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Q58456

- ASNH2_METJA

UniProt

Q58456 - ASNH2_METJA

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Protein

Putative asparagine synthetase [glutamine-hydrolyzing] 2

Gene

MJ1056

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei114 – 1141GlutamineBy similarity
Binding sitei306 – 3061ATP; via amide nitrogen and carbonyl oxygenBy similarity
Sitei380 – 3801Important for beta-aspartyl-AMP intermediate formationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi378 – 3792ATPBy similarity

GO - Molecular functioni

  1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative asparagine synthetase [glutamine-hydrolyzing] 2 (EC:6.3.5.4)
Gene namesi
Ordered Locus Names:MJ1056
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 515514Putative asparagine synthetase [glutamine-hydrolyzing] 2PRO_0000056938Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ1056.

Structurei

3D structure databases

ProteinModelPortaliQ58456.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 229228Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni52 – 565Glutamine bindingBy similarity
Regioni92 – 943Glutamine bindingBy similarity

Sequence similaritiesi

Belongs to the asparagine synthetase family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0367.
InParanoidiQ58456.
KOiK01953.
OMAiYEATERC.
PhylomeDBiQ58456.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58456-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGINGIIRF GKEVIKEEIN KMNKAIKHRG PDDEGIFIYN FKNYSIGLGH
60 70 80 90 100
VRLAILDLSE KGHQPMGYNV DEDKIIYRDD ELDRADIIIV YNGEIYNYLE
110 120 130 140 150
LKEKFNLETE TGTDTEVILK LYNKLGFDCV KEFNGMWAFC IFDKKKGLIF
160 170 180 190 200
CSRDRLGVKP FYYYWDGNEF IFSSELKGIL AVKEINKKEN INKDAVELYF
210 220 230 240 250
ALGFIPSPYS IYKNTFKLEA RQNLIFDLDK REIRKYYYWE LPDYKPIYDK
260 270 280 290 300
KKLIEEGKKL LYDAVKIRMR SDVPVGAFLS GGLDSSTVVG VMREFTDLSK
310 320 330 340 350
LHTFSIGFEG KYDETPYIKI VVDYFKTQHH HYYFKERDFE ELIDKYSWIY
360 370 380 390 400
DEPFGDYSGF PTYKVSEMAR KFVTVVLSGD GGDEVFGGYM THLNGYRMDF
410 420 430 440 450
IRKLPKFLRV VGSKLPVKKD LNGIANLYLL KEAFRLSLIN PEEFYAESIK
460 470 480 490 500
EDAIRPEIYK KWTIEKLRYC LNKGDNKLGE ALRIFDLLFN TLCDNFLVKV
510
DRASMLTLWK LEVHF
Length:515
Mass (Da):60,473
Last modified:September 26, 2003 - v3
Checksum:iEE9EFBD4E643EDEB
GO

Sequence cautioni

The sequence AAB99058.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99058.1. Different initiation.
PIRiG64431.
RefSeqiNP_248050.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99058; AAB99058; MJ_1056.
GeneIDi1451953.
KEGGimja:MJ_1056.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99058.1 . Different initiation.
PIRi G64431.
RefSeqi NP_248050.1. NC_000909.1.

3D structure databases

ProteinModelPortali Q58456.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJ1056.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB99058 ; AAB99058 ; MJ_1056 .
GeneIDi 1451953.
KEGGi mja:MJ_1056.

Phylogenomic databases

eggNOGi COG0367.
InParanoidi Q58456.
KOi K01953.
OMAi YEATERC.
PhylomeDBi Q58456.

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .

Family and domain databases

Gene3Di 3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiASNH2_METJA
AccessioniPrimary (citable) accession number: Q58456
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: September 26, 2003
Last modified: October 29, 2014
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3