Q58592 (VHUA_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F420-non-reducing hydrogenase vhu subunit A
EC=1.12.99.-

Gene names

Name:	vhuA
Ordered Locus Names:	MJ1192

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor	Nickel Potential.
Subunit structure	The F420-non-reducing hydrogenase vhu is composed of four subunits; vhuA, vhuD, vhuG and vhuU By similarity.
Miscellaneous	The large subunit of vhu is split into vhuA and vhuU. Each contributes two ligands to the [NiFeSe] center.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	F420-non-reducing hydrogenase vhu subunit A		PRO_0000199730
Sites
	Metal binding	61	1	Nickel Potential
	Metal binding	64	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	Q58592 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0BF7333C53360FA4	FASTA	418	46,652
	10 20 30 40 50 60 MGKIVIEPLS RLEGHGKVTI TLDENGKPKD VKLHITALRG FEQFVVGRPA EEVPRIVPRI 70 80 90 100 110 120 CGICQTAHHL ASVKAIDAAW GVEIPEPAKK LRELMHIGNM IHSHALHFYF LAAPDFVLGP 130 140 150 160 170 180 DADPAIRNIV GVIDKAPDVA KQAIALRKFG QKIVEAVGGK AIHPVTGIPG GQAKRLTEEE 190 200 210 220 230 240 RDELLKDADQ MIEYAKNGVE LIKQLNEQYM EQIKTLGVID TYYLGLVKDG KHNFYDDTLR 250 260 270 280 290 300 FLSPDGKEKV EFKPEEYLNY IGEYVVPYNY VKHPYYKKVG YPEGVYRVGP LAMLNVCDEM 310 320 330 340 350 360 ETPLAEEYRK EFLEIFGFPA NQSLAYNHAR LIELVEACEK AKILLEDNDI TSDDIKADVE 370 380 390 400 410 PKAGNGVGVV YAPRGVLIHN YETDENGIVV KANMIVATTH NVPTMEKAIQ QAAQVIFK

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99194.1.
PIR	G64448.
RefSeq	NP_248187.2. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58592.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1452611.
GenomeReviews	Gene locus MJ1192 in contig L77117_GR.
TIGR	MJ1192.
Phylogenomic databases
HOGENOM	HBG287963.
OMA	MEKAIQQ.
ProtClustDB	CLSK2754647.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1192-MONOMER.
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
Pfam	PF00374. NiFeSe_Hases. 2 hits. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VHUA_METJA

Accession

Primary (citable) accession number: Q58592

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	November 1, 1996
Last modified:	October 19, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program