Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q58446

- RPOA2_METJA

UniProt

Q58446 - RPOA2_METJA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DNA-directed RNA polymerase subunit A''

Gene

rpoA2

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. DNA-directed RNA polymerase activity Source: UniProtKB-KW
  3. endonuclease activity Source: UniProtKB-KW

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
  2. intron homing Source: UniProtKB-KW
  3. transcription, DNA-templated Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Intron homing, Transcription

Keywords - Ligandi

Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit A'' (EC:2.7.7.6)
Cleaved into the following chain:
Alternative name(s):
Mja rpol A'' intein
Gene namesi
Name:rpoA2
Ordered Locus Names:MJ1043
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7575DNA-directed RNA polymerase subunit A'', 1st partSequence AnalysisPRO_0000031065Add
BLAST
Chaini76 – 546471Mja rpoA2 inteinSequence AnalysisPRO_0000031066Add
BLAST
Chaini547 – 859313DNA-directed RNA polymerase subunit A'', 2nd partSequence AnalysisPRO_0000031067Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ1043.

Structurei

3D structure databases

ProteinModelPortaliQ58446.
SMRiQ58446. Positions 695-847.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini248 – 380133DOD-type homing endonucleaseAdd
BLAST

Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.Curated

Phylogenomic databases

eggNOGiCOG0086.
InParanoidiQ58446.
KOiK03042.
OMAiKVHWKRI.
PhylomeDBiQ58446.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 3 hits.
HAMAPiMF_00411. RNApol_arch_A.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR007081. RNA_pol_Rpb1_5.
IPR012757. RNAP_A2.
[Graphical view]
PfamiPF14528. LAGLIDADG_3. 1 hit.
PF04998. RNA_pol_Rpb1_5. 2 hits.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02389. RNA_pol_rpoA2. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58446-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDMEALKQKI EGLDIPQSLK DELFEKLSKE KDLTEEMVDE IIDEVVNAYR
60 70 80 90 100
KALVEPYEAV GIVAAQSIGE PGTQMSLPYE EKIIIKEGEF IKPVEIGKLV
110 120 130 140 150
DEMIERFGFE KIGNSEVCDL PIDIYALSLD QDEKVHWKRI ISCIRHKHNG
160 170 180 190 200
KLIKIKTKSG REITATPYHS FVIRKDNKII PVKGSELKIG DRIPVVKHIP
210 220 230 240 250
ANCVEAINIS DYVSGNYVVD NINNKIAPKI NGKSIPNNIK LDYDFGYFIG
260 270 280 290 300
IYLAEGSVTK YFVSISNVDE LILNKIRAFA DKLGLNYGEY DNNNGFAESH
310 320 330 340 350
DIRIYSSTLA EFLSNFGTSS NTKKIAEFVF GANKEFVRGL IRGYFDGDGN
360 370 380 390 400
VNADRKVIRV TSNSKELIDG IAILLARFNI FSIKTKTKNQ FVLIIPHRYA
410 420 430 440 450
KKFHEEINFS VEKKKSELER LVSSLNDDKT YDSIDMIPSI GDALTKLGEK
460 470 480 490 500
VDYPKVILKK FERKQKIGRA TLQRHLRRIE ELAVKKGVNI LALKEYWLLK
510 520 530 540 550
KAVESDVIWD EIVKIEEISC DKKYVYDISV EGLETFTTFD GVLTHNTMRT
560 570 580 590 600
FHYAGVAEIN VTLGLPRMIE IVDARKEPST PIMTIYLKEE YKDNREKAEE
610 620 630 640 650
IAKEIESLTL GSIAESISID LWTQSIKVEL DENRLADRGL TIDDVIEAIK
660 670 680 690 700
KKLKVKIDVD GTTLYLKIKT PSIKALRKRI PKIKNIQLKG IPGIERVLVK
710 720 730 740 750
KEGGEYVLYT QGSNLREVFK IDGVDTTRTI TNNIIEIQEV LGIEAARNAI
760 770 780 790 800
INEMRNTLEQ QGLEVDIRHL MLVADIMTAD GEVKPIGRHG VAGEKGSVLA
810 820 830 840 850
RAAFEETVKH LYAAAERGDV DKLKGVIENV IVGKPIYLGT GCVELTIDRE

YEEGKNMEE
Length:859
Mass (Da):97,087
Last modified:November 1, 1997 - v1
Checksum:iA05799E007899015
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99047.1.
PIRiB64430.
RefSeqiNP_248037.1. NC_000909.1.
WP_010870556.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99047; AAB99047; MJ_1043.
GeneIDi1451940.
KEGGimja:MJ_1043.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99047.1 .
PIRi B64430.
RefSeqi NP_248037.1. NC_000909.1.
WP_010870556.1. NC_000909.1.

3D structure databases

ProteinModelPortali Q58446.
SMRi Q58446. Positions 695-847.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJ1043.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB99047 ; AAB99047 ; MJ_1043 .
GeneIDi 1451940.
KEGGi mja:MJ_1043.

Phylogenomic databases

eggNOGi COG0086.
InParanoidi Q58446.
KOi K03042.
OMAi KVHWKRI.
PhylomeDBi Q58446.

Family and domain databases

Gene3Di 2.170.16.10. 2 hits.
3.10.28.10. 3 hits.
HAMAPi MF_00411. RNApol_arch_A.
InterProi IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR007081. RNA_pol_Rpb1_5.
IPR012757. RNAP_A2.
[Graphical view ]
Pfami PF14528. LAGLIDADG_3. 1 hit.
PF04998. RNA_pol_Rpb1_5. 2 hits.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02389. RNA_pol_rpoA2. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiRPOA2_METJA
AccessioniPrimary (citable) accession number: Q58446
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3