Q58606 (MTM6_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase MjaVI
Short name=M.MjaVI
EC=2.1.1.113

Alternative name(s):
N-4 cytosine-specific methyltransferase MjaVI

Gene names

Name:	mjaVIM
Ordered Locus Names:	MJ1209

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the MjaVI endonuclease Potential.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Modification methylase MjaVI		PRO_0000087939

Sequences

	Sequence		Length	Mass (Da)
	Q58606 [UniParc]. Last modified June 1, 1998. Version 2. Checksum: 6DB2BAEEAEAEBC10	FASTA	194	22,461
	10 20 30 40 50 60 MKVIIVKIWN KINGITLIND DFLNVDLPNE SIDLIVTSPP YNVGIDYNQH DDTIPYEEYL 70 80 90 100 110 120 DWTKQWLKKA LTLLKKDGRL CLNIPLDKNK GGIKPVYADI VKIALDVGFK YQTTIIWNEQ 130 140 150 160 170 180 NISRRTAWGS FMSASAPYVI APVETIVVLY KESWKKLSKG ESDITKEEFI EWTNGLWTFP 190 GESKKELDIQ HHFR

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99220.1.
RefSeq	YP_003727984.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58606.
ModBase	Search...
Protein family/group databases
REBASE	3893. M.MjaVI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9348047.
GenomeReviews	Gene locus MJ1209 in contig L77117_GR.
KEGG	mja:MJ_1209.
TIGR	MJ1209.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1209-MONOMER.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR017985. MeTrfase_CN4_CS. IPR001091. RM_Methylase. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTM6_METJA

Accession

Primary (citable) accession number: Q58606

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 1, 1998
Last modified:	October 19, 2011
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program