Q58530 (KAE1B_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Including the following 2 domains:

1. Probable tRNA threonylcarbamoyladenosine biosynthesis protein KAE1 homolog
   Alternative name(s):
   t(6)A37 threonylcarbamoyladenosine biosynthesis protein KAE1 homolog
2. Probable serine/threonine-protein kinase BUD32 homolog
   EC=2.7.11.1

Gene names

Ordered Locus Names:

MJ1130

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine By similarity. In vitro, exhibits low ATPase activity, but does not bind DNA and does not have endonuclease activity. Ref.4
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. HAMAP-Rule MF_01447
Enzyme regulation	The Kae1 region could inhibit the kinase activity of the C-terminal domain. Ref.2 Ref.3
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01447.
Sequence similarities	In the N-terminal section; belongs to the KAE1 / YgjD family. In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	ATP-binding Iron Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 535

535

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein HAMAP-Rule MF_01447

PRO_0000096979

Regions

Domain

333 – 535

203

Protein kinase

Nucleotide binding

339 – 347

9

ATP By similarity

Region

1 – 323

323

Kae1 HAMAP-Rule MF_01447

Sites

Active site

451

1

Proton acceptor By similarity

Metal binding

106

1

Iron By similarity

Metal binding

110

1

Iron By similarity

Metal binding

127

1

Iron By similarity

Metal binding

284

1

Iron By similarity

Binding site

360

1

ATP By similarity

Secondary structure

1

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535

Helix Strand Turn

Details...

Beta strand

2 – 7

6

Beta strand

9 – 19

11

Beta strand

24 – 31

8

Beta strand

37 – 40

4

Helix

44 – 62

19

Helix

65 – 67

3

Beta strand

70 – 78

9

Helix

80 – 97

18

Beta strand

101 – 105

5

Helix

106 – 117

12

Beta strand

124 – 128

5

Beta strand

133 – 139

7

Beta strand

142 – 152

11

Helix

154 – 164

11

Helix

172 – 180

9

Helix

200 – 211

12

Helix

216 – 242

27

Beta strand

245 – 251

7

Helix

252 – 255

4

Helix

257 – 269

13

Beta strand

273 – 275

3

Helix

279 – 282

4

Helix

286 – 298

13

Helix

305 – 307

3

Beta strand

345 – 351

7

Beta strand

356 – 362

7

Helix

370 – 390

21

Helix

391 – 394

4

Beta strand

401 – 405

5

Turn

406 – 409

4

Beta strand

410 – 414

5

Beta strand

418 – 420

3

Helix

421 – 424

4

Helix

430 – 444

15

Beta strand

456 – 465

10

Helix

477 – 494

18

Helix

496 – 498

3

Helix

499 – 513

15

Helix

517 – 528

12

Sequences

	Sequence		Length	Mass (Da)
	Q58530 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: B18EEFACE8E5A99F	FASTA	535	60,570
	10 20 30 40 50 60 MICLGLEGTA EKTGVGIVTS DGEVLFNKTI MYKPPKQGIN PREAADHHAE TFPKLIKEAF 70 80 90 100 110 120 EVVDKNEIDL IAFSQGPGLG PSLRVTATVA RTLSLTLKKP IIGVNHCIAH IEIGKLTTEA 130 140 150 160 170 180 EDPLTLYVSG GNTQVIAYVS KKYRVFGETL DIAVGNCLDQ FARYVNLPHP GGPYIEELAR 190 200 210 220 230 240 KGKKLVDLPY TVKGMDIAFS GLLTAAMRAY DAGERLEDIC YSLQEYAFSM LTEITERALA 250 260 270 280 290 300 HTNKGEVMLV GGVAANNRLR EMLKAMCEGQ NVDFYVPPKE FCGDNGAMIA WLGLLMHKNG 310 320 330 340 350 360 RWMSLDETKI IPNYRTDMVE VNWIKEIKGK KRKIPEHLIG KGAEADIKRD SYLDFDVIIK 370 380 390 400 410 420 ERVKKGYRDE RLDENIRKSR TAREARYLAL VKDFGIPAPY IFDVDLDNKR IMMSYINGKL 430 440 450 460 470 480 AKDVIEDNLD IAYKIGEIVG KLHKNDVIHN DLTTSNFIFD KDLYIIDFGL GKISNLDEDK 490 500 510 520 530 AVDLIVFKKA VLSTHHEKFD EIWERFLEGY KSVYDRWEII LELMKDVERR ARYVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"The universal Kae1 protein and the associated Bud32 kinase (PRPK), a mysterious protein couple probably essential for genome maintenance in Archaea and Eukarya." Hecker A., Graille M., Madec E., Gadelle D., Le Cam E., van Tilbergh H., Forterre P. Biochem. Soc. Trans. 37:29-35(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYME REGULATION.
[3]	"Structure of the archaeal Kae1/Bud32 fusion protein MJ1130: a model for the eukaryotic EKC/KEOPS subcomplex." Hecker A., Lopreiato R., Graille M., Collinet B., Forterre P., Libri D., van Tilbeurgh H. EMBO J. 27:2340-2351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS), ENZYME REGULATION.
[4]	"Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine." Mao D.Y., Neculai D., Downey M., Orlicky S., Haffani Y.Z., Ceccarelli D.F., Ho J.S., Szilard R.K., Zhang W., Ho C.S., Wan L., Fares C., Rumpel S., Kurinov I., Arrowsmith C.H., Durocher D., Sicheri F. Mol. Cell 32:259-275(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.67 ANGSTROMS), FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L77117 Genomic DNA. Translation: AAB99132.1.

RefSeq

NP_248122.1. NC_000909.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VWB	X-ray	3.05	A/B	1-535	[»]
3EN9	X-ray	2.67	A/B	1-535	[»]
3ENH	X-ray	3.60	A/B	1-535	[»]

ProteinModelPortal

Q58530.

ModBase

Search...

Protein-protein interaction databases

STRING

243232.MJ1130.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAB99132; AAB99132; MJ_1130.

GeneID

1452026.

KEGG

mja:MJ_1130.

Phylogenomic databases

eggNOG

COG0533.

KO

K15904.

OMA

GNCLDQF.

ProtClustDB

PRK09605.

Family and domain databases

HAMAP

MF_01447. Kae1_Bud32_arch.

InterPro

IPR000905. Gcp-like_dom.
IPR022495. Kae1-assoc_kinase_Bud32.
IPR017861. KAE1/YgjD.
IPR022449. Kae1_arc.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR000719. Prot_kinase_cat_dom.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]

PANTHER

PTHR11735:SF6. PTHR11735:SF6. 1 hit.

Pfam

PF00814. Peptidase_M22. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]

PIRSF

PIRSF036401. Gcp_STYKS. 1 hit.

PRINTS

PR00789. OSIALOPTASE.

SUPFAM

SSF56112. Kinase_like. 1 hit.

TIGRFAMs

TIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.

PROSITE

PS01016. GLYCOPROTEASE. 1 hit.
PS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q58530.

Entry information

Entry name

KAE1B_METJA

Accession

Primary (citable) accession number: Q58530

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	December 15, 1998
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program