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Q58546 (G3P_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glyceraldehyde-3-phosphate dehydrogenase
Short name=GAPDH
EC=1.2.1.59
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase
Gene names
Name:gap
Ordered Locus Names:MJ1146
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length343 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559

Subunit structure

Homotetramer By similarity. HAMAP MF_00559

Subcellular location

Cytoplasm By similarity HAMAP MF_00559.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
NADP
   Molecular functionOxidoreductase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionNAD binding

Inferred from electronic annotation. Source: InterPro

NADP binding

Inferred from electronic annotation. Source: InterPro

glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 343343Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559
PRO_0000145723

Regions

Nucleotide binding12 – 132NAD By similarity
Region143 – 1453Glyceraldehyde 3-phosphate binding By similarity
Region198 – 1992Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site1441Nucleophile By similarity
Binding site1141NAD; via amide nitrogen By similarity
Binding site1721NAD By similarity
Binding site3071NAD; via carbonyl oxygen By similarity

Secondary structure

1 .................................................................... 343
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q58546 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 81EB5810A9C838C5

FASTA34338,102
        10         20         30         40         50         60 
MPAKVLINGY GSIGKRVADA VSMQDDMEVI GVTKTKPDFE ARLAVEKGYK LFVAIPDNER 

        70         80         90        100        110        120 
VKLFEDAGIP VEGTILDIIE DADIVVDGAP KKIGKQNLEN IYKPHKVKAI LQGGEKAKDV 

       130        140        150        160        170        180 
EDNFNALWSY NRCYGKDYVR VVSCNTTGLC RILYAINSIA DIKKARIVLV RRAADPNDDK 

       190        200        210        220        230        240 
TGPVNAITPN PVTVPSHHGP DVVSVVPEFE GKILTSAVIV PTTLMHMHTL MVEVDGDVSR 

       250        260        270        280        290        300 
DDILEAIKKT PRIITVRAED GFSSTAKIIE YGRDLGRLRY DINELVVWEE SINVLENEIF 

       310        320        330        340 
LMQAVHQESI VIPENIDCIR AMLQMEEDNF KSIEKTNKAM GIQ

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L77117 Genomic DNA. Translation: AAB99147.1.
PIRA64443.
RefSeqNP_248138.1. NC_000909.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2YYYX-ray1.85A/B1-343[»]
ProteinModelPortalQ58546.
SMRQ58546. Positions 2-343.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1452042.
GenomeReviewsGene locus MJ1146 in contig L77117_GR.
KEGGmja:MJ_1146.
NMPDRfig|243232.1.peg.1175.
TIGRMJ1146.

Phylogenomic databases

HOGENOMHBG392099.
OMAVPSHHGP.
ProtClustDBPRK04207.

Enzyme and pathway databases

BioCycMJAN243232:MJ_1146-MONOMER.

Family and domain databases

HAMAPMF_00559. G3P_dehdrog_arch.
[Tree]
InterProIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00150.
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000149. GAP_DH. 1 hit.
SMARTSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG3P_METJA
AccessionPrimary (citable) accession number: Q58546
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 16, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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