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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.UniRule annotation2 Publications

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotation2 Publications

Kineticsi

kcat is 4.6 sec(-1).1 Publication
  1. KM=0.4 µM for dUTP1 Publication
  2. KM=103 µM for dUTP1 Publication

    Pathwayi: dUMP biosynthesis

    This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).UniRule annotation2 Publications
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
    This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    Biological processNucleotide metabolism

    Enzyme and pathway databases

    BioCyciMJAN243232:G1GKE-1196-MONOMER
    SABIO-RKiQ58502
    UniPathwayiUPA00610; UER00666

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyuridine 5'-triphosphate nucleotidohydrolaseCurated (EC:3.6.1.23UniRule annotation2 Publications)
    Short name:
    dUTPaseUniRule annotation
    Alternative name(s):
    MjDUT
    dUTP pyrophosphataseUniRule annotation
    Gene namesi
    Name:dutUniRule annotation
    Ordered Locus Names:MJ1102
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    Proteomesi
    • UP000000805 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00001536382 – 156Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST155

    Proteomic databases

    PRIDEiQ58502

    Interactioni

    Subunit structurei

    Homotrimer.1 Publication

    Protein-protein interaction databases

    STRINGi243232.MJ_1102

    Structurei

    3D structure databases

    ProteinModelPortaliQ58502
    SMRiQ58502
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the dCTP deaminase family. Archaeal dUTPase subfamily.UniRule annotationCurated

    Phylogenomic databases

    eggNOGiarCOG04048 Archaea
    COG0717 LUCA
    InParanoidiQ58502
    KOiK01520
    OMAiVHDPGYE
    OrthoDBiPOG093Z0E8X
    PhylomeDBiQ58502

    Family and domain databases

    CDDicd07557 trimeric_dUTPase, 1 hit
    Gene3Di2.70.40.10, 1 hit
    HAMAPiMF_00635 dUTPase_arch, 1 hit
    InterProiView protein in InterPro
    IPR029054 dUTPase-like
    IPR036157 dUTPase-like_sf
    IPR023537 dUTPase_archaeal
    IPR033704 dUTPase_trimeric
    PfamiView protein in Pfam
    PF00692 dUTPase, 1 hit
    SUPFAMiSSF51283 SSF51283, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q58502-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIIGANTSKN FFDNLEEEQI QQCGIDLRVW KIFKIEGEGV IDFSNEKRKL
    60 70 80 90 100
    PNYIEIFNSE KDEHIKLDRG VYIVKVADYI KIPENVAGFA YPRSSLLRMG
    110 120 130 140 150
    ATLYSAVHDP GYEGRPEYLM QVFNPITIYK YARIAQIVFV ECRDVKGVYE

    GIYKGR
    Length:156
    Mass (Da):18,070
    Last modified:January 23, 2007 - v4
    Checksum:iAF6E28A4CF91FF3D
    GO

    Sequence cautioni

    The sequence AAB99105 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L77117 Genomic DNA Translation: AAB99105.1 Different initiation.
    PIRiE64437
    RefSeqiWP_064496724.1, NC_000909.1

    Genome annotation databases

    EnsemblBacteriaiAAB99105; AAB99105; MJ_1102
    GeneIDi1451999
    KEGGimja:MJ_1102

    Similar proteinsi

    Entry informationi

    Entry nameiDUT_METJA
    AccessioniPrimary (citable) accession number: Q58502
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 23, 2003
    Last sequence update: January 23, 2007
    Last modified: May 23, 2018
    This is version 104 of the entry and version 4 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

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