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Q58446

- RPOA2_METJA

UniProt

Q58446 - RPOA2_METJA

Protein

DNA-directed RNA polymerase subunit A''

Gene

rpoA2

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.By similarity

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA-directed RNA polymerase activity Source: UniProtKB-KW
    3. endonuclease activity Source: UniProtKB-KW

    GO - Biological processi

    1. intein-mediated protein splicing Source: InterPro
    2. intron homing Source: UniProtKB-KW
    3. transcription, DNA-templated Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Intron homing, Transcription

    Keywords - Ligandi

    Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase subunit A'' (EC:2.7.7.6)
    Cleaved into the following chain:
    Alternative name(s):
    Mja rpol A'' intein
    Gene namesi
    Name:rpoA2
    Ordered Locus Names:MJ1043
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    Subcellular locationi

    Keywords - Cellular componenti

    DNA-directed RNA polymerase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7575DNA-directed RNA polymerase subunit A'', 1st partSequence AnalysisPRO_0000031065Add
    BLAST
    Chaini76 – 546471Mja rpoA2 inteinSequence AnalysisPRO_0000031066Add
    BLAST
    Chaini547 – 859313DNA-directed RNA polymerase subunit A'', 2nd partSequence AnalysisPRO_0000031067Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Protein-protein interaction databases

    STRINGi243232.MJ1043.

    Structurei

    3D structure databases

    ProteinModelPortaliQ58446.
    SMRiQ58446. Positions 695-847.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini248 – 380133DOD-type homing endonucleaseAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RNA polymerase beta' chain family.Curated

    Phylogenomic databases

    eggNOGiCOG0086.
    KOiK03042.
    OMAiKVHWKRI.
    PhylomeDBiQ58446.

    Family and domain databases

    Gene3Di2.170.16.10. 2 hits.
    3.10.28.10. 3 hits.
    HAMAPiMF_00411. RNApol_arch_A.
    InterProiIPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR007081. RNA_pol_Rpb1_5.
    IPR012757. RNAP_A2.
    [Graphical view]
    PfamiPF14528. LAGLIDADG_3. 1 hit.
    PF04998. RNA_pol_Rpb1_5. 2 hits.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view]
    SUPFAMiSSF51294. SSF51294. 2 hits.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR02389. RNA_pol_rpoA2. 1 hit.
    PROSITEiPS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q58446-1 [UniParc]FASTAAdd to Basket

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    MDMEALKQKI EGLDIPQSLK DELFEKLSKE KDLTEEMVDE IIDEVVNAYR    50
    KALVEPYEAV GIVAAQSIGE PGTQMSLPYE EKIIIKEGEF IKPVEIGKLV 100
    DEMIERFGFE KIGNSEVCDL PIDIYALSLD QDEKVHWKRI ISCIRHKHNG 150
    KLIKIKTKSG REITATPYHS FVIRKDNKII PVKGSELKIG DRIPVVKHIP 200
    ANCVEAINIS DYVSGNYVVD NINNKIAPKI NGKSIPNNIK LDYDFGYFIG 250
    IYLAEGSVTK YFVSISNVDE LILNKIRAFA DKLGLNYGEY DNNNGFAESH 300
    DIRIYSSTLA EFLSNFGTSS NTKKIAEFVF GANKEFVRGL IRGYFDGDGN 350
    VNADRKVIRV TSNSKELIDG IAILLARFNI FSIKTKTKNQ FVLIIPHRYA 400
    KKFHEEINFS VEKKKSELER LVSSLNDDKT YDSIDMIPSI GDALTKLGEK 450
    VDYPKVILKK FERKQKIGRA TLQRHLRRIE ELAVKKGVNI LALKEYWLLK 500
    KAVESDVIWD EIVKIEEISC DKKYVYDISV EGLETFTTFD GVLTHNTMRT 550
    FHYAGVAEIN VTLGLPRMIE IVDARKEPST PIMTIYLKEE YKDNREKAEE 600
    IAKEIESLTL GSIAESISID LWTQSIKVEL DENRLADRGL TIDDVIEAIK 650
    KKLKVKIDVD GTTLYLKIKT PSIKALRKRI PKIKNIQLKG IPGIERVLVK 700
    KEGGEYVLYT QGSNLREVFK IDGVDTTRTI TNNIIEIQEV LGIEAARNAI 750
    INEMRNTLEQ QGLEVDIRHL MLVADIMTAD GEVKPIGRHG VAGEKGSVLA 800
    RAAFEETVKH LYAAAERGDV DKLKGVIENV IVGKPIYLGT GCVELTIDRE 850
    YEEGKNMEE 859
    Length:859
    Mass (Da):97,087
    Last modified:November 1, 1997 - v1
    Checksum:iA05799E007899015
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99047.1.
    PIRiB64430.
    RefSeqiNP_248037.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB99047; AAB99047; MJ_1043.
    GeneIDi1451940.
    KEGGimja:MJ_1043.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99047.1 .
    PIRi B64430.
    RefSeqi NP_248037.1. NC_000909.1.

    3D structure databases

    ProteinModelPortali Q58446.
    SMRi Q58446. Positions 695-847.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ1043.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB99047 ; AAB99047 ; MJ_1043 .
    GeneIDi 1451940.
    KEGGi mja:MJ_1043.

    Phylogenomic databases

    eggNOGi COG0086.
    KOi K03042.
    OMAi KVHWKRI.
    PhylomeDBi Q58446.

    Family and domain databases

    Gene3Di 2.170.16.10. 2 hits.
    3.10.28.10. 3 hits.
    HAMAPi MF_00411. RNApol_arch_A.
    InterProi IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR007081. RNA_pol_Rpb1_5.
    IPR012757. RNAP_A2.
    [Graphical view ]
    Pfami PF14528. LAGLIDADG_3. 1 hit.
    PF04998. RNA_pol_Rpb1_5. 2 hits.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51294. SSF51294. 2 hits.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR02389. RNA_pol_rpoA2. 1 hit.
    PROSITEi PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

    Entry informationi

    Entry nameiRPOA2_METJA
    AccessioniPrimary (citable) accession number: Q58446
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3