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Q58524

- HELS_METJA

UniProt

Q58524 - HELS_METJA

Protein

ATP-dependent DNA helicase Hel308

Gene

hel308

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks.UniRule annotation

    Catalytic activityi

    ATP + H2O = ADP + phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei20 – 201ATPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi39 – 468ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. DNA binding Source: UniProtKB-HAMAP
    3. endonuclease activity Source: UniProtKB-KW
    4. helicase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA repair Source: UniProtKB-HAMAP
    2. intein-mediated protein splicing Source: InterPro
    3. intron homing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Helicase, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA repair, Intron homing

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent DNA helicase Hel308UniRule annotation (EC:3.6.4.12UniRule annotation)
    Cleaved into the following chain:
    Alternative name(s):
    Mja Hel intein
    Mja Pep3 intein
    Gene namesi
    Name:hel308UniRule annotation
    Ordered Locus Names:MJ1124
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 337337ATP-dependent DNA helicase Hel308, 1st partSequence AnalysisPRO_0000013299Add
    BLAST
    Chaini338 – 838501Endonuclease PI-MjaHelSequence AnalysisPRO_0000013300Add
    BLAST
    Chaini839 – 1195357ATP-dependent DNA helicase Hel308, 2nd partSequence AnalysisPRO_0000013301Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi243232.MJ1124.

    Structurei

    3D structure databases

    ProteinModelPortaliQ58524.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 196171Helicase ATP-bindingUniRule annotationAdd
    BLAST
    Domaini451 – 584134DOD-type homing endonucleaseAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi143 – 1464DEAH box

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi137 – 1426Poly-Val

    Sequence similaritiesi

    Belongs to the helicase family. Hel308 subfamily.UniRule annotation
    Contains 1 helicase ATP-binding domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1372.
    KOiK03726.
    OMAiCLNANTE.

    Family and domain databases

    Gene3Di2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.40.50.300. 5 hits.
    HAMAPiMF_00442. Helicase_Hel308.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR028992. Hedgehog/Intein_dom.
    IPR014001. Helicase_ATP-bd.
    IPR022965. Helicase_ski2.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00487. DEXDc. 1 hit.
    SM00278. HhH1. 2 hits.
    SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view]
    SUPFAMiSSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 3 hits.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q58524-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDKILEILKD FGIVELRPPQ KKALERGLLD KNKNFLISIP TASGKTLIGE     50
    MALINHLLDG NKNPTNKKGI FIVPLKALAS EKYEEFKSKY ERYGLRIALS 100
    IGDYDEDEDL SKYHLIITTA EKLDSLWRHK IDWINDVSVV VVDEIHLIND 150
    ETRGGTLEIL LTKLKEFNVQ IIGLSATIGN PDELAEWLNA ELIVDDWRPV 200
    ELKKGIYKNE AIEFINGEIR EIKAVDNNDI YNLVVDCVKE GGCCLVFCNT 250
    KRNAVNEAKK LNLKKFLTEE EKIRLKEIAE EILSILEPPT EMCKTLAECI 300
    LNGSAFHHAG LTYQHRKIVE DAFRKRLIKV ICCTPTLCLN ANTEILQESG 350
    FRKITELNKD EKVFALCGKE IKPVDGWKVH KTPQHEYNIV VKTVNGLEIT 400
    TTPNHIFLVK ENGSLKEKEA KDLKVGDYVA TVDRIRVKEK DIDLSNGDLY 450
    FIGYFIGDGY TGVIEKNTLK ATPDLAFNPK YPPNFDDSEL HKKYFLKCRI 500
    SKGVAHYIYS KKLRKIFNKL NMLTKDNKNI DAFCNLPLDK LAYLIAGLFD 550
    SDGYIYLNRK NIEFYSISEK LVEQLQFVLL RFGIHSSIRK KKTKTMVSPT 600
    NGKEYKCKDI YVLTIRDFMS IKRFYENIPL RHEEKRRKLE EIIKNKEIGQ 650
    IPSEFVALRF TPIAKIWCDC GFSVDLTMFK PRTKRQRELN KKRVKLLFEL 700
    LDGKKLITNY KEYYSKRKNP YFDFIVREKI NGNNYYSLNE KGRVLMSLLN 750
    KHIKDKENLE EMYNFLVNLE KCPICGKPIH KEMRYSWKKE CYDGDIYWDR 800
    IKEIKKIKVN DKYAYDIELP DDGSNSHYIV ANGFIVHNSA GLNLPCRRAI 850
    VKDLTRFTNK GMRYIPIMEI QQCIGRAGRP GLDPYGEGII VAKNDRDYLR 900
    AYQALTQKPE PIYSKLSNQA VLRTQLLGLI ATGEIRDEYD LEWFIRNTFY 950
    AHQYGNLREV AKNINEVIRF LEENEFIIDF MPTELGKRVS ELYIDPLSAK 1000
    FIIDGLEEME NEEEIYYLYL ISKTLEMMPN LRVYNSEELN LIDEMDSLGI 1050
    KSFEIEDLEA FKTAKMLYDW INEVPEDEIL KRYKIEPGIL RYKVENAVWI 1100
    MHALKEIAKL IGKSSDIPEK LEIRLEYGAK EDIIELLSIK YIGRVRARKL 1150
    YNAGIRSIED IINNPSKVAS IIGEKIAKKI LDELGVKFGQ QKLSF 1195
    Length:1,195
    Mass (Da):138,674
    Last modified:November 1, 1996 - v1
    Checksum:i87C9CF1A87EFB52D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99126.1.
    PIRiC64440.
    RefSeqiNP_248116.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB99126; AAB99126; MJ_1124.
    GeneIDi1452020.
    KEGGimja:MJ_1124.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99126.1 .
    PIRi C64440.
    RefSeqi NP_248116.1. NC_000909.1.

    3D structure databases

    ProteinModelPortali Q58524.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ1124.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB99126 ; AAB99126 ; MJ_1124 .
    GeneIDi 1452020.
    KEGGi mja:MJ_1124.

    Phylogenomic databases

    eggNOGi COG1372.
    KOi K03726.
    OMAi CLNANTE.

    Family and domain databases

    Gene3Di 2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.40.50.300. 5 hits.
    HAMAPi MF_00442. Helicase_Hel308.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR028992. Hedgehog/Intein_dom.
    IPR014001. Helicase_ATP-bd.
    IPR022965. Helicase_ski2.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00487. DEXDc. 1 hit.
    SM00278. HhH1. 2 hits.
    SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 3 hits.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

    Entry informationi

    Entry nameiHELS_METJA
    AccessioniPrimary (citable) accession number: Q58524
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3