Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent DNA helicase Hel308

Gene

hel308

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 46ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair, Intron homing

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase Hel308UniRule annotation (EC:3.6.4.12UniRule annotation)
Cleaved into the following chain:
Alternative name(s):
Mja Hel intein
Mja Pep3 intein
Gene namesi
Name:hel308UniRule annotation
Ordered Locus Names:MJ1124
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000132991 – 337ATP-dependent DNA helicase Hel308, 1st partSequence analysisAdd BLAST337
ChainiPRO_0000013300338 – 838Endonuclease PI-MjaHelSequence analysisAdd BLAST501
ChainiPRO_0000013301839 – 1195ATP-dependent DNA helicase Hel308, 2nd partSequence analysisAdd BLAST357

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243232.MJ_1124.

Structurei

3D structure databases

ProteinModelPortaliQ58524.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 196Helicase ATP-bindingUniRule annotationAdd BLAST171
Domaini451 – 584DOD-type homing endonucleaseAdd BLAST134

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi143 – 146DEAH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi137 – 142Poly-Val6

Sequence similaritiesi

Belongs to the helicase family. Hel308 subfamily.UniRule annotation
Contains 1 helicase ATP-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00553. Archaea.
arCOG03157. Archaea.
COG1204. LUCA.
COG1372. LUCA.
InParanoidiQ58524.
KOiK03726.
OMAiCLNANTE.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 5 hits.
HAMAPiMF_00442. Helicase_Hel308. 1 hit.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR028992. Hedgehog/Intein_dom.
IPR014001. Helicase_ATP-bd.
IPR022965. Helicase_Hel308.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00487. DEXDc. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58524-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKILEILKD FGIVELRPPQ KKALERGLLD KNKNFLISIP TASGKTLIGE
60 70 80 90 100
MALINHLLDG NKNPTNKKGI FIVPLKALAS EKYEEFKSKY ERYGLRIALS
110 120 130 140 150
IGDYDEDEDL SKYHLIITTA EKLDSLWRHK IDWINDVSVV VVDEIHLIND
160 170 180 190 200
ETRGGTLEIL LTKLKEFNVQ IIGLSATIGN PDELAEWLNA ELIVDDWRPV
210 220 230 240 250
ELKKGIYKNE AIEFINGEIR EIKAVDNNDI YNLVVDCVKE GGCCLVFCNT
260 270 280 290 300
KRNAVNEAKK LNLKKFLTEE EKIRLKEIAE EILSILEPPT EMCKTLAECI
310 320 330 340 350
LNGSAFHHAG LTYQHRKIVE DAFRKRLIKV ICCTPTLCLN ANTEILQESG
360 370 380 390 400
FRKITELNKD EKVFALCGKE IKPVDGWKVH KTPQHEYNIV VKTVNGLEIT
410 420 430 440 450
TTPNHIFLVK ENGSLKEKEA KDLKVGDYVA TVDRIRVKEK DIDLSNGDLY
460 470 480 490 500
FIGYFIGDGY TGVIEKNTLK ATPDLAFNPK YPPNFDDSEL HKKYFLKCRI
510 520 530 540 550
SKGVAHYIYS KKLRKIFNKL NMLTKDNKNI DAFCNLPLDK LAYLIAGLFD
560 570 580 590 600
SDGYIYLNRK NIEFYSISEK LVEQLQFVLL RFGIHSSIRK KKTKTMVSPT
610 620 630 640 650
NGKEYKCKDI YVLTIRDFMS IKRFYENIPL RHEEKRRKLE EIIKNKEIGQ
660 670 680 690 700
IPSEFVALRF TPIAKIWCDC GFSVDLTMFK PRTKRQRELN KKRVKLLFEL
710 720 730 740 750
LDGKKLITNY KEYYSKRKNP YFDFIVREKI NGNNYYSLNE KGRVLMSLLN
760 770 780 790 800
KHIKDKENLE EMYNFLVNLE KCPICGKPIH KEMRYSWKKE CYDGDIYWDR
810 820 830 840 850
IKEIKKIKVN DKYAYDIELP DDGSNSHYIV ANGFIVHNSA GLNLPCRRAI
860 870 880 890 900
VKDLTRFTNK GMRYIPIMEI QQCIGRAGRP GLDPYGEGII VAKNDRDYLR
910 920 930 940 950
AYQALTQKPE PIYSKLSNQA VLRTQLLGLI ATGEIRDEYD LEWFIRNTFY
960 970 980 990 1000
AHQYGNLREV AKNINEVIRF LEENEFIIDF MPTELGKRVS ELYIDPLSAK
1010 1020 1030 1040 1050
FIIDGLEEME NEEEIYYLYL ISKTLEMMPN LRVYNSEELN LIDEMDSLGI
1060 1070 1080 1090 1100
KSFEIEDLEA FKTAKMLYDW INEVPEDEIL KRYKIEPGIL RYKVENAVWI
1110 1120 1130 1140 1150
MHALKEIAKL IGKSSDIPEK LEIRLEYGAK EDIIELLSIK YIGRVRARKL
1160 1170 1180 1190
YNAGIRSIED IINNPSKVAS IIGEKIAKKI LDELGVKFGQ QKLSF
Length:1,195
Mass (Da):138,674
Last modified:November 1, 1996 - v1
Checksum:i87C9CF1A87EFB52D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99126.1.
PIRiC64440.
RefSeqiWP_010870635.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99126; AAB99126; MJ_1124.
GeneIDi1452020.
KEGGimja:MJ_1124.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99126.1.
PIRiC64440.
RefSeqiWP_010870635.1. NC_000909.1.

3D structure databases

ProteinModelPortaliQ58524.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_1124.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99126; AAB99126; MJ_1124.
GeneIDi1452020.
KEGGimja:MJ_1124.

Phylogenomic databases

eggNOGiarCOG00553. Archaea.
arCOG03157. Archaea.
COG1204. LUCA.
COG1372. LUCA.
InParanoidiQ58524.
KOiK03726.
OMAiCLNANTE.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 5 hits.
HAMAPiMF_00442. Helicase_Hel308. 1 hit.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR028992. Hedgehog/Intein_dom.
IPR014001. Helicase_ATP-bd.
IPR022965. Helicase_Hel308.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00487. DEXDc. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHELS_METJA
AccessioniPrimary (citable) accession number: Q58524
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.