Reviewed, UniProtKB/Swiss-Prot Q58549 (ADPP_METJA)

Names and origin

Protein names

Recommended name:
    ADP-ribose pyrophosphatase
    EC=3.6.1.13
Alternative name(s):
    ADP-ribose diphosphatase
    Adenosine diphosphoribose pyrophosphatase
      Short name=ADPR-PPase
    ADP-ribose phosphohydrolase

Gene names

Name:	nudF
Ordered Locus Names:	MJ1149

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ADP-ribose + H2O = AMP + D-ribose 5-phosphate.
Cofactor	Magnesium.
Sequence similarities	Belongs to the Nudix hydrolase family. NudF subfamily.
Biophysicochemical properties	pH dependence: Optimum pH is 4.85. Temperature dependence: Thermostable.

Ontologies

Keywords
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	ADP-ribose diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	ADP-ribose pyrophosphatase		PRO_0000057047
Regions
	Motif	74 – 95	22	Nudix box

Sequences

	Sequence		Length	Mass (Da)
	Q58549-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1966F84445658B00	FASTA	169	19,144
	10 20 30 40 50 60 MAKKCFCISG KIFALNLFGK RYSKKIIKKR DLKKYRLYLH PAVAVDGIIE KDNKILLIKR 70 80 90 100 110 120 KNNPFKGCFA LPGGFVECGE TVEEAVVREI KEETGLIPKV KSLLGVYSSP DRDPRGHVIS 130 140 150 160 IVFILDVIGG ELKAGDDAKE AEFFDLNNLP KLAFDHEKII KDYMRWKNG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Identification and characterization of the Nudix hydrolase from the Archaeon, Methanococcus jannaschii, as a highly specific ADP-ribose pyrophosphatase." Sheikh S., O'Handley S.F., Dunn C.A., Bessman M.J. J. Biol. Chem. 273:20924-20928(1998) [PubMed: 9694840] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB99149.1.
PIR	D64443.
RefSeq	NP_248141.1.
3D structure databases
HSSP	HSSP built from PDB template 1TUM based on UniProtKB P08337.
ModBase	Search...
Genome annotation databases
GeneID	1452045.
GenomeReviews	Gene locus MJ1149 in contig L77117_GR.
KEGG	mja:MJ1149.
NMPDR	fig|243232.1.peg.1178.
TIGR	MJ1149.
Phylogenomic databases
HOGENOM	Q58549.
OMA	Q58549. HTVTVAF.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1149-MON.
BRENDA	3.6.1.13. 256362.
Family and domain databases
InterPro	IPR000086. NUDIX_hydrolase_core. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
PROSITE	PS00893. NUDIX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADPP_METJA

Accession

Primary (citable) accession number: Q58549

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	May 5, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)