Reviewed, UniProtKB/Swiss-Prot Q58445 (RPOA1_METJA)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase subunit A'
    EC=2.7.7.6
Cleaved into the following chain:
    1- Recommended name:
            Mja rpoA1 intein
        Alternative name(s):
            Mja rpol A' intein

Gene names

Name:	rpoA1
Ordered Locus Names:	MJ1042

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	1341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Cofactor	Zinc.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the RNA polymerase beta' chain family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	DNA-directed RNA polymerase subunit A', 1st part Potential		PRO_0000031062
	Chain	461 – 911	451	Mja rpoA1 intein Potential		PRO_0000031063
	Chain	912 – 1341	430	DNA-directed RNA polymerase subunit A', 2nd part Potential		PRO_0000031064
Regions
	Domain	616 – 783	168	DOD-type homing endonuclease
	Zinc finger	62 – 105	44	C4-type By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58445-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 4D98546ED552AF12	FASTA	1,341	152,781
	10 20 30 40 50 60 MERYEIPKEI GEIMFGLLSP DYIRQMSVAK IVTPDTYDED GYPIDGGLMD TRLGVIDPGL 70 80 90 100 110 120 VCKTCGGRIG ECPGHFGHIE LAKPVIHIGF AKTIYKILKA VCPHCGRVAI SETKRKEILE 130 140 150 160 170 180 KMEKLERDGG NKWEVCEEVY KEASKVTICP HCGEIKYDIK FEKPTTYYRI DGNEEKTLTP 190 200 210 220 230 240 SDVREILEKI PDEDCILLGL NPEVARPEWM VLTVLPVPPV TVRPSITLET GERSEDDLTH 250 260 270 280 290 300 KLVDIIRINN RLEENIEGGA PNLIIEDLWN LLQYHVNTYF DNEAPGIPPA KHRSGRPLKT 310 320 330 340 350 360 LAQRLKGKEG RFRYNLAGKR VNFSSRTVIS PDPCLSINEV GVPEVVAKEL TVPEKVTKYN 370 380 390 400 410 420 IERIRQLLRN GSEKHPGVNY VIRKMIGRDG TEQEYKVKIT ESNKDFWAEN IREGDIVERH 430 440 450 460 470 480 LMDGDIVLYN RQPSLHRMSI MAHRVRVLPY RTFRHNLCVC VDGDTTVLLD GKLIKIKDLE 490 500 510 520 530 540 DKWKDVKVLT SDDLNPKLTS LSKYWKLNAD EYGKKIYKIK TELGREIIAT EDHPFYTTNG 550 560 570 580 590 600 RKRCGELKVG DEVIIYPNDF PMFEDDNRVI VDEEKIKKVI NNIGGTYKNK IINELKDRKL 610 620 630 640 650 660 IPLTYNDQKA SILARIVGHV MGDGSLIINN KNSRVVFRGD IEDLKTIKED LKELGYDGEE 670 680 690 700 710 720 IKLHEGETEI TDYNGKKRII KGKGYSFEVR KKSLCILLKA LGCVGGDKTK KMYGIPNWIK 730 740 750 760 770 780 TAPKYIKKEF LSAYFGSELT TPKIRNHGTS FKELSFKIAK IEEIFDEDRF IKDIKEMLKE 790 800 810 820 830 840 FGIELKVRVE EGNLRKDGYK TKVYVASIYN HKEFFGRIGY TYANKKETLA RYAYEYLLTK 850 860 870 880 890 900 EKYLKDRNIK KLENNTKFIT FDKFIKEKCL KNGFVKEKIV SIEETKVDYV YDITTISETH 910 920 930 940 950 960 NFIANGFLTG NCPPYNADFD GDEMNLHVPQ SEEARAEAEA LMLVEKHILS PRFGGPIIGA 970 980 990 1000 1010 1020 IHDFISGAYL LTSNYFTKDE ATLILRSGGI KDELWEPDKV ENGVPLYSGK KIFSKALPKG 1030 1040 1050 1060 1070 1080 LNLRYKAKIC RKCDVCKKEE CEYDAYVVIK DGELIKGVID KNGYGAEAGL ILHTIVKEFG 1090 1100 1110 1120 1130 1140 PEAGRKFLDS ATKMAIRAVM LRGFTTGIDD EDLPEEALKE IEKVLDEAEE KVKEIIEKYE 1150 1160 1170 1180 1190 1200 RGELELLPGL NLEESREAYI SNVLREARDK AGAIAERYLG LDNHAVIMAV TGARGNILNL 1210 1220 1230 1240 1250 1260 TQMAACLGQQ SVRGKRIFRG YRGRVLPHFE KGDLGARSHG FVRSSYKKGL SPTEFFFHAM 1270 1280 1290 1300 1310 1320 GGREGLVDQA VRTAQSGYMQ RRLINALQDL KTEFDGTVRD SRGIMIQFKY GEDGIDPMLA 1330 1340 DRGKAVNIDR IIDKVKMKYN Q

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB99046.1.
RefSeq	NP_248036.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1451939.
GenomeReviews	Gene locus MJ1042 in contig L77117_GR.
KEGG	mja:MJ1042.
NMPDR	fig|243232.1.peg.1073.
TIGR	MJ1042.
Phylogenomic databases
HOGENOM	Q58445.
OMA	IGWTVER
Enzyme and pathway databases
BRENDA	2.7.7.6. 256362.
Family and domain databases
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. IPR012758. RpoA1. [Graphical view]
Gene3D	G3DSA:3.90.1120.10. RNA_pol_Rpb1_1. 1 hit.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 2 hits. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. SM00663. RPOLA_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit. TIGR02390. RNA_pol_rpoA1. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOA1_METJA

Accession

Primary (citable) accession number: Q58445

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	November 24, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)