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Protein

Putative asparagine synthetase [glutamine-hydrolyzing] 1

Gene

MJ1116

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Putative asparagine synthetase [glutamine-hydrolyzing] 1 (MJ1116), Putative asparagine synthetase [glutamine-hydrolyzing] 2 (MJ1056)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei116GlutamineBy similarity1
Binding sitei289ATP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi363 – 364ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Asparagine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMJAN243232:G1GKE-1211-MONOMER
UniPathwayiUPA00134; UER00195

Protein family/group databases

MEROPSiC44.001

Names & Taxonomyi

Protein namesi
Recommended name:
Putative asparagine synthetase [glutamine-hydrolyzing] 1 (EC:6.3.5.4)
Gene namesi
Ordered Locus Names:MJ1116
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000569372 – 541Putative asparagine synthetase [glutamine-hydrolyzing] 1Add BLAST540

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ_1116

Structurei

3D structure databases

ProteinModelPortaliQ58516
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 213Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST212

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 72Glutamine bindingBy similarity5
Regioni92 – 94Glutamine bindingBy similarity3

Sequence similaritiesi

Belongs to the asparagine synthetase family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00071 Archaea
COG0367 LUCA
InParanoidiQ58516
KOiK01953
OMAiYFHAAPD
OrthoDBiPOG093Z05K2
PhylomeDBiQ58516

Family and domain databases

CDDicd01991 Asn_Synthase_B_C, 1 hit
cd00712 AsnB, 1 hit
Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR006426 Asn_synth_AEB
IPR001962 Asn_synthase
IPR033738 AsnB_N
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
PfamiView protein in Pfam
PF00733 Asn_synthase, 1 hit
PIRSFiPIRSF001589 Asn_synthetase_glu-h, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01536 asn_synth_AEB, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58516-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCSISGIIVK DNQISAKYSI DMMKILKHRG RDNSGLLLDD EVIYFNDFED
60 70 80 90 100
VEDLEEEMIG NLSLAHNRLA IVGRYGVQPI PNEDETIWLV CNGEIYNYIE
110 120 130 140 150
LREYLKQNHE FRTDSDNEVI IHLYEEEKLE ELDGDYAFAI YDKSKNVVRL
160 170 180 190 200
ARDMFGVKPL FYVDRDKYFA FASERKALWH LLINIDGCER DLDELNSKIK
210 220 230 240 250
TLKPNSQLIY YLDDNRFEII EGFKKLELNY MKERSYEEAK EYLDRALKNS
260 270 280 290 300
VLKRVRGLDK VGIICSGGVD SSLIAKLASL YCEVILYAVG TENSEDLIYA
310 320 330 340 350
ERLAKDLNLK LRKKIISEEE YEEYVFKVAK AIDEVDLMKI GVGIPIYVAS
360 370 380 390 400
EMANEDGLKV VLSGQGADEL FGGYARHERI YRERGEEELK KELLKDVYNL
410 420 430 440 450
YKVNLERDDH CTMANGVELR VPFLDEEVVE IALSIPIEYK MSELSNRPYA
460 470 480 490 500
ESNISLKSEP INGLKNTNLN IKCVRSVRKK ILRDVASQYL PDYIAYRPKK
510 520 530 540
AAQYGSGGEK MIYKVAKKYG FSKKRINEFL DMLKRKIVSE F
Length:541
Mass (Da):62,655
Last modified:November 1, 1997 - v1
Checksum:i9B3FA64E39A957BB
GO

Sequence cautioni

The sequence AAB99117 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA Translation: AAB99117.1 Different initiation.
PIRiC64439
RefSeqiWP_064496729.1, NC_000909.1

Genome annotation databases

EnsemblBacteriaiAAB99117; AAB99117; MJ_1116
GeneIDi1452012
KEGGimja:MJ_1116

Similar proteinsi

Entry informationi

Entry nameiASNH1_METJA
AccessioniPrimary (citable) accession number: Q58516
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health