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Q58634

- PSB_METJA

UniProt

Q58634 - PSB_METJA

Protein

Proteasome subunit beta

Gene

psmB

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The M.jannaschii proteasome is able to cleave oligopeptides after Glu, Asp, Tyr, Phe, Trp, slightly after Arg, but not after Ala. Thus, displays caspase-like and chymotrypsin-like activities and low level of trypsin-like activity.1 PublicationUniRule annotation

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.1 PublicationUniRule annotation

    Enzyme regulationi

    The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei7 – 71NucleophileUniRule annotation

    GO - Molecular functioni

    1. endopeptidase activity Source: UniProtKB
    2. threonine-type endopeptidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. proteasomal protein catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Protein family/group databases

    MEROPSiT01.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit betaUniRule annotation (EC:3.4.25.1UniRule annotation)
    Alternative name(s):
    20S proteasome beta subunitUniRule annotation
    Proteasome core protein PsmBUniRule annotation
    Gene namesi
    Name:psmBUniRule annotation
    Ordered Locus Names:MJ1237
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. proteasome core complex, beta-subunit complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 66Removed in mature form; by autocatalysisUniRule annotationPRO_0000026661
    Chaini7 – 224218Proteasome subunit betaPRO_0000026662Add
    BLAST

    Keywords - PTMi

    Autocatalytic cleavage, Zymogen

    Interactioni

    Subunit structurei

    The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.2 PublicationsUniRule annotation

    Protein-protein interaction databases

    STRINGi243232.MJ1237.

    Structurei

    Secondary structure

    1
    224
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi12 – 143
    Beta strandi17 – 215
    Beta strandi32 – 343
    Beta strandi40 – 445
    Beta strandi47 – 504
    Helixi58 – 7518
    Helixi82 – 9514
    Turni96 – 1005
    Turni141 – 1433
    Helixi162 – 17211
    Beta strandi184 – 1874
    Helixi197 – 20610

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3H4PX-ray4.10a/b/c/d/e/f/g/h/i/j/k/l/m/n7-224[»]
    ProteinModelPortaliQ58634.
    SMRiQ58634. Positions 7-208.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ58634.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    KOiK03433.
    OMAiGYYIAHR.
    PhylomeDBiQ58634.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    HAMAPiMF_02113_A. Proteasome_B_A.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR019983. Pept_T1A_Psome_bsu_arc.
    IPR000243. Pept_T1A_subB.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    PRINTSiPR00141. PROTEASOME.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR03634. arc_protsome_B. 1 hit.
    PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q58634-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDVMKGTTTV GLICDDAVIL ATDKRASLGN LVADKEAKKL YKIDDYIAMT    50
    IAGSVGDAQA IVRLLIAEAK LYKMRTGRNI PPLACATLLS NILHSSRMFP 100
    FLTQIIIGGY DLLEGAKLFS LDPLGGMNEE KTFTATGSGS PIAYGVLEAG 150
    YDRDMSVEEG IKLALNALKS AMERDTFSGN GISLAVITKD GVKIFEDEEI 200
    EKILDSMKAK PKKKTTKRSR RKSK 224
    Length:224
    Mass (Da):24,285
    Last modified:November 1, 1997 - v1
    Checksum:i45B2A0D1EAEF7A58
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99241.1.
    PIRiD64454.
    RefSeqiNP_248232.1. NC_000909.1.
    WP_010870749.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB99241; AAB99241; MJ_1237.
    GeneIDi1452133.
    KEGGimja:MJ_1237.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99241.1 .
    PIRi D64454.
    RefSeqi NP_248232.1. NC_000909.1.
    WP_010870749.1. NC_000909.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3H4P X-ray 4.10 a/b/c/d/e/f/g/h/i/j/k/l/m/n 7-224 [» ]
    ProteinModelPortali Q58634.
    SMRi Q58634. Positions 7-208.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ1237.

    Protein family/group databases

    MEROPSi T01.002.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB99241 ; AAB99241 ; MJ_1237 .
    GeneIDi 1452133.
    KEGGi mja:MJ_1237.

    Phylogenomic databases

    eggNOGi COG0638.
    KOi K03433.
    OMAi GYYIAHR.
    PhylomeDBi Q58634.

    Miscellaneous databases

    EvolutionaryTracei Q58634.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    HAMAPi MF_02113_A. Proteasome_B_A.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR019983. Pept_T1A_Psome_bsu_arc.
    IPR000243. Pept_T1A_subB.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    PRINTSi PR00141. PROTEASOME.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR03634. arc_protsome_B. 1 hit.
    PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
    2. "Biochemical and physical properties of the Methanococcus jannaschii 20S proteasome and PAN, a homolog of the ATPase (Rpt) subunits of the eucaryal 26S proteasome."
      Wilson H.L., Ou M.S., Aldrich H.C., Maupin-Furlow J.
      J. Bacteriol. 182:1680-1692(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, INTERACTION WITH PAN.
    3. "Structural insights into the regulatory particle of the proteasome from Methanocaldococcus jannaschii."
      Zhang F., Hu M., Tian G., Zhang P., Finley D., Jeffrey P.D., Shi Y.
      Mol. Cell 34:473-484(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.1 ANGSTROMS) IN COMPLEX WITH ALPHA SUBUNIT, GATED STRUCTURE, SUBUNIT.

    Entry informationi

    Entry nameiPSB_METJA
    AccessioniPrimary (citable) accession number: Q58634
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3