Reviewed, UniProtKB/Swiss-Prot Q58446 (RPOA2_METJA)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase subunit A''
    EC=2.7.7.6
Cleaved into the following chain:
    1- Recommended name:
            Mja rpoA2 intein
        Alternative name(s):
            Mja rpol A'' intein

Gene names

Name:	rpoA2
Ordered Locus Names:	MJ1043

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_00411
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_00411
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the RNA polymerase beta' chain family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing Transcription
Cellular component	DNA-directed RNA polymerase
Ligand	Zinc
Molecular function	Endonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: HAMAP endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 75	75	DNA-directed RNA polymerase subunit A'', 1st part Potential		PRO_0000031065
	Chain	76 – 546	471	Mja rpoA2 intein Potential		PRO_0000031066
	Chain	547 – 859	313	DNA-directed RNA polymerase subunit A'', 2nd part Potential		PRO_0000031067
Regions
	Domain	248 – 380	133	DOD-type homing endonuclease

Sequences

	Sequence		Length	Mass (Da)
	Q58446-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: A05799E007899015	FASTA	859	97,087
	10 20 30 40 50 60 MDMEALKQKI EGLDIPQSLK DELFEKLSKE KDLTEEMVDE IIDEVVNAYR KALVEPYEAV 70 80 90 100 110 120 GIVAAQSIGE PGTQMSLPYE EKIIIKEGEF IKPVEIGKLV DEMIERFGFE KIGNSEVCDL 130 140 150 160 170 180 PIDIYALSLD QDEKVHWKRI ISCIRHKHNG KLIKIKTKSG REITATPYHS FVIRKDNKII 190 200 210 220 230 240 PVKGSELKIG DRIPVVKHIP ANCVEAINIS DYVSGNYVVD NINNKIAPKI NGKSIPNNIK 250 260 270 280 290 300 LDYDFGYFIG IYLAEGSVTK YFVSISNVDE LILNKIRAFA DKLGLNYGEY DNNNGFAESH 310 320 330 340 350 360 DIRIYSSTLA EFLSNFGTSS NTKKIAEFVF GANKEFVRGL IRGYFDGDGN VNADRKVIRV 370 380 390 400 410 420 TSNSKELIDG IAILLARFNI FSIKTKTKNQ FVLIIPHRYA KKFHEEINFS VEKKKSELER 430 440 450 460 470 480 LVSSLNDDKT YDSIDMIPSI GDALTKLGEK VDYPKVILKK FERKQKIGRA TLQRHLRRIE 490 500 510 520 530 540 ELAVKKGVNI LALKEYWLLK KAVESDVIWD EIVKIEEISC DKKYVYDISV EGLETFTTFD 550 560 570 580 590 600 GVLTHNTMRT FHYAGVAEIN VTLGLPRMIE IVDARKEPST PIMTIYLKEE YKDNREKAEE 610 620 630 640 650 660 IAKEIESLTL GSIAESISID LWTQSIKVEL DENRLADRGL TIDDVIEAIK KKLKVKIDVD 670 680 690 700 710 720 GTTLYLKIKT PSIKALRKRI PKIKNIQLKG IPGIERVLVK KEGGEYVLYT QGSNLREVFK 730 740 750 760 770 780 IDGVDTTRTI TNNIIEIQEV LGIEAARNAI INEMRNTLEQ QGLEVDIRHL MLVADIMTAD 790 800 810 820 830 840 GEVKPIGRHG VAGEKGSVLA RAAFEETVKH LYAAAERGDV DKLKGVIENV IVGKPIYLGT 850 GCVELTIDRE YEEGKNMEE

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB99047.1.
PIR	B64430.
RefSeq	NP_248037.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1451940.
GenomeReviews	Gene locus MJ1043 in contig L77117_GR.
KEGG	mja:MJ1043.
NMPDR	fig|243232.1.peg.1074.
TIGR	MJ1043.
Phylogenomic databases
HOGENOM	Q58446.
OMA	THNTMNT
Enzyme and pathway databases
BRENDA	2.7.7.6. 256362.
Family and domain databases
HAMAP	MF_00411. [Tree]
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR007081. RNA_pol_Rpb1_5. IPR012757. RpoA2. [Graphical view]
PANTHER	PTHR19376:SF13. RpoA2. 1 hit.
Pfam	PF04998. RNA_pol_Rpb1_5. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit. TIGR02389. RNA_pol_rpoA2. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOA2_METJA

Accession

Primary (citable) accession number: Q58446

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)