ID   HELS_METJA              Reviewed;        1195 AA.
AC   Q58524;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 153.
DE   RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
DE            EC=5.6.2.4 {ECO:0000255|HAMAP-Rule:MF_00442};
DE   AltName: Full=DNA 3'-5' helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
DE   Contains:
DE     RecName: Full=Endonuclease PI-MjaHel;
DE              EC=3.1.-.-;
DE     AltName: Full=Mja Hel intein;
DE     AltName: Full=Mja Pep3 intein;
GN   Name=hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
GN   OrderedLocusNames=MJ1124;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC       involved in repair of stalled replication forks. {ECO:0000255|HAMAP-
CC       Rule:MF_00442}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC         translocating in the 3'-5' direction.; EC=5.6.2.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00442}.
CC   -!- PTM: This protein undergoes a protein self splicing that involves a
CC       post-translational excision of the intervening region (intein) followed
CC       by peptide ligation. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00442}.
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DR   EMBL; L77117; AAB99126.1; -; Genomic_DNA.
DR   PIR; C64440; C64440.
DR   RefSeq; WP_010870635.1; NC_000909.1.
DR   AlphaFoldDB; Q58524; -.
DR   SMR; Q58524; -.
DR   STRING; 243232.MJ_1124; -.
DR   PaxDb; 243232-MJ_1124; -.
DR   EnsemblBacteria; AAB99126; AAB99126; MJ_1124.
DR   GeneID; 1452020; -.
DR   KEGG; mja:MJ_1124; -.
DR   eggNOG; arCOG00553; Archaea.
DR   eggNOG; arCOG03157; Archaea.
DR   HOGENOM; CLU_279743_0_0_2; -.
DR   InParanoid; Q58524; -.
DR   OrthoDB; 371946at2157; -.
DR   PhylomeDB; Q58524; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR   GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR   CDD; cd18028; DEXHc_archSki2; 1.
DR   CDD; cd00081; Hint; 1.
DR   Gene3D; 1.10.3380.30; -; 1.
DR   Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR   Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 2.
DR   Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR   HAMAP; MF_00442; Helicase_Hel308; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR048772; Hel308-like_dom4.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR022965; Helicase_Hel308.
DR   InterPro; IPR003586; Hint_dom_C.
DR   InterPro; IPR003587; Hint_dom_N.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR006142; INTEIN.
DR   InterPro; IPR030934; Intein_C.
DR   InterPro; IPR004042; Intein_endonuc.
DR   InterPro; IPR006141; Intein_N.
DR   InterPro; IPR004860; LAGLIDADG_2.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   NCBIfam; TIGR01443; intein_Cterm; 1.
DR   NCBIfam; TIGR01445; intein_Nterm; 1.
DR   PANTHER; PTHR47961:SF10; ATP-DEPENDENT DNA HELICASE HEL308; 1.
DR   PANTHER; PTHR47961; DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED; 1.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF21280; Helicase_dom4_arc; 1.
DR   Pfam; PF14520; HHH_5; 1.
DR   Pfam; PF14890; Intein_splicing; 1.
DR   Pfam; PF14528; LAGLIDADG_3; 1.
DR   PRINTS; PR00379; INTEIN.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00305; HintC; 1.
DR   SMART; SM00306; HintN; 1.
DR   SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR   SUPFAM; SSF55608; Homing endonucleases; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 3.
DR   SUPFAM; SSF158702; Sec63 N-terminal domain-like; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS50818; INTEIN_C_TER; 1.
DR   PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR   PROSITE; PS50817; INTEIN_N_TER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Autocatalytic cleavage; DNA damage; DNA repair; DNA-binding;
KW   Endonuclease; Helicase; Hydrolase; Intron homing; Isomerase; Nuclease;
KW   Nucleotide-binding; Protein splicing; Reference proteome.
FT   CHAIN           1..337
FT                   /note="ATP-dependent DNA helicase Hel308, 1st part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013299"
FT   CHAIN           338..838
FT                   /note="Endonuclease PI-MjaHel"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013300"
FT   CHAIN           839..1195
FT                   /note="ATP-dependent DNA helicase Hel308, 2nd part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013301"
FT   DOMAIN          26..196
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   DOMAIN          451..584
FT                   /note="DOD-type homing endonuclease"
FT   MOTIF           143..146
FT                   /note="DEAH box"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   BINDING         20
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   BINDING         39..46
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
SQ   SEQUENCE   1195 AA;  138674 MW;  87C9CF1A87EFB52D CRC64;
     MDKILEILKD FGIVELRPPQ KKALERGLLD KNKNFLISIP TASGKTLIGE MALINHLLDG
     NKNPTNKKGI FIVPLKALAS EKYEEFKSKY ERYGLRIALS IGDYDEDEDL SKYHLIITTA
     EKLDSLWRHK IDWINDVSVV VVDEIHLIND ETRGGTLEIL LTKLKEFNVQ IIGLSATIGN
     PDELAEWLNA ELIVDDWRPV ELKKGIYKNE AIEFINGEIR EIKAVDNNDI YNLVVDCVKE
     GGCCLVFCNT KRNAVNEAKK LNLKKFLTEE EKIRLKEIAE EILSILEPPT EMCKTLAECI
     LNGSAFHHAG LTYQHRKIVE DAFRKRLIKV ICCTPTLCLN ANTEILQESG FRKITELNKD
     EKVFALCGKE IKPVDGWKVH KTPQHEYNIV VKTVNGLEIT TTPNHIFLVK ENGSLKEKEA
     KDLKVGDYVA TVDRIRVKEK DIDLSNGDLY FIGYFIGDGY TGVIEKNTLK ATPDLAFNPK
     YPPNFDDSEL HKKYFLKCRI SKGVAHYIYS KKLRKIFNKL NMLTKDNKNI DAFCNLPLDK
     LAYLIAGLFD SDGYIYLNRK NIEFYSISEK LVEQLQFVLL RFGIHSSIRK KKTKTMVSPT
     NGKEYKCKDI YVLTIRDFMS IKRFYENIPL RHEEKRRKLE EIIKNKEIGQ IPSEFVALRF
     TPIAKIWCDC GFSVDLTMFK PRTKRQRELN KKRVKLLFEL LDGKKLITNY KEYYSKRKNP
     YFDFIVREKI NGNNYYSLNE KGRVLMSLLN KHIKDKENLE EMYNFLVNLE KCPICGKPIH
     KEMRYSWKKE CYDGDIYWDR IKEIKKIKVN DKYAYDIELP DDGSNSHYIV ANGFIVHNSA
     GLNLPCRRAI VKDLTRFTNK GMRYIPIMEI QQCIGRAGRP GLDPYGEGII VAKNDRDYLR
     AYQALTQKPE PIYSKLSNQA VLRTQLLGLI ATGEIRDEYD LEWFIRNTFY AHQYGNLREV
     AKNINEVIRF LEENEFIIDF MPTELGKRVS ELYIDPLSAK FIIDGLEEME NEEEIYYLYL
     ISKTLEMMPN LRVYNSEELN LIDEMDSLGI KSFEIEDLEA FKTAKMLYDW INEVPEDEIL
     KRYKIEPGIL RYKVENAVWI MHALKEIAKL IGKSSDIPEK LEIRLEYGAK EDIIELLSIK
     YIGRVRARKL YNAGIRSIED IINNPSKVAS IIGEKIAKKI LDELGVKFGQ QKLSF
//