Q58414 (ILVE_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative branched-chain-amino-acid aminotransferase
Short name=BCAT
EC=2.6.1.42

Alternative name(s):
Transaminase B

Gene names

Name:	ilvE
Ordered Locus Names:	MJ1008

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts on leucine, isoleucine and valine By similarity.
Catalytic activity	L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate. 2-oxoglutaric acid + L-isoleucine = (S)-3-methyl-2-oxopentanoic acid + L-glutamic acid. 2-oxoglutaric acid + L-valine = 3-methyl-2-oxobutanoic acid + L-glutamic acid.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4. Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.
Sequence similarities	Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-isoleucine transaminase activity Inferred from electronic annotation. Source: EC L-leucine transaminase activity Inferred from electronic annotation. Source: EC L-valine transaminase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Putative branched-chain-amino-acid aminotransferase		PRO_0000103290
Amino acid modifications
	Modified residue	146	1	N6-(pyridoxal phosphate)lysine Potential

Sequences

	Sequence		Length	Mass (Da)
	Q58414 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 033DB417DFD89CBC	FASTA	288	32,096
	10 20 30 40 50 60 MKIYLNGKFV DEKDAKVSVF DHGLLYGDGV FEGIRAYDGV VFMLKEHIDR LYDSAKSLCI 70 80 90 100 110 120 DIPLTKEEMI DVVLETLRVN NLRDAYIRLV VTRGVGDLGL DPRKCGKPTI FCIAIPMPPL 130 140 150 160 170 180 LGEDGIRAIT VSVRRLPVDV LNPAVKSLNY LNSVLAKIQA NYAGVDEAFL LDDKGFVVEG 190 200 210 220 230 240 TGDNIFIVKN GVLKTPPVYQ SILKGITRDV VIKLAKEEGI EVVEEPLTLH DLYTADELFI 250 260 270 280 TGTAAEIVPV FEIDGRVINN KQVGEITKKL KEKFKDIRTK WGIKVYDE

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99010.1.
PIR	G64425.
RefSeq	NP_248002.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58414.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1451905.
GenomeReviews	Gene locus MJ1008 in contig L77117_GR.
KEGG	mja:MJ_1008.
NMPDR	fig|243232.1.peg.1039.
TIGR	MJ1008.
Phylogenomic databases
HOGENOM	HBG617123.
OMA	IQANYAG.
ProtClustDB	PRK08320.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1008-MONOMER.
Family and domain databases
InterPro	IPR001544. Aminotrans_IV. IPR018300. Aminotrans_IV_CS. IPR005785. B_amino_transI. [Graphical view]
KO	K00826.
PANTHER	PTHR11825. Aminotrans_IV. 1 hit.
Pfam	PF01063. Aminotran_4. 1 hit. [Graphical view]
SUPFAM	SSF56752. Aminotrans_IV. 1 hit.
TIGRFAMs	TIGR01122. IlvE_I. 1 hit.
PROSITE	PS00770. AA_TRANSFER_CLASS_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVE_METJA

Accession

Primary (citable) accession number: Q58414

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program