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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. no protein annotated in this organism
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei66ShikimateUniRule annotation1
Active sitei70Proton acceptorUniRule annotation1
Binding sitei91ShikimateUniRule annotation1
Binding sitei106ShikimateUniRule annotation1
Binding sitei196NADP; via carbonyl oxygenUniRule annotation1 Publication1
Binding sitei200NADP1 Publication1
Binding sitei224NADP; via carbonyl oxygen1 Publication1
Binding sitei226ShikimateUniRule annotation1
Binding sitei247NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi130 – 134NADPUniRule annotation1 Publication5
Nucleotide bindingi152 – 157NADPUniRule annotation1 Publication6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18802.
BRENDAi1.1.1.25. 3260.
UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:MJ1084
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001360601 – 282Shikimate dehydrogenase (NADP(+))Add BLAST282

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi243232.MJ_1084.

Structurei

Secondary structure

1282
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 15Combined sources9
Helixi21 – 31Combined sources11
Beta strandi36 – 42Combined sources7
Helixi45 – 50Combined sources6
Helixi51 – 58Combined sources8
Beta strandi62 – 65Combined sources4
Helixi72 – 76Combined sources5
Beta strandi78 – 80Combined sources3
Helixi82 – 87Combined sources6
Beta strandi92 – 96Combined sources5
Beta strandi99 – 103Combined sources5
Helixi106 – 118Combined sources13
Beta strandi125 – 129Combined sources5
Helixi133 – 142Combined sources10
Beta strandi144 – 151Combined sources8
Helixi155 – 169Combined sources15
Helixi173 – 176Combined sources4
Beta strandi177 – 180Combined sources4
Beta strandi191 – 194Combined sources4
Beta strandi217 – 223Combined sources7
Beta strandi227 – 230Combined sources4
Helixi232 – 238Combined sources7
Turni239 – 241Combined sources3
Beta strandi243 – 245Combined sources3
Helixi248 – 263Combined sources16
Helixi269 – 280Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NVTX-ray2.35A/B1-282[»]
ProteinModelPortaliQ58484.
SMRiQ58484.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ58484.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 21Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01033. Archaea.
COG0169. LUCA.
InParanoidiQ58484.
KOiK00014.
OMAiSIYIDAT.
PhylomeDBiQ58484.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q58484-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINAKTKVIG LIGHPVEHSF SPIMHNAAFK DKGLNYVYVA FDVLPENLKY
60 70 80 90 100
VIDGAKALGI VGFNVTIPHK IEIMKYLDEI DKDAQLIGAV NTIKIEDGKA
110 120 130 140 150
IGYNTDGIGA RMALEEEIGR VKDKNIVIYG AGGAARAVAF ELAKDNNIII
160 170 180 190 200
ANRTVEKAEA LAKEIAEKLN KKFGEEVKFS GLDVDLDGVD IIINATPIGM
210 220 230 240 250
YPNIDVEPIV KAEKLREDMV VMDLIYNPLE TVLLKEAKKV NAKTINGLGM
260 270 280
LIYQGAVAFK IWTGVEPNIE VMKNAIIDKI TK
Length:282
Mass (Da):30,877
Last modified:November 1, 1996 - v1
Checksum:iE7398E5D642F7BD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99086.1.
PIRiC64435.
RefSeqiWP_010870596.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99086; AAB99086; MJ_1084.
GeneIDi1451980.
KEGGimja:MJ_1084.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99086.1.
PIRiC64435.
RefSeqiWP_010870596.1. NC_000909.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NVTX-ray2.35A/B1-282[»]
ProteinModelPortaliQ58484.
SMRiQ58484.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_1084.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99086; AAB99086; MJ_1084.
GeneIDi1451980.
KEGGimja:MJ_1084.

Phylogenomic databases

eggNOGiarCOG01033. Archaea.
COG0169. LUCA.
InParanoidiQ58484.
KOiK00014.
OMAiSIYIDAT.
PhylomeDBiQ58484.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.
BioCyciMetaCyc:MONOMER-18802.
BRENDAi1.1.1.25. 3260.

Miscellaneous databases

EvolutionaryTraceiQ58484.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROE_METJA
AccessioniPrimary (citable) accession number: Q58484
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.