
IPR019983. Pept_T1A_Psome_bsu_arc.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
[Graphical view ]
PRINTSi PR00141. PROTEASOME.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR03634. arc_protsome_B. 1 hit.
PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  2. "Biochemical and physical properties of the Methanococcus jannaschii 20S proteasome and PAN, a homolog of the ATPase (Rpt) subunits of the eucaryal 26S proteasome."
    Wilson H.L., Ou M.S., Aldrich H.C., Maupin-Furlow J.
    J. Bacteriol. 182:1680-1692(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, INTERACTION WITH PAN.
  3. "Structural insights into the regulatory particle of the proteasome from Methanocaldococcus jannaschii."
    Zhang F., Hu M., Tian G., Zhang P., Finley D., Jeffrey P.D., Shi Y.
    Mol. Cell 34:473-484(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.1 ANGSTROMS) IN COMPLEX WITH ALPHA SUBUNIT, GATED STRUCTURE, SUBUNIT.

Entry informationi

Entry nameiPSB_METJA
AccessioniPrimary (citable) accession number: Q58634
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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