Reviewed, UniProtKB/Swiss-Prot Q58392 (MTM1_METJA)

Names and origin

Protein names

Recommended name:
    Modification methylase MjaI
      Short name=M.MjaI
    EC=2.1.1.113
Alternative name(s):
    N-4 cytosine-specific methyltransferase MjaI

Gene names

Name:	mjaIM
Ordered Locus Names:	MJ0985

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CTAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MjaI endonuclease Potential.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation on cytosine Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Modification methylase MjaI		PRO_0000087936

Sequences

	Sequence		Length	Mass (Da)
	Q58392-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6D4DB9B36EA90E80	FASTA	303	35,518
	10 20 30 40 50 60 MMSIDITTNH KIIFGDARKM DEIEDESVHL VVTSPPYPMI EMWDELFKML NLEINKRWME 70 80 90 100 110 120 MENEEDEEKK EKLIMQIYNL MHQTLYPVWE EVYRVLVPGG IACINIGDAT RKINGVFRLF 130 140 150 160 170 180 PNHSKIIENF EKIGFVTLPY ILWKKPSNKP NAFLGSGFLP PNAYVTLDVE YILIFRKGKP 190 200 210 220 230 240 RKFKPKDPLR YASAYTKEER DRWFSQIWEI VGDKQTHPKI ERRTASFPEE IPRRLIRMFS 250 260 270 280 290 300 IIGDTVLDPF LGTGTTVKAA IELKRNSIGY EIDKSLKPII EEKIGIKQKR IGMDFNVEFI NRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Method for screening restriction endonucleases." Noren C.J., Roberts R.J., Patti J., Byrd D.R., Morgan R.D. Patent number WO9911821, 11-MAR-1999 Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98988.1.
PIR	A64423.
RefSeq	NP_247980.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3889. M.MjaI.
Genome annotation databases
GeneID	1451883.
GenomeReviews	Gene locus MJ0985 in contig L77117_GR.
KEGG	mja:MJ0985.
NMPDR	fig|243232.1.peg.1017.
TIGR	MJ0985.
Phylogenomic databases
HOGENOM	HBG405684.
OMA	MIEMWDE.
Enzyme and pathway databases
BRENDA	2.1.1.113. 256362.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR001091. MeTrfase_CN4. IPR017985. MeTrfase_CN4_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTM1_METJA

Accession

Primary (citable) accession number: Q58392

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)