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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.UniRule annotation2 Publications

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotation2 Publications

Kineticsi

kcat is 4.6 sec(-1).1 Publication
  1. KM=0.4 µM for dUTP1 Publication
  2. KM=103 µM for dUTP1 Publication

    Pathwayi: dUMP biosynthesis

    This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).UniRule annotation2 Publications
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
    This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    Biological processNucleotide metabolism

    Enzyme and pathway databases

    SABIO-RKiQ58502.
    UniPathwayiUPA00610; UER00666.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyuridine 5'-triphosphate nucleotidohydrolaseCurated (EC:3.6.1.23UniRule annotation2 Publications)
    Short name:
    dUTPaseUniRule annotation
    Alternative name(s):
    MjDUT
    dUTP pyrophosphataseUniRule annotation
    Gene namesi
    Name:dutUniRule annotation
    Ordered Locus Names:MJ1102
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    Proteomesi
    • UP000000805 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00001536382 – 156Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST155

    Interactioni

    Subunit structurei

    Homotrimer.1 Publication

    Protein-protein interaction databases

    STRINGi243232.MJ_1102.

    Structurei

    3D structure databases

    ProteinModelPortaliQ58502.
    SMRiQ58502.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the dCTP deaminase family. Archaeal dUTPase subfamily.UniRule annotationCurated

    Phylogenomic databases

    eggNOGiarCOG04048. Archaea.
    COG0717. LUCA.
    InParanoidiQ58502.
    KOiK01520.
    OMAiWDAGYEG.
    OrthoDBiPOG093Z0E8X.
    PhylomeDBiQ58502.

    Family and domain databases

    CDDicd07557. trimeric_dUTPase. 1 hit.
    HAMAPiMF_00635. dUTPase_arch. 1 hit.
    InterProiView protein in InterPro
    IPR029054. dUTPase-like.
    IPR023537. dUTPase_archaeal.
    IPR033704. dUTPase_trimeric.
    PfamiView protein in Pfam
    PF00692. dUTPase. 1 hit.
    SUPFAMiSSF51283. SSF51283. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q58502-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIIGANTSKN FFDNLEEEQI QQCGIDLRVW KIFKIEGEGV IDFSNEKRKL
    60 70 80 90 100
    PNYIEIFNSE KDEHIKLDRG VYIVKVADYI KIPENVAGFA YPRSSLLRMG
    110 120 130 140 150
    ATLYSAVHDP GYEGRPEYLM QVFNPITIYK YARIAQIVFV ECRDVKGVYE

    GIYKGR
    Length:156
    Mass (Da):18,070
    Last modified:January 23, 2007 - v4
    Checksum:iAF6E28A4CF91FF3D
    GO

    Sequence cautioni

    The sequence AAB99105 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L77117 Genomic DNA. Translation: AAB99105.1. Different initiation.
    PIRiE64437.
    RefSeqiWP_064496724.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB99105; AAB99105; MJ_1102.
    GeneIDi1451999.
    KEGGimja:MJ_1102.

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiDUT_METJA
    AccessioniPrimary (citable) accession number: Q58502
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 23, 2003
    Last sequence update: January 23, 2007
    Last modified: June 7, 2017
    This is version 99 of the entry and version 4 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families