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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (apgM2), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (apgM1)
  4. Enolase (eno)
  5. Pyruvate kinase (MJ0108)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei114NAD; via amide nitrogenBy similarity1
Active sitei144NucleophileBy similarity1
Binding sitei172NADBy similarity1
Binding sitei307NAD; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 13NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BRENDAi1.2.1.59. 3260.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.59)
Short name:
GAPDH
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase
Gene namesi
Name:gap
Ordered Locus Names:MJ1146
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001457231 – 343Glyceraldehyde-3-phosphate dehydrogenaseAdd BLAST343

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi243232.MJ_1146.

Structurei

Secondary structure

1343
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi12 – 23Combined sources12
Beta strandi24 – 36Combined sources13
Helixi39 – 46Combined sources8
Beta strandi51 – 55Combined sources5
Helixi58 – 66Combined sources9
Helixi75 – 77Combined sources3
Helixi79 – 81Combined sources3
Beta strandi83 – 87Combined sources5
Helixi93 – 100Combined sources8
Turni101 – 106Combined sources6
Beta strandi108 – 111Combined sources4
Helixi117 – 119Combined sources3
Beta strandi120 – 124Combined sources5
Turni126 – 128Combined sources3
Helixi130 – 133Combined sources4
Beta strandi137 – 141Combined sources5
Helixi144 – 157Combined sources14
Beta strandi160 – 174Combined sources15
Beta strandi188 – 195Combined sources8
Helixi198 – 205Combined sources8
Helixi207 – 209Combined sources3
Beta strandi212 – 221Combined sources10
Beta strandi226 – 236Combined sources11
Helixi240 – 249Combined sources10
Beta strandi253 – 256Combined sources4
Helixi258 – 260Combined sources3
Helixi265 – 275Combined sources11
Helixi278 – 280Combined sources3
Beta strandi284 – 288Combined sources5
Helixi289 – 291Combined sources3
Beta strandi293 – 295Combined sources3
Beta strandi298 – 305Combined sources8
Turni307 – 311Combined sources5
Helixi312 – 322Combined sources11
Helixi329 – 340Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YYYX-ray1.85A/B1-343[»]
ProteinModelPortaliQ58546.
SMRiQ58546.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ58546.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni143 – 145Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni198 – 199Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00493. Archaea.
COG0057. LUCA.
InParanoidiQ58546.
KOiK00150.
OMAiVTVPSHH.
PhylomeDBiQ58546.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00559. G3P_dehdrog_arch. 1 hit.
InterProiIPR000846. DapB_N.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01113. DapB_N. 1 hit.
PF02800. Gp_dh_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q58546-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAKVLINGY GSIGKRVADA VSMQDDMEVI GVTKTKPDFE ARLAVEKGYK
60 70 80 90 100
LFVAIPDNER VKLFEDAGIP VEGTILDIIE DADIVVDGAP KKIGKQNLEN
110 120 130 140 150
IYKPHKVKAI LQGGEKAKDV EDNFNALWSY NRCYGKDYVR VVSCNTTGLC
160 170 180 190 200
RILYAINSIA DIKKARIVLV RRAADPNDDK TGPVNAITPN PVTVPSHHGP
210 220 230 240 250
DVVSVVPEFE GKILTSAVIV PTTLMHMHTL MVEVDGDVSR DDILEAIKKT
260 270 280 290 300
PRIITVRAED GFSSTAKIIE YGRDLGRLRY DINELVVWEE SINVLENEIF
310 320 330 340
LMQAVHQESI VIPENIDCIR AMLQMEEDNF KSIEKTNKAM GIQ
Length:343
Mass (Da):38,102
Last modified:November 1, 1996 - v1
Checksum:i81EB5810A9C838C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99147.1.
PIRiA64443.
RefSeqiWP_010870657.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99147; AAB99147; MJ_1146.
GeneIDi1452042.
KEGGimja:MJ_1146.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99147.1.
PIRiA64443.
RefSeqiWP_010870657.1. NC_000909.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YYYX-ray1.85A/B1-343[»]
ProteinModelPortaliQ58546.
SMRiQ58546.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_1146.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99147; AAB99147; MJ_1146.
GeneIDi1452042.
KEGGimja:MJ_1146.

Phylogenomic databases

eggNOGiarCOG00493. Archaea.
COG0057. LUCA.
InParanoidiQ58546.
KOiK00150.
OMAiVTVPSHH.
PhylomeDBiQ58546.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
BRENDAi1.2.1.59. 3260.

Miscellaneous databases

EvolutionaryTraceiQ58546.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00559. G3P_dehdrog_arch. 1 hit.
InterProiIPR000846. DapB_N.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01113. DapB_N. 1 hit.
PF02800. Gp_dh_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3P_METJA
AccessioniPrimary (citable) accession number: Q58546
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.