Translation initiation factor 5A - Methanocaldococcus jannaschii

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Reviewed, UniProtKB/Swiss-Prot Q58625 (IF5A_METJA)

Last modified November 3, 2009. Version 72. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Translation initiation factor 5A
Alternative name(s):
    eIF-5A
    Hypusine-containing protein

Gene names

Name:	eif5a
Ordered Locus Names:	MJ1228

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

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Protein attributes

Sequence length	132 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Functions by promoting the formation of the first peptide bond. HAMAP MF_00085
Subcellular location	Cytoplasm. HAMAP MF_00085
Sequence similarities	Belongs to the eIF-5A family.

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Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Initiation factor
PTM	Hypusine
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-lysine modification to hypusine Inferred from electronic annotation. Source: InterPro positive regulation of translational elongation Inferred from electronic annotation. Source: InterPro positive regulation of translational termination Inferred from electronic annotation. Source: InterPro translational frameshifting Inferred from electronic annotation. Source: InterPro translational initiation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro ribosome binding Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: InterPro translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 132

132

Translation initiation factor 5A HAMAP MF_00085

PRO_0000142493

Amino acid modifications

Modified residue

Hypusine HAMAP MF_00085

Secondary structure

132

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q58625-1 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: 5B0F48905B0DCF69
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FASTA

132

14,180

        10         20         30         40         50         60 
MPGTKQVNVG SLKVGQYVMI DGVPCEIVDI SVSKPGKHGG AKARVVGIGI FEKVKKEFVA 

        70         80         90        100        110        120 
PTSSKVEVPI IDRRKGQVLA IMGDMVQIMD LQTYETLELP IPEGIEGLEP GGEVEYIEAV 

       130 
GQYKITRVIG GK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Universally conserved translation initiation factors." Kyrpides N.C., Woese C.R. Proc. Natl. Acad. Sci. U.S.A. 95:224-228(1998) [PubMed: 9419357] [Abstract] Cited for: SIMILARITY.
[3]	"Crystal structures of eukaryotic translation initiation factor 5A from Methanococcus jannaschii at 1.8-A resolution." Kim K.K., Hung L.W., Yokota H., Kim R., Kim S.H. Proc. Natl. Acad. Sci. U.S.A. 95:10419-10424(1998) [PubMed: 9724718] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), HYPUSINE AT LYS-37.

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Cross-references

Sequence databases

L77117 Genomic DNA. Translation: AAB99231.1.

RefSeq

NP_248223.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EIF	X-ray	1.90	A	1-132	[»]
2EIF	X-ray	1.80	A	1-132	[»]

ModBase

Search...

Genome annotation databases

GeneID

1452124.

GenomeReviews

Gene locus MJ1228 in contig L77117_GR.

KEGG

mja:MJ1228.

NMPDR

fig|243232.1.peg.1260.

TIGR

MJ1228.

Phylogenomic databases

HOGENOM

Q58625.

OMA

VIDGEPC.

Enzyme and pathway databases

BioCyc

MJAN243232:MJ_1228-MON.

Family and domain databases

HAMAP

MF_00085.
[Tree]

InterPro

IPR005824. KOW.
IPR019769. Trans_elong_IF5A_hypusine_site.
IPR001884. Transl_elong_IF5A.
IPR020189. Transl_elong_IF5A_C.
IPR014722. Transl_SH3-like_sub.
[Graphical view]

Gene3D

G3DSA:2.30.30.30. Ribosomal_L2. 1 hit.

PANTHER

PTHR11673. EIF5A_hypusine. 1 hit.

Pfam

PF01287. eIF-5a. 1 hit.
PF00467. KOW. 1 hit.
[Graphical view]

PIRSF

PIRSF003025. eIF5A. 1 hit.

TIGRFAMs

TIGR00037. eIF_5A. 1 hit.

PROSITE

PS00302. IF5A_HYPUSINE. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

IF5A_METJA

Accession

Primary (citable) accession number: Q58625

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	November 3, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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