Q58549 (ADPP_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-ribose pyrophosphatase
EC=3.6.1.13

Alternative name(s):
ADP-ribose diphosphatase
ADP-ribose phosphohydrolase
Adenosine diphosphoribose pyrophosphatase
Short name=ADPR-PPase

Gene names

Name:	nudF
Ordered Locus Names:	MJ1149

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ADP-ribose + H2O = AMP + D-ribose 5-phosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Sequence similarities	Belongs to the Nudix hydrolase family. NudF subfamily. Contains 1 nudix hydrolase domain.
Biophysicochemical properties	pH dependence: Optimum pH is 4.85. Temperature dependence: Thermostable.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ADP-ribose diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	ADP-ribose pyrophosphatase		PRO_0000057047
Regions
	Region	12 – 13	2	Substrate binding By similarity
	Region	35 – 36	2	Substrate binding; shared with dimeric partner By similarity
	Region	109 – 111	3	Substrate binding; shared with dimeric partner By similarity
	Motif	74 – 95	22	Nudix box
Sites
	Active site	140	1	Proton acceptor Potential
	Metal binding	73	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	89	1	Magnesium 2 By similarity
	Metal binding	89	1	Magnesium 3 By similarity
	Metal binding	93	1	Magnesium 1 By similarity
	Metal binding	93	1	Magnesium 3 By similarity
	Metal binding	142	1	Magnesium 3 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58549 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1966F84445658B00	FASTA	169	19,144
	10 20 30 40 50 60 MAKKCFCISG KIFALNLFGK RYSKKIIKKR DLKKYRLYLH PAVAVDGIIE KDNKILLIKR 70 80 90 100 110 120 KNNPFKGCFA LPGGFVECGE TVEEAVVREI KEETGLIPKV KSLLGVYSSP DRDPRGHVIS 130 140 150 160 IVFILDVIGG ELKAGDDAKE AEFFDLNNLP KLAFDHEKII KDYMRWKNG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Identification and characterization of the Nudix hydrolase from the Archaeon, Methanococcus jannaschii, as a highly specific ADP-ribose pyrophosphatase." Sheikh S., O'Handley S.F., Dunn C.A., Bessman M.J. J. Biol. Chem. 273:20924-20928(1998) [PubMed: 9694840] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99149.1.
PIR	D64443.
RefSeq	NP_248141.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58549.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1452045.
GenomeReviews	Gene locus MJ1149 in contig L77117_GR.
KEGG	mja:MJ_1149.
NMPDR	fig|243232.1.peg.1178.
TIGR	MJ1149.
Phylogenomic databases
HOGENOM	HBG741910.
OMA	EYGETVE.
ProtClustDB	CLSK2517692.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1149-MONOMER.
Family and domain databases
InterPro	IPR020476. Nudix_hydrolase. IPR020084. NUDIX_hydrolase_CS. IPR000086. NUDIX_hydrolase_dom. IPR015797. NUDIX_hydrolase_dom-like. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
KO	K03574.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
PRINTS	PR00502. NUDIXFAMILY.
SUPFAM	SSF55811. NUDIX_hydrolase. 1 hit.
PROSITE	PS51462. NUDIX. 1 hit. PS00893. NUDIX_BOX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADPP_METJA

Accession

Primary (citable) accession number: Q58549

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program