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Q58446 (RPOA2_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit A''

EC=2.7.7.6

Cleaved into the following chain:

  1. Mja rpoA2 intein
    Alternative name(s):
    Mja rpol A'' intein
Gene names
Name:rpoA2
Ordered Locus Names:MJ1043
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus

Protein attributes

Sequence length859 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP-Rule MF_00411

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_00411

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_00411

Sequence similarities

Belongs to the RNA polymerase beta' chain family.

Contains 1 DOD-type homing endonuclease domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7575DNA-directed RNA polymerase subunit A'', 1st part Potential
PRO_0000031065
Chain76 – 546471Mja rpoA2 intein Potential
PRO_0000031066
Chain547 – 859313DNA-directed RNA polymerase subunit A'', 2nd part Potential
PRO_0000031067

Regions

Domain248 – 380133DOD-type homing endonuclease

Sequences

Sequence LengthMass (Da)Tools
Q58446 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: A05799E007899015

FASTA85997,087
        10         20         30         40         50         60 
MDMEALKQKI EGLDIPQSLK DELFEKLSKE KDLTEEMVDE IIDEVVNAYR KALVEPYEAV 

        70         80         90        100        110        120 
GIVAAQSIGE PGTQMSLPYE EKIIIKEGEF IKPVEIGKLV DEMIERFGFE KIGNSEVCDL 

       130        140        150        160        170        180 
PIDIYALSLD QDEKVHWKRI ISCIRHKHNG KLIKIKTKSG REITATPYHS FVIRKDNKII 

       190        200        210        220        230        240 
PVKGSELKIG DRIPVVKHIP ANCVEAINIS DYVSGNYVVD NINNKIAPKI NGKSIPNNIK 

       250        260        270        280        290        300 
LDYDFGYFIG IYLAEGSVTK YFVSISNVDE LILNKIRAFA DKLGLNYGEY DNNNGFAESH 

       310        320        330        340        350        360 
DIRIYSSTLA EFLSNFGTSS NTKKIAEFVF GANKEFVRGL IRGYFDGDGN VNADRKVIRV 

       370        380        390        400        410        420 
TSNSKELIDG IAILLARFNI FSIKTKTKNQ FVLIIPHRYA KKFHEEINFS VEKKKSELER 

       430        440        450        460        470        480 
LVSSLNDDKT YDSIDMIPSI GDALTKLGEK VDYPKVILKK FERKQKIGRA TLQRHLRRIE 

       490        500        510        520        530        540 
ELAVKKGVNI LALKEYWLLK KAVESDVIWD EIVKIEEISC DKKYVYDISV EGLETFTTFD 

       550        560        570        580        590        600 
GVLTHNTMRT FHYAGVAEIN VTLGLPRMIE IVDARKEPST PIMTIYLKEE YKDNREKAEE 

       610        620        630        640        650        660 
IAKEIESLTL GSIAESISID LWTQSIKVEL DENRLADRGL TIDDVIEAIK KKLKVKIDVD 

       670        680        690        700        710        720 
GTTLYLKIKT PSIKALRKRI PKIKNIQLKG IPGIERVLVK KEGGEYVLYT QGSNLREVFK 

       730        740        750        760        770        780 
IDGVDTTRTI TNNIIEIQEV LGIEAARNAI INEMRNTLEQ QGLEVDIRHL MLVADIMTAD 

       790        800        810        820        830        840 
GEVKPIGRHG VAGEKGSVLA RAAFEETVKH LYAAAERGDV DKLKGVIENV IVGKPIYLGT 

       850 
GCVELTIDRE YEEGKNMEE 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L77117 Genomic DNA. Translation: AAB99047.1.
PIRB64430.
RefSeqNP_248037.1. NC_000909.1.

3D structure databases

ProteinModelPortalQ58446.
SMRQ58446. Positions 695-847.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243232.MJ1043.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB99047; AAB99047; MJ_1043.
GeneID1451940.
KEGGmja:MJ_1043.

Phylogenomic databases

eggNOGCOG0086.
KOK03042.
OMAKVHWKRI.
ProtClustDBPRK14898.

Family and domain databases

Gene3D3.10.28.10. 3 hits.
HAMAPMF_00411. RNApol_arch_A.
InterProIPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR007081. RNA_pol_Rpb1_5.
IPR012757. RNAP_A2.
[Graphical view]
PfamPF14528. LAGLIDADG_3. 1 hit.
PF04998. RNA_pol_Rpb1_5. 2 hits.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF55608. SSF55608. 1 hit.
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02389. RNA_pol_rpoA2. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRPOA2_METJA
AccessionPrimary (citable) accession number: Q58446
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names

Intein-containing proteins

List of intein-containing protein entries