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Q58446

- RPOA2_METJA

UniProt

Q58446 - RPOA2_METJA

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Protein
DNA-directed RNA polymerase subunit A''
Gene
rpoA2, MJ1043
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity.UniRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. DNA-directed RNA polymerase activity Source: UniProtKB-KW
  3. endonuclease activity Source: UniProtKB-KW

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
  2. intron homing Source: UniProtKB-KW
  3. transcription, DNA-templated Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Intron homing, Transcription

Keywords - Ligandi

Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit A'' (EC:2.7.7.6)
Cleaved into the following chain:
Alternative name(s):
Mja rpol A'' intein
Gene namesi
Name:rpoA2
Ordered Locus Names:MJ1043
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7575DNA-directed RNA polymerase subunit A'', 1st part Reviewed prediction
PRO_0000031065Add
BLAST
Chaini76 – 546471Mja rpoA2 intein Reviewed prediction
PRO_0000031066Add
BLAST
Chaini547 – 859313DNA-directed RNA polymerase subunit A'', 2nd part Reviewed prediction
PRO_0000031067Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Reviewed prediction.UniRule annotation

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ1043.

Structurei

3D structure databases

ProteinModelPortaliQ58446.
SMRiQ58446. Positions 695-847.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini248 – 380133DOD-type homing endonuclease
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0086.
KOiK03042.
OMAiKVHWKRI.
PhylomeDBiQ58446.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 3 hits.
HAMAPiMF_00411. RNApol_arch_A.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR007081. RNA_pol_Rpb1_5.
IPR012757. RNAP_A2.
[Graphical view]
PfamiPF14528. LAGLIDADG_3. 1 hit.
PF04998. RNA_pol_Rpb1_5. 2 hits.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02389. RNA_pol_rpoA2. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58446-1 [UniParc]FASTAAdd to Basket

« Hide

MDMEALKQKI EGLDIPQSLK DELFEKLSKE KDLTEEMVDE IIDEVVNAYR    50
KALVEPYEAV GIVAAQSIGE PGTQMSLPYE EKIIIKEGEF IKPVEIGKLV 100
DEMIERFGFE KIGNSEVCDL PIDIYALSLD QDEKVHWKRI ISCIRHKHNG 150
KLIKIKTKSG REITATPYHS FVIRKDNKII PVKGSELKIG DRIPVVKHIP 200
ANCVEAINIS DYVSGNYVVD NINNKIAPKI NGKSIPNNIK LDYDFGYFIG 250
IYLAEGSVTK YFVSISNVDE LILNKIRAFA DKLGLNYGEY DNNNGFAESH 300
DIRIYSSTLA EFLSNFGTSS NTKKIAEFVF GANKEFVRGL IRGYFDGDGN 350
VNADRKVIRV TSNSKELIDG IAILLARFNI FSIKTKTKNQ FVLIIPHRYA 400
KKFHEEINFS VEKKKSELER LVSSLNDDKT YDSIDMIPSI GDALTKLGEK 450
VDYPKVILKK FERKQKIGRA TLQRHLRRIE ELAVKKGVNI LALKEYWLLK 500
KAVESDVIWD EIVKIEEISC DKKYVYDISV EGLETFTTFD GVLTHNTMRT 550
FHYAGVAEIN VTLGLPRMIE IVDARKEPST PIMTIYLKEE YKDNREKAEE 600
IAKEIESLTL GSIAESISID LWTQSIKVEL DENRLADRGL TIDDVIEAIK 650
KKLKVKIDVD GTTLYLKIKT PSIKALRKRI PKIKNIQLKG IPGIERVLVK 700
KEGGEYVLYT QGSNLREVFK IDGVDTTRTI TNNIIEIQEV LGIEAARNAI 750
INEMRNTLEQ QGLEVDIRHL MLVADIMTAD GEVKPIGRHG VAGEKGSVLA 800
RAAFEETVKH LYAAAERGDV DKLKGVIENV IVGKPIYLGT GCVELTIDRE 850
YEEGKNMEE 859
Length:859
Mass (Da):97,087
Last modified:November 1, 1997 - v1
Checksum:iA05799E007899015
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99047.1.
PIRiB64430.
RefSeqiNP_248037.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99047; AAB99047; MJ_1043.
GeneIDi1451940.
KEGGimja:MJ_1043.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99047.1 .
PIRi B64430.
RefSeqi NP_248037.1. NC_000909.1.

3D structure databases

ProteinModelPortali Q58446.
SMRi Q58446. Positions 695-847.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJ1043.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB99047 ; AAB99047 ; MJ_1043 .
GeneIDi 1451940.
KEGGi mja:MJ_1043.

Phylogenomic databases

eggNOGi COG0086.
KOi K03042.
OMAi KVHWKRI.
PhylomeDBi Q58446.

Family and domain databases

Gene3Di 2.170.16.10. 2 hits.
3.10.28.10. 3 hits.
HAMAPi MF_00411. RNApol_arch_A.
InterProi IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR007081. RNA_pol_Rpb1_5.
IPR012757. RNAP_A2.
[Graphical view ]
Pfami PF14528. LAGLIDADG_3. 1 hit.
PF04998. RNA_pol_Rpb1_5. 2 hits.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02389. RNA_pol_rpoA2. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiRPOA2_METJA
AccessioniPrimary (citable) accession number: Q58446
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi