Reviewed, UniProtKB/Swiss-Prot Q58524 (HELS_METJA)

Names and origin

Protein names

Recommended name:
    Putative ski2-type helicase
    EC=3.6.1.-
Cleaved into the following chain:
    1- Recommended name:
            Endonuclease PI-MjaHel
              EC=3.1.-.-
        Alternative name(s):
            Mja Hel intein
            Mja Pep3 intein

Gene names

Ordered Locus Names:

MJ1124

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	1195 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the helicase family. SKI2 subfamily. Contains 1 DOD-type homing endonuclease domain. Contains 1 helicase ATP-binding domain.

Ontologies

Keywords
Biological process	Intron homing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Endonuclease Helicase Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: HAMAP endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Putative ski2-type helicase, 1st part Potential		PRO_0000013299
	Chain	338 – 838	501	Endonuclease PI-MjaHel Potential		PRO_0000013300
	Chain	839 – 1195	357	Putative ski2-type helicase, 2nd part Potential		PRO_0000013301
Regions
	Domain	26 – 196	171	Helicase ATP-binding
	Domain	451 – 584	134	DOD-type homing endonuclease
	Nucleotide binding	39 – 46	8	ATP By similarity
	Motif	143 – 146	4	DEAH box HAMAP MF_00442
	Compositional bias	137 – 142	6	Poly-Val HAMAP MF_00442

Sequences

	Sequence		Length	Mass (Da)
	Q58524-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 87C9CF1A87EFB52D	FASTA	1,195	138,674
	10 20 30 40 50 60 MDKILEILKD FGIVELRPPQ KKALERGLLD KNKNFLISIP TASGKTLIGE MALINHLLDG 70 80 90 100 110 120 NKNPTNKKGI FIVPLKALAS EKYEEFKSKY ERYGLRIALS IGDYDEDEDL SKYHLIITTA 130 140 150 160 170 180 EKLDSLWRHK IDWINDVSVV VVDEIHLIND ETRGGTLEIL LTKLKEFNVQ IIGLSATIGN 190 200 210 220 230 240 PDELAEWLNA ELIVDDWRPV ELKKGIYKNE AIEFINGEIR EIKAVDNNDI YNLVVDCVKE 250 260 270 280 290 300 GGCCLVFCNT KRNAVNEAKK LNLKKFLTEE EKIRLKEIAE EILSILEPPT EMCKTLAECI 310 320 330 340 350 360 LNGSAFHHAG LTYQHRKIVE DAFRKRLIKV ICCTPTLCLN ANTEILQESG FRKITELNKD 370 380 390 400 410 420 EKVFALCGKE IKPVDGWKVH KTPQHEYNIV VKTVNGLEIT TTPNHIFLVK ENGSLKEKEA 430 440 450 460 470 480 KDLKVGDYVA TVDRIRVKEK DIDLSNGDLY FIGYFIGDGY TGVIEKNTLK ATPDLAFNPK 490 500 510 520 530 540 YPPNFDDSEL HKKYFLKCRI SKGVAHYIYS KKLRKIFNKL NMLTKDNKNI DAFCNLPLDK 550 560 570 580 590 600 LAYLIAGLFD SDGYIYLNRK NIEFYSISEK LVEQLQFVLL RFGIHSSIRK KKTKTMVSPT 610 620 630 640 650 660 NGKEYKCKDI YVLTIRDFMS IKRFYENIPL RHEEKRRKLE EIIKNKEIGQ IPSEFVALRF 670 680 690 700 710 720 TPIAKIWCDC GFSVDLTMFK PRTKRQRELN KKRVKLLFEL LDGKKLITNY KEYYSKRKNP 730 740 750 760 770 780 YFDFIVREKI NGNNYYSLNE KGRVLMSLLN KHIKDKENLE EMYNFLVNLE KCPICGKPIH 790 800 810 820 830 840 KEMRYSWKKE CYDGDIYWDR IKEIKKIKVN DKYAYDIELP DDGSNSHYIV ANGFIVHNSA 850 860 870 880 890 900 GLNLPCRRAI VKDLTRFTNK GMRYIPIMEI QQCIGRAGRP GLDPYGEGII VAKNDRDYLR 910 920 930 940 950 960 AYQALTQKPE PIYSKLSNQA VLRTQLLGLI ATGEIRDEYD LEWFIRNTFY AHQYGNLREV 970 980 990 1000 1010 1020 AKNINEVIRF LEENEFIIDF MPTELGKRVS ELYIDPLSAK FIIDGLEEME NEEEIYYLYL 1030 1040 1050 1060 1070 1080 ISKTLEMMPN LRVYNSEELN LIDEMDSLGI KSFEIEDLEA FKTAKMLYDW INEVPEDEIL 1090 1100 1110 1120 1130 1140 KRYKIEPGIL RYKVENAVWI MHALKEIAKL IGKSSDIPEK LEIRLEYGAK EDIIELLSIK 1150 1160 1170 1180 1190 YIGRVRARKL YNAGIRSIED IINNPSKVAS IIGEKIAKKI LDELGVKFGQ QKLSF

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB99126.1.
PIR	C64440.
RefSeq	NP_248116.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1452020.
GenomeReviews	Gene locus MJ1124 in contig L77117_GR.
KEGG	mja:MJ1124.
NMPDR	fig|243232.1.peg.1153.
TIGR	MJ1124.
Phylogenomic databases
HOGENOM	Q58524.
OMA	IVHNSAG
Family and domain databases
HAMAP	MF_00442. [Tree]
InterPro	IPR014001. DEAD-like_N. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR014021. Helicase_SF1/SF2_ATP-bd. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00487. DEXDc. 1 hit. SM00278. HhH1. 2 hits. SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HELS_METJA

Accession

Primary (citable) accession number: Q58524

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)