Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei114 – 1141NAD; via amide nitrogenBy similarity
Active sitei144 – 1441NucleophileBy similarity
Binding sitei172 – 1721NADBy similarity
Binding sitei307 – 3071NAD; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 132NADBy similarity

GO - Molecular functioni

  1. glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Source: UniProtKB-EC
  2. glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Source: UniProtKB-HAMAP
  3. NAD binding Source: InterPro
  4. NADP binding Source: InterPro

GO - Biological processi

  1. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BRENDAi1.2.1.59. 3260.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.59)
Short name:
GAPDH
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase
Gene namesi
Name:gap
Ordered Locus Names:MJ1146
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343Glyceraldehyde-3-phosphate dehydrogenasePRO_0000145723Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi243232.MJ1146.

Structurei

Secondary structure

1
343
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Helixi12 – 2312Combined sources
Beta strandi24 – 3613Combined sources
Helixi39 – 468Combined sources
Beta strandi51 – 555Combined sources
Helixi58 – 669Combined sources
Helixi75 – 773Combined sources
Helixi79 – 813Combined sources
Beta strandi83 – 875Combined sources
Helixi93 – 1008Combined sources
Turni101 – 1066Combined sources
Beta strandi108 – 1114Combined sources
Helixi117 – 1193Combined sources
Beta strandi120 – 1245Combined sources
Turni126 – 1283Combined sources
Helixi130 – 1334Combined sources
Beta strandi137 – 1415Combined sources
Helixi144 – 15714Combined sources
Beta strandi160 – 17415Combined sources
Beta strandi188 – 1958Combined sources
Helixi198 – 2058Combined sources
Helixi207 – 2093Combined sources
Beta strandi212 – 22110Combined sources
Beta strandi226 – 23611Combined sources
Helixi240 – 24910Combined sources
Beta strandi253 – 2564Combined sources
Helixi258 – 2603Combined sources
Helixi265 – 27511Combined sources
Helixi278 – 2803Combined sources
Beta strandi284 – 2885Combined sources
Helixi289 – 2913Combined sources
Beta strandi293 – 2953Combined sources
Beta strandi298 – 3058Combined sources
Turni307 – 3115Combined sources
Helixi312 – 32211Combined sources
Helixi329 – 34012Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YYYX-ray1.85A/B1-343[»]
ProteinModelPortaliQ58546.
SMRiQ58546. Positions 2-343.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ58546.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni143 – 1453Glyceraldehyde 3-phosphate bindingBy similarity
Regioni198 – 1992Glyceraldehyde 3-phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0057.
InParanoidiQ58546.
KOiK00150.
OMAiVTVPSHH.
PhylomeDBiQ58546.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00559. G3P_dehdrog_arch.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q58546-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAKVLINGY GSIGKRVADA VSMQDDMEVI GVTKTKPDFE ARLAVEKGYK
60 70 80 90 100
LFVAIPDNER VKLFEDAGIP VEGTILDIIE DADIVVDGAP KKIGKQNLEN
110 120 130 140 150
IYKPHKVKAI LQGGEKAKDV EDNFNALWSY NRCYGKDYVR VVSCNTTGLC
160 170 180 190 200
RILYAINSIA DIKKARIVLV RRAADPNDDK TGPVNAITPN PVTVPSHHGP
210 220 230 240 250
DVVSVVPEFE GKILTSAVIV PTTLMHMHTL MVEVDGDVSR DDILEAIKKT
260 270 280 290 300
PRIITVRAED GFSSTAKIIE YGRDLGRLRY DINELVVWEE SINVLENEIF
310 320 330 340
LMQAVHQESI VIPENIDCIR AMLQMEEDNF KSIEKTNKAM GIQ
Length:343
Mass (Da):38,102
Last modified:November 1, 1996 - v1
Checksum:i81EB5810A9C838C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99147.1.
PIRiA64443.
RefSeqiNP_248138.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99147; AAB99147; MJ_1146.
GeneIDi1452042.
KEGGimja:MJ_1146.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99147.1.
PIRiA64443.
RefSeqiNP_248138.1. NC_000909.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YYYX-ray1.85A/B1-343[»]
ProteinModelPortaliQ58546.
SMRiQ58546. Positions 2-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ1146.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99147; AAB99147; MJ_1146.
GeneIDi1452042.
KEGGimja:MJ_1146.

Phylogenomic databases

eggNOGiCOG0057.
InParanoidiQ58546.
KOiK00150.
OMAiVTVPSHH.
PhylomeDBiQ58546.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
BRENDAi1.2.1.59. 3260.

Miscellaneous databases

EvolutionaryTraceiQ58546.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00559. G3P_dehdrog_arch.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiG3P_METJA
AccessioniPrimary (citable) accession number: Q58546
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.