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Q58484

- AROE_METJA

UniProt

Q58484 - AROE_METJA

Protein

Shikimate dehydrogenase

Gene

aroE

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Shikimate + NADP+ = 3-dehydroshikimate + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei70 – 701Proton acceptorSequence Analysis
    Binding sitei106 – 1061SubstrateBy similarity
    Binding sitei196 – 1961NADP; via carbonyl oxygen1 Publication
    Binding sitei200 – 2001NADP1 Publication
    Binding sitei224 – 2241NADP; via carbonyl oxygen1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi130 – 1345NADP1 Publication
    Nucleotide bindingi152 – 1576NADP1 Publication
    Nucleotide bindingi247 – 2515NADPBy similarity

    GO - Molecular functioni

    1. NADP binding Source: InterPro
    2. shikimate 3-dehydrogenase (NADP+) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. aromatic amino acid family biosynthetic process Source: UniProtKB-HAMAP
    2. chorismate biosynthetic process Source: UniProtKB-UniPathway
    3. shikimate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00053; UER00087.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Shikimate dehydrogenase (EC:1.1.1.25)
    Gene namesi
    Name:aroE
    Ordered Locus Names:MJ1084
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 282282Shikimate dehydrogenasePRO_0000136060Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi243232.MJ1084.

    Structurei

    Secondary structure

    1
    282
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 159
    Helixi21 – 3111
    Beta strandi36 – 427
    Helixi45 – 506
    Helixi51 – 588
    Beta strandi62 – 654
    Helixi72 – 765
    Beta strandi78 – 803
    Helixi82 – 876
    Beta strandi92 – 965
    Beta strandi99 – 1035
    Helixi106 – 11813
    Beta strandi125 – 1295
    Helixi133 – 14210
    Beta strandi144 – 1518
    Helixi155 – 16915
    Helixi173 – 1764
    Beta strandi177 – 1804
    Beta strandi191 – 1944
    Beta strandi217 – 2237
    Beta strandi227 – 2304
    Helixi232 – 2387
    Turni239 – 2413
    Beta strandi243 – 2453
    Helixi248 – 26316
    Helixi269 – 28012

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NVTX-ray2.35A/B1-282[»]
    ProteinModelPortaliQ58484.
    SMRiQ58484. Positions 1-282.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ58484.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the shikimate dehydrogenase family.Curated

    Phylogenomic databases

    eggNOGiCOG0169.
    KOiK00014.
    OMAiDYVYLPF.
    PhylomeDBiQ58484.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00222. Shikimate_DH_AroE.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR011342. Shikimate_DH.
    IPR013708. Shikimate_DH-bd_N.
    IPR022893. Shikimate_quinate_DH.
    IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
    [Graphical view]
    PfamiPF01488. Shikimate_DH. 1 hit.
    PF08501. Shikimate_dh_N. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00507. aroE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q58484-1 [UniParc]FASTAAdd to Basket

    « Hide

    MINAKTKVIG LIGHPVEHSF SPIMHNAAFK DKGLNYVYVA FDVLPENLKY    50
    VIDGAKALGI VGFNVTIPHK IEIMKYLDEI DKDAQLIGAV NTIKIEDGKA 100
    IGYNTDGIGA RMALEEEIGR VKDKNIVIYG AGGAARAVAF ELAKDNNIII 150
    ANRTVEKAEA LAKEIAEKLN KKFGEEVKFS GLDVDLDGVD IIINATPIGM 200
    YPNIDVEPIV KAEKLREDMV VMDLIYNPLE TVLLKEAKKV NAKTINGLGM 250
    LIYQGAVAFK IWTGVEPNIE VMKNAIIDKI TK 282
    Length:282
    Mass (Da):30,877
    Last modified:November 1, 1996 - v1
    Checksum:iE7398E5D642F7BD1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99086.1.
    PIRiC64435.
    RefSeqiNP_248077.1. NC_000909.1.
    WP_010870596.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB99086; AAB99086; MJ_1084.
    GeneIDi1451980.
    KEGGimja:MJ_1084.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99086.1 .
    PIRi C64435.
    RefSeqi NP_248077.1. NC_000909.1.
    WP_010870596.1. NC_000909.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1NVT X-ray 2.35 A/B 1-282 [» ]
    ProteinModelPortali Q58484.
    SMRi Q58484. Positions 1-282.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ1084.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB99086 ; AAB99086 ; MJ_1084 .
    GeneIDi 1451980.
    KEGGi mja:MJ_1084.

    Phylogenomic databases

    eggNOGi COG0169.
    KOi K00014.
    OMAi DYVYLPF.
    PhylomeDBi Q58484.

    Enzyme and pathway databases

    UniPathwayi UPA00053 ; UER00087 .

    Miscellaneous databases

    EvolutionaryTracei Q58484.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_00222. Shikimate_DH_AroE.
    InterProi IPR016040. NAD(P)-bd_dom.
    IPR011342. Shikimate_DH.
    IPR013708. Shikimate_DH-bd_N.
    IPR022893. Shikimate_quinate_DH.
    IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
    [Graphical view ]
    Pfami PF01488. Shikimate_DH. 1 hit.
    PF08501. Shikimate_dh_N. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00507. aroE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
    2. "Crystal structure of shikimate 5-dehydrogenase (SDH) bound to NADP: insights into function and evolution."
      Padyana A.K., Burley S.K.
      Structure 11:1005-1013(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEX WITH NADP, SUBUNIT.

    Entry informationi

    Entry nameiAROE_METJA
    AccessioniPrimary (citable) accession number: Q58484
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3