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Reviewed, UniProtKB/Swiss-Prot Q58540 (COMB_METJA)

Last modified June 16, 2009. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    2-phosphosulfolactate phosphatase
    EC=3.1.3.71
Gene names
Name: comB
Ordered Locus Names: MJ1140
OrganismMethanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]
Taxonomic identifier2190 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Hydrolyzes both enantiomers of 2-phosphosulfolactate. Able to hydrolyze both enantiomers of 2-hydroxycarboxylic acids with pseudosymmetric centers of inversion. Specifically hydrolyzes (S)-phospholactate and (S)-phosphoglycerate. HAMAP MF_00490

Catalytic activity

(2R)-2-phospho-3-sulfolactate + H2O = (2R)-3-sulfolactate + phosphate. HAMAP MF_00490

Cofactor

Magnesium. HAMAP MF_00490

Enzyme regulation

Inhibited by vanadate. HAMAP MF_00490

Pathway

Cofactor biosynthesis; coenzyme M biosynthesis; sulfoacetaldehyde from PEP and sulfite: step 2/4. HAMAP MF_00490

Subunit structure

Monomer. HAMAP MF_00490

Sequence similarities

Belongs to the comB family.

Biophysicochemical properties

pH dependence:

Optimum pH is 5.5. HAMAP MF_00490

Temperature dependence:

Optimum temperature is 75 degrees Celsius.

Ontologies

Keywords
   Biological processCoenzyme M biosynthesis
   LigandMagnesium
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcoenzyme M biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular function2-phosphosulfolactate phosphatase activity

Inferred from electronic annotation. Source: HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2242242-phosphosulfolactate phosphatase HAMAP MF_00490
PRO_0000081459

Sequences

Sequence LengthMass (Da)Tools
Q58540-1 [UniParc].

Last modified December 5, 2001. Version 2.
Checksum: B981C5B5635404E9

FASTA22425,114
        10         20         30         40         50         60 
MITLCNRFTE YKCGNVAIVV DVLRASTTIT TLLSFIDEVY ITTSTSKKEN AIYIGERKGR 

        70         80         90        100        110        120 
KIEGFDFGNS PTEILANKDI IKERYENGEK VILTTTNGTR VLKSLDAEHI FIGAIVNAKY 

       130        140        150        160        170        180 
VAKAVEDFED VSLVPCHREN NFAIDDFIGC GVIAKYLNGE FDEFIKAALE LTKHDWMSLI 

       190        200        210        220 
LNSSSAENLK NLGYEKDVTF AILENSIDAV GIYKKDKSKV VRFK 

« Hide

Cross-references

Sequence databases

L77117 Genomic DNA. Translation: AAB99140.1. Different initiation.
PIRC64442.
RefSeqNP_248132.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID1452036.
GenomeReviewsGene locus MJ1140 in contig L77117_GR.
KEGGmja:MJ1140.
NMPDRfig|243232.1.peg.1169.
TIGRMJ1140.

Phylogenomic databases

HOGENOMQ58540.

Enzyme and pathway databases

BioCycMetaCyc:MON-2263.
MJAN243232:MJ_1140-MON.
BRENDA3.1.3.71. 256362.

Family and domain databases

HAMAPMF_00490.
[Tree]
InterProIPR005238. 2-PSlactate_phosphatase.
[Graphical view]
Gene3DG3DSA:3.90.1560.10. 2-PSlactate_phosphatase. 1 hit.
PfamPF04029. 2-ph_phosp. 1 hit.
[Graphical view]
TIGRFAMsTIGR00298. 2-ph_phosphatase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCOMB_METJA
AccessionPrimary (citable) accession number: Q58540
Entry history
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: December 5, 2001
Last modified: June 16, 2009
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents