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Protein

Homoserine kinase

Gene

thrB

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.By similarity

Catalytic activityi

ATP + L-homoserine = ADP + O-phospho-L-homoserine.

Pathway: L-threonine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable aspartokinase (MJ0571)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Homoserine dehydrogenase (hom)
  4. Homoserine kinase (thrB)
  5. Threonine synthase (thrC)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 9611ATPSequence AnalysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Threonine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14633.
BRENDAi2.7.1.39. 3260.
SABIO-RKQ58504.
UniPathwayiUPA00050; UER00064.

Names & Taxonomyi

Protein namesi
Recommended name:
Homoserine kinase (EC:2.7.1.39)
Short name:
HK
Short name:
HSK
Gene namesi
Name:thrB
Ordered Locus Names:MJ1104
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296Homoserine kinasePRO_0000156641Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ_1104.

Structurei

Secondary structure

1
296
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 1211Combined sources
Helixi15 – 173Combined sources
Turni18 – 203Combined sources
Beta strandi21 – 4727Combined sources
Turni55 – 573Combined sources
Helixi59 – 7012Combined sources
Beta strandi76 – 827Combined sources
Beta strandi89 – 913Combined sources
Helixi93 – 10816Combined sources
Helixi115 – 13016Combined sources
Helixi138 – 1436Combined sources
Beta strandi145 – 1517Combined sources
Turni152 – 1554Combined sources
Beta strandi156 – 1605Combined sources
Beta strandi168 – 1714Combined sources
Helixi179 – 1846Combined sources
Beta strandi188 – 1914Combined sources
Helixi192 – 21019Combined sources
Helixi214 – 2218Combined sources
Helixi228 – 2325Combined sources
Helixi238 – 2458Combined sources
Helixi246 – 2483Combined sources
Beta strandi249 – 2546Combined sources
Beta strandi261 – 2655Combined sources
Helixi267 – 2693Combined sources
Helixi270 – 28011Combined sources
Beta strandi284 – 2874Combined sources
Beta strandi293 – 2953Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FWKX-ray2.10A/B/C/D1-296[»]
1FWLX-ray2.25A/B/C/D1-296[»]
1H72X-ray1.80C1-296[»]
1H73X-ray2.00A1-296[»]
1H74X-ray1.90A/B/C/D1-296[»]
ProteinModelPortaliQ58504.
SMRiQ58504. Positions 1-296.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ58504.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0083.
InParanoidiQ58504.
KOiK00872.
OMAiAPHADNV.
PhylomeDBiQ58504.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00384. Homoser_kinase.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR000870. Homoserine_kinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000676. Homoser_kin. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00191. thrB. 1 hit.
PROSITEiPS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q58504-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVRVKAPCT SANLGVGFDV FGLCLKEPYD VIEVEAIDDK EIIIEVDDKN
60 70 80 90 100
IPTDPDKNVA GIVAKKMIDD FNIGKGVKIT IKKGVKAGSG LGSSAASSAG
110 120 130 140 150
TAYAINELFK LNLDKLKLVD YASYGELASS GAKHADNVAP AIFGGFTMVT
160 170 180 190 200
NYEPLEVLHI PIDFKLDILI AIPNISINTK EAREILPKAV GLKDLVNNVG
210 220 230 240 250
KACGMVYALY NKDKSLFGRY MMSDKVIEPV RGKLIPNYFK IKEEVKDKVY
260 270 280 290
GITISGSGPS IIAFPKEEFI DEVENILRDY YENTIRTEVG KGVEVV
Length:296
Mass (Da):32,259
Last modified:June 1, 2001 - v2
Checksum:i49919E68F3834C78
GO

Sequence cautioni

The sequence AAB99107.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99107.1. Different initiation.
PIRiG64437.
RefSeqiNP_248097.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99107; AAB99107; MJ_1104.
GeneIDi1452001.
KEGGimja:MJ_1104.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99107.1. Different initiation.
PIRiG64437.
RefSeqiNP_248097.1. NC_000909.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FWKX-ray2.10A/B/C/D1-296[»]
1FWLX-ray2.25A/B/C/D1-296[»]
1H72X-ray1.80C1-296[»]
1H73X-ray2.00A1-296[»]
1H74X-ray1.90A/B/C/D1-296[»]
ProteinModelPortaliQ58504.
SMRiQ58504. Positions 1-296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_1104.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99107; AAB99107; MJ_1104.
GeneIDi1452001.
KEGGimja:MJ_1104.

Phylogenomic databases

eggNOGiCOG0083.
InParanoidiQ58504.
KOiK00872.
OMAiAPHADNV.
PhylomeDBiQ58504.

Enzyme and pathway databases

UniPathwayiUPA00050; UER00064.
BioCyciMetaCyc:MONOMER-14633.
BRENDAi2.7.1.39. 3260.
SABIO-RKQ58504.

Miscellaneous databases

EvolutionaryTraceiQ58504.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00384. Homoser_kinase.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR000870. Homoserine_kinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000676. Homoser_kin. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00191. thrB. 1 hit.
PROSITEiPS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  2. "Structure and mechanism of homoserine kinase: prototype for the GHMP kinase superfamily."
    Zhou T., Daugherty M., Grishin N.V., Osterman A.L., Zhang H.
    Structure 8:1247-1257(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Entry informationi

Entry nameiKHSE_METJA
AccessioniPrimary (citable) accession number: Q58504
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 1, 2001
Last modified: June 24, 2015
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.