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Q58519 (DAPF_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diaminopimelate epimerase

Short name=DAP epimerase
EC=5.1.1.7
Gene names
Name:dapF
Ordered Locus Names:MJ1119
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus

Protein attributes

Sequence length295 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine By similarity. HAMAP-Rule MF_00197

Catalytic activity

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP-Rule MF_00197

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1. HAMAP-Rule MF_00197

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00197.

Sequence similarities

Belongs to the diaminopimelate epimerase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   Molecular functionIsomerase
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlysine biosynthetic process via diaminopimelate

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondiaminopimelate epimerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 295295Diaminopimelate epimerase HAMAP-Rule MF_00197
PRO_0000149886

Regions

Region8 – 92Substrate binding By similarity
Region76 – 783Substrate binding By similarity
Region228 – 2292Substrate binding By similarity
Region238 – 2392Substrate binding By similarity

Sites

Active site761Proton donor/acceptor By similarity
Active site2371Proton donor/acceptor By similarity
Binding site111Substrate By similarity
Binding site471Substrate By similarity
Binding site671Substrate By similarity
Binding site1711Substrate By similarity
Binding site2101Substrate By similarity
Site1731Important for catalytic activity By similarity
Site2281Important for catalytic activity By similarity

Amino acid modifications

Disulfide bond76 ↔ 237 HAMAP-Rule MF_00197

Sequences

Sequence LengthMass (Da)Tools
Q58519 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 8CB87BF46806982A

FASTA29533,442
        10         20         30         40         50         60 
MEFTKMHALG NDYIVINEFD GEKVKEEEKA EFSRKICRRG FSVGADGVIF IQKPTSDEYD 

        70         80         90        100        110        120 
VRFRIFNSDG SEAEMCGNGI RCFSKYVYER IMKKNPLKVE TKGGLRVSEM EIEGDEVKKI 

       130        140        150        160        170        180 
KVYMGVPKFK LKDIPMVVDG YKEDDEFLNG ELKLKNPYLP KVKLSVVNVG NPHAVIFVED 

       190        200        210        220        230        240 
NNIDLDFVRE HLDVIGKEIE HHEAFPERIN VHFVKVLNPN EIRIVTWERG AGYTTACGTG 

       250        260        270        280        290 
TTASVIMAHK LGKTNNRVLA HLDGGDLEIE IKDDGVYMIG DAVMVYDAKL INIGW 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L77117 Genomic DNA. Translation: AAB99120.1.
PIRF64439.
RefSeqNP_248111.1. NC_000909.1.

3D structure databases

ProteinModelPortalQ58519.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243232.MJ1119.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB99120; AAB99120; MJ_1119.
GeneID1452015.
KEGGmja:MJ_1119.

Phylogenomic databases

eggNOGCOG0253.
KOK01778.
OMACFARFVL.
ProtClustDBPRK00450.

Enzyme and pathway databases

UniPathwayUPA00034; UER00025.

Family and domain databases

HAMAPMF_00197. DAP_epimerase.
InterProIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERPTHR31689:SF0. PTHR31689:SF0. 1 hit.
PfamPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsTIGR00652. DapF. 1 hit.
PROSITEPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPF_METJA
AccessionPrimary (citable) accession number: Q58519
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names