Q58443 (RPOH_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit H
EC=2.7.7.6

Gene names

Name:	rpoH
Ordered Locus Names:	MJ1039

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	78 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_00025
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_00025
Sequence similarities	Belongs to the archaeal rpoH/eukaryotic RPB5 RNA polymerase subunit family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 78	78	DNA-directed RNA polymerase subunit H HAMAP MF_00025		PRO_0000146092

Sequences

	Sequence		Length	Mass (Da)
	Q58443 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: CDF2DAF342E6D41B	FASTA	78	9,001
	10 20 30 40 50 60 MKVTDHILVP KHEIVPKEEV EEILKRYNIK IQQLPKIYED DPVIQEIGAK EGDVVRVIRK 70 SPTAGVSIAY RLVIKRII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"RNA polymerase subunit H features a beta-ribbon motif within a novel fold that is present in archaea and eukaryotes." Thiru A., Hodach M., Eloranta J.J., Kostourou V., Weinzierl R.O., Matthews S. J. Mol. Biol. 287:753-760(1999) [PubMed: 10191143] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L77117 Genomic DNA. Translation: AAB99042.1.

PIR

F64429.

RefSeq

NP_248033.1. NC_000909.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HMJ	NMR	-	A	1-78	[»]

ProteinModelPortal

Q58443.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1451936.

GenomeReviews

Gene locus MJ1039 in contig L77117_GR.

KEGG

mja:MJ_1039.

NMPDR

fig|243232.1.peg.1070.

TIGR

MJ1039.

Phylogenomic databases

HOGENOM

HBG496846.

OMA

EHELVPK.

ProtClustDB

PRK09570.

Enzyme and pathway databases

BioCyc

MJAN243232:MJ_1039-MONOMER.

Family and domain databases

HAMAP

MF_00025. RNApol_arch_H.
[Tree]

InterPro

IPR020609. DNA-dir_RNA_pol_subH/Rpb5_arc.
IPR000783. RNA_pol_subH/Rpb5_C.
IPR020608. RNA_pol_subH/Rpb5_CS.
[Graphical view]

Gene3D

G3DSA:3.90.940.20. RNApol_RPB5. 1 hit.

KO

K03053.

Pfam

PF01191. RNA_pol_Rpb5_C. 1 hit.
[Graphical view]

ProDom

PD005155. RNA_pol_subH/Rpb5_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF55287. RNApol_RPB5_like. 1 hit.

PROSITE

PS01110. RNA_POL_H_23KD. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RPOH_METJA

Accession

Primary (citable) accession number: Q58443

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 16, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program