Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathway: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. no protein annotated in this organism
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei66 – 661ShikimateUniRule annotation
Active sitei70 – 701Proton acceptorUniRule annotation
Binding sitei91 – 911ShikimateUniRule annotation
Binding sitei106 – 1061ShikimateUniRule annotation
Binding sitei196 – 1961NADP; via carbonyl oxygenUniRule annotation1 Publication
Binding sitei200 – 2001NADP1 Publication
Binding sitei224 – 2241NADP; via carbonyl oxygen1 Publication
Binding sitei226 – 2261ShikimateUniRule annotation
Binding sitei247 – 2471NADP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi130 – 1345NADPUniRule annotation1 Publication
Nucleotide bindingi152 – 1576NADPUniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.25. 3260.
UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:MJ1084
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282Shikimate dehydrogenase (NADP(+))PRO_0000136060Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi243232.MJ_1084.

Structurei

Secondary structure

1
282
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 159Combined sources
Helixi21 – 3111Combined sources
Beta strandi36 – 427Combined sources
Helixi45 – 506Combined sources
Helixi51 – 588Combined sources
Beta strandi62 – 654Combined sources
Helixi72 – 765Combined sources
Beta strandi78 – 803Combined sources
Helixi82 – 876Combined sources
Beta strandi92 – 965Combined sources
Beta strandi99 – 1035Combined sources
Helixi106 – 11813Combined sources
Beta strandi125 – 1295Combined sources
Helixi133 – 14210Combined sources
Beta strandi144 – 1518Combined sources
Helixi155 – 16915Combined sources
Helixi173 – 1764Combined sources
Beta strandi177 – 1804Combined sources
Beta strandi191 – 1944Combined sources
Beta strandi217 – 2237Combined sources
Beta strandi227 – 2304Combined sources
Helixi232 – 2387Combined sources
Turni239 – 2413Combined sources
Beta strandi243 – 2453Combined sources
Helixi248 – 26316Combined sources
Helixi269 – 28012Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NVTX-ray2.35A/B1-282[»]
ProteinModelPortaliQ58484.
SMRiQ58484. Positions 1-282.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ58484.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 213Shikimate bindingUniRule annotation

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0169.
InParanoidiQ58484.
KOiK00014.
OMAiSIYIDAT.
PhylomeDBiQ58484.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q58484-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINAKTKVIG LIGHPVEHSF SPIMHNAAFK DKGLNYVYVA FDVLPENLKY
60 70 80 90 100
VIDGAKALGI VGFNVTIPHK IEIMKYLDEI DKDAQLIGAV NTIKIEDGKA
110 120 130 140 150
IGYNTDGIGA RMALEEEIGR VKDKNIVIYG AGGAARAVAF ELAKDNNIII
160 170 180 190 200
ANRTVEKAEA LAKEIAEKLN KKFGEEVKFS GLDVDLDGVD IIINATPIGM
210 220 230 240 250
YPNIDVEPIV KAEKLREDMV VMDLIYNPLE TVLLKEAKKV NAKTINGLGM
260 270 280
LIYQGAVAFK IWTGVEPNIE VMKNAIIDKI TK
Length:282
Mass (Da):30,877
Last modified:November 1, 1996 - v1
Checksum:iE7398E5D642F7BD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99086.1.
PIRiC64435.
RefSeqiNP_248077.1. NC_000909.1.
WP_010870596.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99086; AAB99086; MJ_1084.
GeneIDi1451980.
KEGGimja:MJ_1084.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99086.1.
PIRiC64435.
RefSeqiNP_248077.1. NC_000909.1.
WP_010870596.1. NC_000909.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NVTX-ray2.35A/B1-282[»]
ProteinModelPortaliQ58484.
SMRiQ58484. Positions 1-282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_1084.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99086; AAB99086; MJ_1084.
GeneIDi1451980.
KEGGimja:MJ_1084.

Phylogenomic databases

eggNOGiCOG0169.
InParanoidiQ58484.
KOiK00014.
OMAiSIYIDAT.
PhylomeDBiQ58484.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.
BRENDAi1.1.1.25. 3260.

Miscellaneous databases

EvolutionaryTraceiQ58484.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  2. "Crystal structure of shikimate 5-dehydrogenase (SDH) bound to NADP: insights into function and evolution."
    Padyana A.K., Burley S.K.
    Structure 11:1005-1013(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEX WITH NADP, SUBUNIT.

Entry informationi

Entry nameiAROE_METJA
AccessioniPrimary (citable) accession number: Q58484
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.