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Protein

Carbamoyl-phosphate synthase small chain

Gene

carA

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain, N-terminal section (carB1), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain, C-terminal section (carB2)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain, N-terminal section (carB1), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain, C-terminal section (carB2)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei246NucleophileBy similarity1
Active sitei330By similarity1
Active sitei332By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Protein family/group databases

MEROPSiC26.A33.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase small chain (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain
Gene namesi
Name:carA
Ordered Locus Names:MJ1019
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001123581 – 354Carbamoyl-phosphate synthase small chainAdd BLAST354

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.By similarity

Protein-protein interaction databases

STRINGi243232.MJ_1019.

Structurei

3D structure databases

ProteinModelPortaliQ58425.
SMRiQ58425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini171 – 354Glutamine amidotransferase type-1Add BLAST184

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 171CPSaseAdd BLAST171

Sequence similaritiesi

Belongs to the CarA family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00064. Archaea.
COG0505. LUCA.
InParanoidiQ58425.
KOiK01956.
OMAiCFNTGMT.
OrthoDBiPOG093Z085N.
PhylomeDBiQ58425.

Family and domain databases

CDDicd01744. GATase1_CPSase. 1 hit.
Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiView protein in InterPro
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR035686. CPSase_GATase1.
IPR017926. GATASE.
PfamiView protein in Pfam
PF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
SMARTiView protein in SMART
SM01097. CPSase_sm_chain. 1 hit.
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiView protein in PROSITE
PS51273. GATASE_TYPE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q58425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAVLILEDG TILKGKGFGA EKEVFGELVF TTVMTGYVEV LTDPSYKGQI
60 70 80 90 100
VMMTYPLEGN YGVKKDWFES DGIKAEGFVV REVTSKALDD FLKEYDIPGI
110 120 130 140 150
QDIDTRFLTR KIRDKGVVKS CLKVAEEISD DEISELLERV KRYSDISDID
160 170 180 190 200
LVPLVSTKEP KIHKTANPKA RCVLIDCGVK LNIIRSLVQR NCEVIQVPYN
210 220 230 240 250
TKYDEILEYK PDFVLISNGP GDPARLKEVI KNIKNLIGVV PITGICLGNQ
260 270 280 290 300
LLSLAFGGET YKMKFGHRGG NQPVKDLKTQ KVYITSQNHG FAVRKESLPD
310 320 330 340 350
DVEVSFINLN DMTVEGIRHK DLPIFSVQFH PEARPGPHDT MFLFDEMIKL

KDRK
Length:354
Mass (Da):39,920
Last modified:December 15, 1998 - v2
Checksum:i4D8F07776CA65F9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99021.1.
RefSeqiWP_010870532.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99021; AAB99021; MJ_1019.
GeneIDi1451916.
KEGGimja:MJ_1019.

Similar proteinsi

Entry informationi

Entry nameiCARA_METJA
AccessioniPrimary (citable) accession number: Q58425
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: August 30, 2017
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families