Reviewed, UniProtKB/Swiss-Prot Q58425 (CARA_METJA)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	MJ1019

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112358
Regions
	Domain	171 – 354	184	Glutamine amidotransferase type-1
	Region	1 – 171	171	CPSase HAMAP MF_01209
Sites
	Active site	246	1	Nucleophile By similarity
	Active site	330	1	By similarity
	Active site	332	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58425-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 4D8F07776CA65F9F	FASTA	354	39,920
	10 20 30 40 50 60 MEAVLILEDG TILKGKGFGA EKEVFGELVF TTVMTGYVEV LTDPSYKGQI VMMTYPLEGN 70 80 90 100 110 120 YGVKKDWFES DGIKAEGFVV REVTSKALDD FLKEYDIPGI QDIDTRFLTR KIRDKGVVKS 130 140 150 160 170 180 CLKVAEEISD DEISELLERV KRYSDISDID LVPLVSTKEP KIHKTANPKA RCVLIDCGVK 190 200 210 220 230 240 LNIIRSLVQR NCEVIQVPYN TKYDEILEYK PDFVLISNGP GDPARLKEVI KNIKNLIGVV 250 260 270 280 290 300 PITGICLGNQ LLSLAFGGET YKMKFGHRGG NQPVKDLKTQ KVYITSQNHG FAVRKESLPD 310 320 330 340 350 DVEVSFINLN DMTVEGIRHK DLPIFSVQFH PEARPGPHDT MFLFDEMIKL KDRK

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB99021.1.
RefSeq	NP_248013.1.
3D structure databases
HSSP	HSSP built from PDB template 1CS0 based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	1451916.
GenomeReviews	Gene locus MJ1019 in contig L77117_GR.
KEGG	mja:MJ1019.
NMPDR	fig|243232.1.peg.1050.
TIGR	MJ1019.
Phylogenomic databases
HOGENOM	Q58425.
OMA	Q58425. LFDGSNC.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1019-MON.
BRENDA	6.3.5.5. 256362.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_METJA

Accession

Primary (citable) accession number: Q58425

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)