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Q58524

- HELS_METJA

UniProt

Q58524 - HELS_METJA

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Protein

ATP-dependent DNA helicase Hel308

Gene

hel308

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi39 – 468ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. endonuclease activity Source: UniProtKB-KW
  4. helicase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA repair Source: UniProtKB-HAMAP
  2. intein-mediated protein splicing Source: InterPro
  3. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair, Intron homing

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase Hel308UniRule annotation (EC:3.6.4.12UniRule annotation)
Cleaved into the following chain:
Alternative name(s):
Mja Hel intein
Mja Pep3 intein
Gene namesi
Name:hel308UniRule annotation
Ordered Locus Names:MJ1124
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337ATP-dependent DNA helicase Hel308, 1st partSequence AnalysisPRO_0000013299Add
BLAST
Chaini338 – 838501Endonuclease PI-MjaHelSequence AnalysisPRO_0000013300Add
BLAST
Chaini839 – 1195357ATP-dependent DNA helicase Hel308, 2nd partSequence AnalysisPRO_0000013301Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243232.MJ1124.

Structurei

3D structure databases

ProteinModelPortaliQ58524.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 196171Helicase ATP-bindingUniRule annotationAdd
BLAST
Domaini451 – 584134DOD-type homing endonucleaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi143 – 1464DEAH box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi137 – 1426Poly-Val

Sequence similaritiesi

Belongs to the helicase family. Hel308 subfamily.UniRule annotation
Contains 1 helicase ATP-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1372.
InParanoidiQ58524.
KOiK03726.
OMAiCLNANTE.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 5 hits.
HAMAPiMF_00442. Helicase_Hel308.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR028992. Hedgehog/Intein_dom.
IPR014001. Helicase_ATP-bd.
IPR022965. Helicase_ski2.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00487. DEXDc. 1 hit.
SM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58524-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDKILEILKD FGIVELRPPQ KKALERGLLD KNKNFLISIP TASGKTLIGE
60 70 80 90 100
MALINHLLDG NKNPTNKKGI FIVPLKALAS EKYEEFKSKY ERYGLRIALS
110 120 130 140 150
IGDYDEDEDL SKYHLIITTA EKLDSLWRHK IDWINDVSVV VVDEIHLIND
160 170 180 190 200
ETRGGTLEIL LTKLKEFNVQ IIGLSATIGN PDELAEWLNA ELIVDDWRPV
210 220 230 240 250
ELKKGIYKNE AIEFINGEIR EIKAVDNNDI YNLVVDCVKE GGCCLVFCNT
260 270 280 290 300
KRNAVNEAKK LNLKKFLTEE EKIRLKEIAE EILSILEPPT EMCKTLAECI
310 320 330 340 350
LNGSAFHHAG LTYQHRKIVE DAFRKRLIKV ICCTPTLCLN ANTEILQESG
360 370 380 390 400
FRKITELNKD EKVFALCGKE IKPVDGWKVH KTPQHEYNIV VKTVNGLEIT
410 420 430 440 450
TTPNHIFLVK ENGSLKEKEA KDLKVGDYVA TVDRIRVKEK DIDLSNGDLY
460 470 480 490 500
FIGYFIGDGY TGVIEKNTLK ATPDLAFNPK YPPNFDDSEL HKKYFLKCRI
510 520 530 540 550
SKGVAHYIYS KKLRKIFNKL NMLTKDNKNI DAFCNLPLDK LAYLIAGLFD
560 570 580 590 600
SDGYIYLNRK NIEFYSISEK LVEQLQFVLL RFGIHSSIRK KKTKTMVSPT
610 620 630 640 650
NGKEYKCKDI YVLTIRDFMS IKRFYENIPL RHEEKRRKLE EIIKNKEIGQ
660 670 680 690 700
IPSEFVALRF TPIAKIWCDC GFSVDLTMFK PRTKRQRELN KKRVKLLFEL
710 720 730 740 750
LDGKKLITNY KEYYSKRKNP YFDFIVREKI NGNNYYSLNE KGRVLMSLLN
760 770 780 790 800
KHIKDKENLE EMYNFLVNLE KCPICGKPIH KEMRYSWKKE CYDGDIYWDR
810 820 830 840 850
IKEIKKIKVN DKYAYDIELP DDGSNSHYIV ANGFIVHNSA GLNLPCRRAI
860 870 880 890 900
VKDLTRFTNK GMRYIPIMEI QQCIGRAGRP GLDPYGEGII VAKNDRDYLR
910 920 930 940 950
AYQALTQKPE PIYSKLSNQA VLRTQLLGLI ATGEIRDEYD LEWFIRNTFY
960 970 980 990 1000
AHQYGNLREV AKNINEVIRF LEENEFIIDF MPTELGKRVS ELYIDPLSAK
1010 1020 1030 1040 1050
FIIDGLEEME NEEEIYYLYL ISKTLEMMPN LRVYNSEELN LIDEMDSLGI
1060 1070 1080 1090 1100
KSFEIEDLEA FKTAKMLYDW INEVPEDEIL KRYKIEPGIL RYKVENAVWI
1110 1120 1130 1140 1150
MHALKEIAKL IGKSSDIPEK LEIRLEYGAK EDIIELLSIK YIGRVRARKL
1160 1170 1180 1190
YNAGIRSIED IINNPSKVAS IIGEKIAKKI LDELGVKFGQ QKLSF
Length:1,195
Mass (Da):138,674
Last modified:November 1, 1996 - v1
Checksum:i87C9CF1A87EFB52D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99126.1.
PIRiC64440.
RefSeqiNP_248116.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99126; AAB99126; MJ_1124.
GeneIDi1452020.
KEGGimja:MJ_1124.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99126.1 .
PIRi C64440.
RefSeqi NP_248116.1. NC_000909.1.

3D structure databases

ProteinModelPortali Q58524.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJ1124.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB99126 ; AAB99126 ; MJ_1124 .
GeneIDi 1452020.
KEGGi mja:MJ_1124.

Phylogenomic databases

eggNOGi COG1372.
InParanoidi Q58524.
KOi K03726.
OMAi CLNANTE.

Family and domain databases

Gene3Di 2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 5 hits.
HAMAPi MF_00442. Helicase_Hel308.
InterProi IPR011545. DEAD/DEAH_box_helicase_dom.
IPR028992. Hedgehog/Intein_dom.
IPR014001. Helicase_ATP-bd.
IPR022965. Helicase_ski2.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00487. DEXDc. 1 hit.
SM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiHELS_METJA
AccessioniPrimary (citable) accession number: Q58524
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3