Putative ski2-type helicase - Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Putative ski2-type helicase
EC=3.6.4.-

Cleaved into the following chain:

Endonuclease PI-MjaHel
EC=3.1.-.-
Alternative name(s):
Mja Hel intein
Mja Pep3 intein

Gene names

Ordered Locus Names:

MJ1124

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	1195 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP MF_00442

Sequence similarities

Belongs to the helicase family. SKI2 subfamily.

Contains 1 DOD-type homing endonuclease domain.

Contains 1 helicase ATP-binding domain.

Ontologies

Keywords
Biological process	Intron homing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Endonuclease Helicase Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 337

337

Putative ski2-type helicase, 1st part Potential

PRO_0000013299

Chain

338 – 838

501

Endonuclease PI-MjaHel Potential

PRO_0000013300

Chain

839 – 1195

357

Putative ski2-type helicase, 2nd part Potential

PRO_0000013301

Regions

Domain

26 – 196

171

Helicase ATP-binding

Domain

451 – 584

134

DOD-type homing endonuclease

Nucleotide binding

39 – 46

8

ATP By similarity

Motif

143 – 146

4

DEAH box HAMAP MF_00442

Compositional bias

137 – 142

6

Poly-Val HAMAP MF_00442

Sequences

Sequence

Length

Mass (Da)

Tools

Q58524 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 87C9CF1A87EFB52D
Show »

FASTA

1,195

138,674

        10         20         30         40         50         60 
MDKILEILKD FGIVELRPPQ KKALERGLLD KNKNFLISIP TASGKTLIGE MALINHLLDG 

        70         80         90        100        110        120 
NKNPTNKKGI FIVPLKALAS EKYEEFKSKY ERYGLRIALS IGDYDEDEDL SKYHLIITTA 

       130        140        150        160        170        180 
EKLDSLWRHK IDWINDVSVV VVDEIHLIND ETRGGTLEIL LTKLKEFNVQ IIGLSATIGN 

       190        200        210        220        230        240 
PDELAEWLNA ELIVDDWRPV ELKKGIYKNE AIEFINGEIR EIKAVDNNDI YNLVVDCVKE 

       250        260        270        280        290        300 
GGCCLVFCNT KRNAVNEAKK LNLKKFLTEE EKIRLKEIAE EILSILEPPT EMCKTLAECI 

       310        320        330        340        350        360 
LNGSAFHHAG LTYQHRKIVE DAFRKRLIKV ICCTPTLCLN ANTEILQESG FRKITELNKD 

       370        380        390        400        410        420 
EKVFALCGKE IKPVDGWKVH KTPQHEYNIV VKTVNGLEIT TTPNHIFLVK ENGSLKEKEA 

       430        440        450        460        470        480 
KDLKVGDYVA TVDRIRVKEK DIDLSNGDLY FIGYFIGDGY TGVIEKNTLK ATPDLAFNPK 

       490        500        510        520        530        540 
YPPNFDDSEL HKKYFLKCRI SKGVAHYIYS KKLRKIFNKL NMLTKDNKNI DAFCNLPLDK 

       550        560        570        580        590        600 
LAYLIAGLFD SDGYIYLNRK NIEFYSISEK LVEQLQFVLL RFGIHSSIRK KKTKTMVSPT 

       610        620        630        640        650        660 
NGKEYKCKDI YVLTIRDFMS IKRFYENIPL RHEEKRRKLE EIIKNKEIGQ IPSEFVALRF 

       670        680        690        700        710        720 
TPIAKIWCDC GFSVDLTMFK PRTKRQRELN KKRVKLLFEL LDGKKLITNY KEYYSKRKNP 

       730        740        750        760        770        780 
YFDFIVREKI NGNNYYSLNE KGRVLMSLLN KHIKDKENLE EMYNFLVNLE KCPICGKPIH 

       790        800        810        820        830        840 
KEMRYSWKKE CYDGDIYWDR IKEIKKIKVN DKYAYDIELP DDGSNSHYIV ANGFIVHNSA 

       850        860        870        880        890        900 
GLNLPCRRAI VKDLTRFTNK GMRYIPIMEI QQCIGRAGRP GLDPYGEGII VAKNDRDYLR 

       910        920        930        940        950        960 
AYQALTQKPE PIYSKLSNQA VLRTQLLGLI ATGEIRDEYD LEWFIRNTFY AHQYGNLREV 

       970        980        990       1000       1010       1020 
AKNINEVIRF LEENEFIIDF MPTELGKRVS ELYIDPLSAK FIIDGLEEME NEEEIYYLYL 

      1030       1040       1050       1060       1070       1080 
ISKTLEMMPN LRVYNSEELN LIDEMDSLGI KSFEIEDLEA FKTAKMLYDW INEVPEDEIL 

      1090       1100       1110       1120       1130       1140 
KRYKIEPGIL RYKVENAVWI MHALKEIAKL IGKSSDIPEK LEIRLEYGAK EDIIELLSIK 

      1150       1160       1170       1180       1190 
YIGRVRARKL YNAGIRSIED IINNPSKVAS IIGEKIAKKI LDELGVKFGQ QKLSF

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References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii."
Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G.

Venter J.C.
Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99126.1.
PIR	C64440.
RefSeq	NP_248116.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58524.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1452020.
GenomeReviews	Gene locus MJ1124 in contig L77117_GR.
KEGG	mja:MJ_1124.
NMPDR	fig\|243232.1.peg.1153.
TIGR	MJ1124.
Phylogenomic databases
HOGENOM	HBG541258.
OMA	CLNANTE.
ProtClustDB	CLSK796742.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1124-MONOMER.
Family and domain databases
HAMAP	MF_00442. Helicase_ski2. [Tree]
InterPro	IPR014001. DEAD-like_helicase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. [Graphical view]
KO	K03726.
Pfam	PF00270. DEAD. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00487. DEXDc. 1 hit. SM00278. HhH1. 2 hits. SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. Intein_Cterm. 1 hit. TIGR01445. Intein_Nterm. 1 hit.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HELS_METJA

Accession

Primary (citable) accession number: Q58524

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Cleaved into the following chain:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents