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Protein

Putative asparagine synthetase [glutamine-hydrolyzing] 1

Gene

MJ1116

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei116 – 1161GlutamineBy similarity
Binding sitei289 – 2891ATP; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi363 – 3642ATPBy similarity

GO - Molecular functioni

  1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative asparagine synthetase [glutamine-hydrolyzing] 1 (EC:6.3.5.4)
Gene namesi
Ordered Locus Names:MJ1116
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 541540Putative asparagine synthetase [glutamine-hydrolyzing] 1PRO_0000056937Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ1116.

Structurei

3D structure databases

ProteinModelPortaliQ58516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 213212Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni68 – 725Glutamine bindingBy similarity
Regioni92 – 943Glutamine bindingBy similarity

Sequence similaritiesi

Belongs to the asparagine synthetase family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0367.
InParanoidiQ58516.
KOiK01953.
OMAiEYKIEEP.
PhylomeDBiQ58516.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58516-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCSISGIIVK DNQISAKYSI DMMKILKHRG RDNSGLLLDD EVIYFNDFED
60 70 80 90 100
VEDLEEEMIG NLSLAHNRLA IVGRYGVQPI PNEDETIWLV CNGEIYNYIE
110 120 130 140 150
LREYLKQNHE FRTDSDNEVI IHLYEEEKLE ELDGDYAFAI YDKSKNVVRL
160 170 180 190 200
ARDMFGVKPL FYVDRDKYFA FASERKALWH LLINIDGCER DLDELNSKIK
210 220 230 240 250
TLKPNSQLIY YLDDNRFEII EGFKKLELNY MKERSYEEAK EYLDRALKNS
260 270 280 290 300
VLKRVRGLDK VGIICSGGVD SSLIAKLASL YCEVILYAVG TENSEDLIYA
310 320 330 340 350
ERLAKDLNLK LRKKIISEEE YEEYVFKVAK AIDEVDLMKI GVGIPIYVAS
360 370 380 390 400
EMANEDGLKV VLSGQGADEL FGGYARHERI YRERGEEELK KELLKDVYNL
410 420 430 440 450
YKVNLERDDH CTMANGVELR VPFLDEEVVE IALSIPIEYK MSELSNRPYA
460 470 480 490 500
ESNISLKSEP INGLKNTNLN IKCVRSVRKK ILRDVASQYL PDYIAYRPKK
510 520 530 540
AAQYGSGGEK MIYKVAKKYG FSKKRINEFL DMLKRKIVSE F
Length:541
Mass (Da):62,655
Last modified:November 1, 1997 - v1
Checksum:i9B3FA64E39A957BB
GO

Sequence cautioni

The sequence AAB99117.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99117.1. Different initiation.
PIRiC64439.
RefSeqiNP_248108.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99117; AAB99117; MJ_1116.
GeneIDi1452012.
KEGGimja:MJ_1116.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99117.1. Different initiation.
PIRiC64439.
RefSeqiNP_248108.1. NC_000909.1.

3D structure databases

ProteinModelPortaliQ58516.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ1116.

Protocols and materials databases

DNASUi1452012.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99117; AAB99117; MJ_1116.
GeneIDi1452012.
KEGGimja:MJ_1116.

Phylogenomic databases

eggNOGiCOG0367.
InParanoidiQ58516.
KOiK01953.
OMAiEYKIEEP.
PhylomeDBiQ58516.

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiASNH1_METJA
AccessioniPrimary (citable) accession number: Q58516
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 1, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.