Q58413 (SYV_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Valine--tRNA ligase
EC=6.1.1.9

Alternative name(s):
Valyl-tRNA synthetase
Short name=ValRS

Gene names

Name:	valS
Ordered Locus Names:	MJ1007

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity. HAMAP MF_02005
Catalytic activity	ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP MF_02005
Subcellular location	Cytoplasm By similarity HAMAP MF_02005.
Domain	ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. HAMAP MF_02005
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	valyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW valine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 878	878	Valine--tRNA ligase HAMAP MF_02005		PRO_0000106249
Regions
	Motif	43 – 53	11	"HIGH" region HAMAP MF_02005
	Motif	527 – 531	5	"KMSKS" region HAMAP MF_02005
Sites
	Binding site	530	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58413 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4D78BCA0D1F2022E	FASTA	878	102,572
	10 20 30 40 50 60 MEMPKDYNIE IEKQIQKKWE ESKIYKFDEE SNKPPYIIDT PPPYPTGRLH LGHALNWTYM 70 80 90 100 110 120 DIIARYKRMK GFNVLFPQGW DCHGLPTEVK VEEIHGITKS DVDRHKFREL CIELTKENIE 130 140 150 160 170 180 KMRRQIKSLG ISIDWDKEYI TMTPEYIKKS QTAFVRMYKD GLIYRGKFPV NWCPRCQTAI 190 200 210 220 230 240 AFAEVEYKER ESKLNYIKFP AADGEGHLLI ATTRPELMAA CVAILVHPED ERYKHLIGKE 250 260 270 280 290 300 FIVPLFGHKV KLLADEDVEK EFGTGAVMVC TFGDKTDVLW VNRHKLEIKK AIDEKGELTE 310 320 330 340 350 360 IAGKYKGLKT EEAREKIIED LKKEGYLVKQ EPIKQNVGVC WRCKTPIEII VTEQWFVNVR 370 380 390 400 410 420 KLIPKVREVA DEIKWIPEHM KIRLLNWIED MDWDWVISRQ RIFATPIPVW YCPKCGNVVV 430 440 450 460 470 480 AKEEDLPIDP TKTGYVCDKC GNKDLIPETD VLDTWMDSSI TPMVITKWLD DDKFFEKHYP 490 500 510 520 530 540 VQLRPQGHDI IRTWAFYTIV KSVALTGKKP WDEIVINGMV FGEDGHKMSK SRGNVVEPDE 550 560 570 580 590 600 IIAKYGADAL RLWASNSVVG DDVQFLWKEV DYGYRFLRKS WNACRFAKMH ISDDIIDELK 610 620 630 640 650 660 KPMEISNPID LWILSKLQRL IERVDKDLEN YRFNTIVEIY KFVWHEFCDN YIEMVKYRLY 670 680 690 700 710 720 GDDEEAKKEA RWTLYYVIDK VVRLLCPFAP HFSDYIAEIY KIDNLHFSFP EVDNRFINEE 730 740 750 760 770 780 AEKFGEIAKN TVISIRRFKA NSGMALNAPL KYVEIYTEDE ETYLALNKTA EDIKGTLKIE 790 800 810 820 830 840 ELKIIKGKPA LESKIVEIIP DKSKIGPEFK KNAKAVMDLI KNADEETLEK IINEGLETEY 850 860 870 GVIRKEHIKD VKRALFCEGE EVDSVDIEGV LAMAIIRK

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99009.1.
PIR	F64425.
RefSeq	NP_248001.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58413.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1451904.
GenomeReviews	Gene locus MJ1007 in contig L77117_GR.
KEGG	mja:MJ_1007.
NMPDR	fig|243232.1.peg.1038.
TIGR	MJ1007.
Phylogenomic databases
HOGENOM	HBG577712.
OMA	HMGHALV.
ProtClustDB	PRK13208.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1007-MONOMER.
Family and domain databases
HAMAP	MF_02005. Val_tRNA_synth_type2. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. IPR002303. Valyl-tRNA_synthetase. IPR022874. Valyl-tRNA_synthetase_type_2. [Graphical view]
Gene3D	G3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01873.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00986. TRNASYNTHVAL.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00422. ValS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYV_METJA

Accession

Primary (citable) accession number: Q58413

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program