Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q58456 (ASNH2_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative asparagine synthetase [glutamine-hydrolyzing] 2

EC=6.3.5.4
Gene names
Ordered Locus Names:MJ1056
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus

Protein attributes

Sequence length515 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway

Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.

Sequence similarities

Belongs to the asparagine synthetase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence caution

The sequence AAB99058.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 515514Putative asparagine synthetase [glutamine-hydrolyzing] 2
PRO_0000056938

Regions

Domain2 – 229228Glutamine amidotransferase type-2
Nucleotide binding378 – 3792ATP By similarity
Region52 – 565Glutamine binding By similarity
Region92 – 943Glutamine binding By similarity

Sites

Active site21For GATase activity By similarity
Binding site1141Glutamine By similarity
Binding site3061ATP; via amide nitrogen and carbonyl oxygen By similarity
Site3801Important for beta-aspartyl-AMP intermediate formation By similarity

Sequences

Sequence LengthMass (Da)Tools
Q58456 [UniParc].

Last modified September 26, 2003. Version 3.
Checksum: EE9EFBD4E643EDEB

FASTA51560,473
        10         20         30         40         50         60 
MCGINGIIRF GKEVIKEEIN KMNKAIKHRG PDDEGIFIYN FKNYSIGLGH VRLAILDLSE 

        70         80         90        100        110        120 
KGHQPMGYNV DEDKIIYRDD ELDRADIIIV YNGEIYNYLE LKEKFNLETE TGTDTEVILK 

       130        140        150        160        170        180 
LYNKLGFDCV KEFNGMWAFC IFDKKKGLIF CSRDRLGVKP FYYYWDGNEF IFSSELKGIL 

       190        200        210        220        230        240 
AVKEINKKEN INKDAVELYF ALGFIPSPYS IYKNTFKLEA RQNLIFDLDK REIRKYYYWE 

       250        260        270        280        290        300 
LPDYKPIYDK KKLIEEGKKL LYDAVKIRMR SDVPVGAFLS GGLDSSTVVG VMREFTDLSK 

       310        320        330        340        350        360 
LHTFSIGFEG KYDETPYIKI VVDYFKTQHH HYYFKERDFE ELIDKYSWIY DEPFGDYSGF 

       370        380        390        400        410        420 
PTYKVSEMAR KFVTVVLSGD GGDEVFGGYM THLNGYRMDF IRKLPKFLRV VGSKLPVKKD 

       430        440        450        460        470        480 
LNGIANLYLL KEAFRLSLIN PEEFYAESIK EDAIRPEIYK KWTIEKLRYC LNKGDNKLGE 

       490        500        510 
ALRIFDLLFN TLCDNFLVKV DRASMLTLWK LEVHF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L77117 Genomic DNA. Translation: AAB99058.1. Different initiation.
PIRG64431.
RefSeqNP_248050.1. NC_000909.1.

3D structure databases

ProteinModelPortalQ58456.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243232.MJ1056.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB99058; AAB99058; MJ_1056.
GeneID1451953.
KEGGmja:MJ_1056.

Phylogenomic databases

eggNOGCOG0367.
KOK01953.
OMAYEATERC.
PhylomeDBQ58456.

Enzyme and pathway databases

UniPathwayUPA00134; UER00195.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMSSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01536. asn_synth_AEB. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASNH2_METJA
AccessionPrimary (citable) accession number: Q58456
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: September 26, 2003
Last modified: July 9, 2014
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names