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Protein

Putative asparagine synthetase [glutamine-hydrolyzing] 2

Gene

MJ1056

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway:iL-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Putative asparagine synthetase [glutamine-hydrolyzing] 1 (MJ1116), Putative asparagine synthetase [glutamine-hydrolyzing] 2 (MJ1056)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei114 – 1141GlutamineBy similarity
Binding sitei306 – 3061ATP; via amide nitrogen and carbonyl oxygenBy similarity
Sitei380 – 3801Important for beta-aspartyl-AMP intermediate formationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi378 – 3792ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative asparagine synthetase [glutamine-hydrolyzing] 2 (EC:6.3.5.4)
Gene namesi
Ordered Locus Names:MJ1056
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 515514Putative asparagine synthetase [glutamine-hydrolyzing] 2PRO_0000056938Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ_1056.

Structurei

3D structure databases

ProteinModelPortaliQ58456.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 229228Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni52 – 565Glutamine bindingBy similarity
Regioni92 – 943Glutamine bindingBy similarity

Sequence similaritiesi

Belongs to the asparagine synthetase family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0367.
InParanoidiQ58456.
KOiK01953.
OMAiDESIACV.
PhylomeDBiQ58456.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58456-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGINGIIRF GKEVIKEEIN KMNKAIKHRG PDDEGIFIYN FKNYSIGLGH
60 70 80 90 100
VRLAILDLSE KGHQPMGYNV DEDKIIYRDD ELDRADIIIV YNGEIYNYLE
110 120 130 140 150
LKEKFNLETE TGTDTEVILK LYNKLGFDCV KEFNGMWAFC IFDKKKGLIF
160 170 180 190 200
CSRDRLGVKP FYYYWDGNEF IFSSELKGIL AVKEINKKEN INKDAVELYF
210 220 230 240 250
ALGFIPSPYS IYKNTFKLEA RQNLIFDLDK REIRKYYYWE LPDYKPIYDK
260 270 280 290 300
KKLIEEGKKL LYDAVKIRMR SDVPVGAFLS GGLDSSTVVG VMREFTDLSK
310 320 330 340 350
LHTFSIGFEG KYDETPYIKI VVDYFKTQHH HYYFKERDFE ELIDKYSWIY
360 370 380 390 400
DEPFGDYSGF PTYKVSEMAR KFVTVVLSGD GGDEVFGGYM THLNGYRMDF
410 420 430 440 450
IRKLPKFLRV VGSKLPVKKD LNGIANLYLL KEAFRLSLIN PEEFYAESIK
460 470 480 490 500
EDAIRPEIYK KWTIEKLRYC LNKGDNKLGE ALRIFDLLFN TLCDNFLVKV
510
DRASMLTLWK LEVHF
Length:515
Mass (Da):60,473
Last modified:September 26, 2003 - v3
Checksum:iEE9EFBD4E643EDEB
GO

Sequence cautioni

The sequence AAB99058.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99058.1. Different initiation.
PIRiG64431.
RefSeqiNP_248050.1. NC_000909.1.
WP_010870569.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99058; AAB99058; MJ_1056.
GeneIDi1451953.
KEGGimja:MJ_1056.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99058.1. Different initiation.
PIRiG64431.
RefSeqiNP_248050.1. NC_000909.1.
WP_010870569.1. NC_000909.1.

3D structure databases

ProteinModelPortaliQ58456.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_1056.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99058; AAB99058; MJ_1056.
GeneIDi1451953.
KEGGimja:MJ_1056.

Phylogenomic databases

eggNOGiCOG0367.
InParanoidiQ58456.
KOiK01953.
OMAiDESIACV.
PhylomeDBiQ58456.

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiASNH2_METJA
AccessioniPrimary (citable) accession number: Q58456
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: September 26, 2003
Last modified: July 22, 2015
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.