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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Pathwayi

GO - Molecular functioni

  1. dUTP diphosphatase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. dUMP biosynthetic process Source: UniProtKB-UniPathway
  2. dUTP metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Enzyme and pathway databases

SABIO-RKQ58502.
UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
MjDUT
dUTP pyrophosphatase
Gene namesi
Name:dut
Ordered Locus Names:MJ1102
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 156155Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_0000153638Add
BLAST

Interactioni

Subunit structurei

Homotrimer.Curated

Protein-protein interaction databases

STRINGi243232.MJ1102.

Structurei

3D structure databases

ProteinModelPortaliQ58502.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0717.
InParanoidiQ58502.
KOiK01520.
OMAiENVAGFA.
PhylomeDBiQ58502.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
HAMAPiMF_00635. dUTPase_arch.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR023537. dUTPase_archaeal.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58502-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIGANTSKN FFDNLEEEQI QQCGIDLRVW KIFKIEGEGV IDFSNEKRKL
60 70 80 90 100
PNYIEIFNSE KDEHIKLDRG VYIVKVADYI KIPENVAGFA YPRSSLLRMG
110 120 130 140 150
ATLYSAVHDP GYEGRPEYLM QVFNPITIYK YARIAQIVFV ECRDVKGVYE

GIYKGR
Length:156
Mass (Da):18,070
Last modified:January 23, 2007 - v4
Checksum:iAF6E28A4CF91FF3D
GO

Sequence cautioni

The sequence AAB99105.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99105.1. Different initiation.
PIRiE64437.
RefSeqiNP_248095.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99105; AAB99105; MJ_1102.
GeneIDi1451999.
KEGGimja:MJ_1102.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99105.1. Different initiation.
PIRiE64437.
RefSeqiNP_248095.1. NC_000909.1.

3D structure databases

ProteinModelPortaliQ58502.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ1102.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99105; AAB99105; MJ_1102.
GeneIDi1451999.
KEGGimja:MJ_1102.

Phylogenomic databases

eggNOGiCOG0717.
InParanoidiQ58502.
KOiK01520.
OMAiENVAGFA.
PhylomeDBiQ58502.

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.
SABIO-RKQ58502.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
HAMAPiMF_00635. dUTPase_arch.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR023537. dUTPase_archaeal.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  2. "A bifunctional dCTP deaminase-dUTP nucleotidohydrolase from the hyperthermophilic archaeon Methanocaldococcus jannaschii."
    Bjoernberg O., Neuhard J., Nyman P.O.
    J. Biol. Chem. 278:20667-20672(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-10, CHARACTERIZATION.
  3. "The Methanococcus jannaschii dCTP deaminase is a bifunctional deaminase and diphosphatase."
    Li H., Xu H., Graham D.E., White R.H.
    J. Biol. Chem. 278:11100-11106(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiDUT_METJA
AccessioniPrimary (citable) accession number: Q58502
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 87 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.