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Q58524

- HELS_METJA

UniProt

Q58524 - HELS_METJA

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Protein
ATP-dependent DNA helicase Hel308
Gene
hel308, MJ1124
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks By similarity.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi39 – 468ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. endonuclease activity Source: UniProtKB-KW
  4. helicase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA repair Source: UniProtKB-HAMAP
  2. intein-mediated protein splicing Source: InterPro
  3. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair, Intron homing

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase Hel308 (EC:3.6.4.12)
Cleaved into the following chain:
Alternative name(s):
Mja Hel intein
Mja Pep3 intein
Gene namesi
Name:hel308
Ordered Locus Names:MJ1124
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337ATP-dependent DNA helicase Hel308, 1st part Reviewed prediction
PRO_0000013299Add
BLAST
Chaini338 – 838501Endonuclease PI-MjaHel Reviewed prediction
PRO_0000013300Add
BLAST
Chaini839 – 1195357ATP-dependent DNA helicase Hel308, 2nd part Reviewed prediction
PRO_0000013301Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Reviewed prediction.UniRule annotation

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi243232.MJ1124.

Structurei

3D structure databases

ProteinModelPortaliQ58524.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 196171Helicase ATP-binding
Add
BLAST
Domaini451 – 584134DOD-type homing endonuclease
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi143 – 1464DEAH boxUniRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi137 – 1426Poly-ValUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1372.
KOiK03726.
OMAiCLNANTE.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 5 hits.
HAMAPiMF_00442. Helicase_Hel308.
InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR028992. Hedgehog/Intein_dom.
IPR014001. Helicase_ATP-bd.
IPR022965. Helicase_ski2.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00487. DEXDc. 1 hit.
SM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58524-1 [UniParc]FASTAAdd to Basket

« Hide

MDKILEILKD FGIVELRPPQ KKALERGLLD KNKNFLISIP TASGKTLIGE     50
MALINHLLDG NKNPTNKKGI FIVPLKALAS EKYEEFKSKY ERYGLRIALS 100
IGDYDEDEDL SKYHLIITTA EKLDSLWRHK IDWINDVSVV VVDEIHLIND 150
ETRGGTLEIL LTKLKEFNVQ IIGLSATIGN PDELAEWLNA ELIVDDWRPV 200
ELKKGIYKNE AIEFINGEIR EIKAVDNNDI YNLVVDCVKE GGCCLVFCNT 250
KRNAVNEAKK LNLKKFLTEE EKIRLKEIAE EILSILEPPT EMCKTLAECI 300
LNGSAFHHAG LTYQHRKIVE DAFRKRLIKV ICCTPTLCLN ANTEILQESG 350
FRKITELNKD EKVFALCGKE IKPVDGWKVH KTPQHEYNIV VKTVNGLEIT 400
TTPNHIFLVK ENGSLKEKEA KDLKVGDYVA TVDRIRVKEK DIDLSNGDLY 450
FIGYFIGDGY TGVIEKNTLK ATPDLAFNPK YPPNFDDSEL HKKYFLKCRI 500
SKGVAHYIYS KKLRKIFNKL NMLTKDNKNI DAFCNLPLDK LAYLIAGLFD 550
SDGYIYLNRK NIEFYSISEK LVEQLQFVLL RFGIHSSIRK KKTKTMVSPT 600
NGKEYKCKDI YVLTIRDFMS IKRFYENIPL RHEEKRRKLE EIIKNKEIGQ 650
IPSEFVALRF TPIAKIWCDC GFSVDLTMFK PRTKRQRELN KKRVKLLFEL 700
LDGKKLITNY KEYYSKRKNP YFDFIVREKI NGNNYYSLNE KGRVLMSLLN 750
KHIKDKENLE EMYNFLVNLE KCPICGKPIH KEMRYSWKKE CYDGDIYWDR 800
IKEIKKIKVN DKYAYDIELP DDGSNSHYIV ANGFIVHNSA GLNLPCRRAI 850
VKDLTRFTNK GMRYIPIMEI QQCIGRAGRP GLDPYGEGII VAKNDRDYLR 900
AYQALTQKPE PIYSKLSNQA VLRTQLLGLI ATGEIRDEYD LEWFIRNTFY 950
AHQYGNLREV AKNINEVIRF LEENEFIIDF MPTELGKRVS ELYIDPLSAK 1000
FIIDGLEEME NEEEIYYLYL ISKTLEMMPN LRVYNSEELN LIDEMDSLGI 1050
KSFEIEDLEA FKTAKMLYDW INEVPEDEIL KRYKIEPGIL RYKVENAVWI 1100
MHALKEIAKL IGKSSDIPEK LEIRLEYGAK EDIIELLSIK YIGRVRARKL 1150
YNAGIRSIED IINNPSKVAS IIGEKIAKKI LDELGVKFGQ QKLSF 1195
Length:1,195
Mass (Da):138,674
Last modified:November 1, 1996 - v1
Checksum:i87C9CF1A87EFB52D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99126.1.
PIRiC64440.
RefSeqiNP_248116.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99126; AAB99126; MJ_1124.
GeneIDi1452020.
KEGGimja:MJ_1124.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99126.1 .
PIRi C64440.
RefSeqi NP_248116.1. NC_000909.1.

3D structure databases

ProteinModelPortali Q58524.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJ1124.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB99126 ; AAB99126 ; MJ_1124 .
GeneIDi 1452020.
KEGGi mja:MJ_1124.

Phylogenomic databases

eggNOGi COG1372.
KOi K03726.
OMAi CLNANTE.

Family and domain databases

Gene3Di 2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 5 hits.
HAMAPi MF_00442. Helicase_Hel308.
InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR028992. Hedgehog/Intein_dom.
IPR014001. Helicase_ATP-bd.
IPR022965. Helicase_ski2.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00487. DEXDc. 1 hit.
SM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiHELS_METJA
AccessioniPrimary (citable) accession number: Q58524
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3