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Q58516

- ASNH1_METJA

UniProt

Q58516 - ASNH1_METJA

Protein

Putative asparagine synthetase [glutamine-hydrolyzing] 1

Gene

MJ1116

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei116 – 1161GlutamineBy similarity
    Binding sitei289 – 2891ATP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi363 – 3642ATPBy similarity

    GO - Molecular functioni

    1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Asparagine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00134; UER00195.

    Protein family/group databases

    MEROPSiC44.976.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative asparagine synthetase [glutamine-hydrolyzing] 1 (EC:6.3.5.4)
    Gene namesi
    Ordered Locus Names:MJ1116
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 541540Putative asparagine synthetase [glutamine-hydrolyzing] 1PRO_0000056937Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi243232.MJ1116.

    Structurei

    3D structure databases

    ProteinModelPortaliQ58516.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 213212Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni68 – 725Glutamine bindingBy similarity
    Regioni92 – 943Glutamine bindingBy similarity

    Sequence similaritiesi

    Belongs to the asparagine synthetase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0367.
    KOiK01953.
    OMAiIYVASEM.
    PhylomeDBiQ58516.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00733. Asn_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q58516-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCSISGIIVK DNQISAKYSI DMMKILKHRG RDNSGLLLDD EVIYFNDFED    50
    VEDLEEEMIG NLSLAHNRLA IVGRYGVQPI PNEDETIWLV CNGEIYNYIE 100
    LREYLKQNHE FRTDSDNEVI IHLYEEEKLE ELDGDYAFAI YDKSKNVVRL 150
    ARDMFGVKPL FYVDRDKYFA FASERKALWH LLINIDGCER DLDELNSKIK 200
    TLKPNSQLIY YLDDNRFEII EGFKKLELNY MKERSYEEAK EYLDRALKNS 250
    VLKRVRGLDK VGIICSGGVD SSLIAKLASL YCEVILYAVG TENSEDLIYA 300
    ERLAKDLNLK LRKKIISEEE YEEYVFKVAK AIDEVDLMKI GVGIPIYVAS 350
    EMANEDGLKV VLSGQGADEL FGGYARHERI YRERGEEELK KELLKDVYNL 400
    YKVNLERDDH CTMANGVELR VPFLDEEVVE IALSIPIEYK MSELSNRPYA 450
    ESNISLKSEP INGLKNTNLN IKCVRSVRKK ILRDVASQYL PDYIAYRPKK 500
    AAQYGSGGEK MIYKVAKKYG FSKKRINEFL DMLKRKIVSE F 541
    Length:541
    Mass (Da):62,655
    Last modified:November 1, 1997 - v1
    Checksum:i9B3FA64E39A957BB
    GO

    Sequence cautioni

    The sequence AAB99117.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99117.1. Different initiation.
    PIRiC64439.
    RefSeqiNP_248108.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB99117; AAB99117; MJ_1116.
    GeneIDi1452012.
    KEGGimja:MJ_1116.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99117.1 . Different initiation.
    PIRi C64439.
    RefSeqi NP_248108.1. NC_000909.1.

    3D structure databases

    ProteinModelPortali Q58516.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ1116.

    Protein family/group databases

    MEROPSi C44.976.

    Protocols and materials databases

    DNASUi 1452012.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB99117 ; AAB99117 ; MJ_1116 .
    GeneIDi 1452012.
    KEGGi mja:MJ_1116.

    Phylogenomic databases

    eggNOGi COG0367.
    KOi K01953.
    OMAi IYVASEM.
    PhylomeDBi Q58516.

    Enzyme and pathway databases

    UniPathwayi UPA00134 ; UER00195 .

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00733. Asn_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

    Entry informationi

    Entry nameiASNH1_METJA
    AccessioniPrimary (citable) accession number: Q58516
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3