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Q58391 (T2M1_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme MjaI

Short name=R.MjaI
EC=3.1.21.4
Alternative name(s):
Endonuclease MjaI
Type II restriction enzyme MjaI
Gene names
Name:mjaIR
Ordered Locus Names:MJ0984
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus

Protein attributes

Sequence length222 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence CTAG and cleaves after C-1 By similarity.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 222222Type-2 restriction enzyme MjaI
PRO_0000077332

Sequences

Sequence LengthMass (Da)Tools
Q58391 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9A254A208AB8AC02

FASTA22225,962
        10         20         30         40         50         60 
MVKLMKKLED KKGIIEITFE EEREILELPS KPELPKYASQ LINLANIFSQ GTRPKVVGQM 

        70         80         90        100        110        120 
SELIKEFRKT GGRTFEDWKK WYLQKYPNAI DEATEKIWNM LNNFKETLEQ LERDDVRKWV 

       130        140        150        160        170        180 
EDLVLIKTYE GLMLQDAILK KVAEELGGNY RPSTIEEESK GIDGVIIIDD KEIPVSIKSK 

       190        200        210        220 
TYVNQEKHLS EELKGHLIIY EKKKNKIIVD YSDLLDLVEN TK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L77117 Genomic DNA. Translation: AAB98987.1.
PIRH64422.
RefSeqNP_247979.1. NC_000909.1.

3D structure databases

ProteinModelPortalQ58391.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243232.MJ0984.

Protein family/group databases

REBASE1221. MjaI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB98987; AAB98987; MJ_0984.
GeneID1451882.
KEGGmja:MJ_0984.

Phylogenomic databases

eggNOGNOG238082.
OMAQMSELIK.

Family and domain databases

InterProIPR019068. Restrct_endonuc_II_MjaI.
[Graphical view]
PfamPF09568. RE_MjaI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2M1_METJA
AccessionPrimary (citable) accession number: Q58391
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names