Q58444 (RPOB2_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit B''
EC=2.7.7.6

Gene names

Name:	rpoB2
Ordered Locus Names:	MJ1040

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Cofactor	Zinc.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Ligand	Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	DNA-directed RNA polymerase subunit B''		PRO_0000074034

Sequences

	Sequence		Length	Mass (Da)
	Q58444 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: F57FD38EEEB79298	FASTA	498	56,487
	10 20 30 40 50 60 MAIMRELVDA YFKEHGLIDH QIESYNDFVE NRLQKIIDEV GYIETEITGG YKVKLGKIKV 70 80 90 100 110 120 GKPVIKEADG SIRPITPMEA RIRDLTYSVP LYLEMTPIIG EGEDAREGET VEVYIGELPV 130 140 150 160 170 180 MLGSKICHLY GKSREELIDL GEDPEDPFGY FIINGTEKVL ITQEDLIPNR ILCEKAERSG 190 200 210 220 230 240 KIVDVAKVFS TRHGFRALCT VERHPDGLLY ATFPGMPGQI PLVILMKALG AETDKDIIES 250 260 270 280 290 300 IDDERFFMEI VLNIQEIREE HNINSPEDAL EFIGKRVAPG QAKDYRLKRA ETVLCNYLLP 310 320 330 340 350 360 HLGVTKEDFP KKIRFLGIMA RNALELYFGY RGEDDKDHYA YKRAKLAGDL MEDLFRYAFS 370 380 390 400 410 420 QLVKDIKYQL ERQTLRNKTP SIQAAVRSDI LTERIKHAMA TGTWVGGKTG VSQLLDRTSY 430 440 450 460 470 480 LATNSQLRRI VSPLSRSQPH FEARELHGTH WGKICPSETP EGPNCGLVKN FAIMCKVTRE 490 EDDSKVIELL KSFGINVS

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99043.1.
PIR	G64429.
RefSeq	NP_248034.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58444.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1451937.
GenomeReviews	Gene locus MJ1040 in contig L77117_GR.
KEGG	mja:MJ_1040.
NMPDR	fig|243232.1.peg.1071.
TIGR	MJ1040.
Phylogenomic databases
HOGENOM	HBG496841.
OMA	LAGDLME.
ProtClustDB	PRK09606.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1040-MONOMER.
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. [Graphical view]
KO	K03045.
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB2_METJA

Accession

Primary (citable) accession number: Q58444

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 16, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program