Q58391 (T2M1_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme MjaI
Short name=R.MjaI
EC=3.1.21.4

Alternative name(s):
Endonuclease MjaI
Type II restriction enzyme MjaI

Gene names

Name:	mjaIR
Ordered Locus Names:	MJ0984

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes the double-stranded sequence CTAG and cleaves after C-1 By similarity.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Type-2 restriction enzyme MjaI		PRO_0000077332

Sequences

	Sequence		Length	Mass (Da)
	Q58391 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9A254A208AB8AC02	FASTA	222	25,962
	10 20 30 40 50 60 MVKLMKKLED KKGIIEITFE EEREILELPS KPELPKYASQ LINLANIFSQ GTRPKVVGQM 70 80 90 100 110 120 SELIKEFRKT GGRTFEDWKK WYLQKYPNAI DEATEKIWNM LNNFKETLEQ LERDDVRKWV 130 140 150 160 170 180 EDLVLIKTYE GLMLQDAILK KVAEELGGNY RPSTIEEESK GIDGVIIIDD KEIPVSIKSK 190 200 210 220 TYVNQEKHLS EELKGHLIIY EKKKNKIIVD YSDLLDLVEN TK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Method for screening restriction endonucleases." Noren C.J., Roberts R.J., Patti J., Byrd D.R., Morgan R.D. Patent number WO9911821, 11-MAR-1999 Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98987.1.
PIR	H64422.
RefSeq	NP_247979.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58391.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJ0984.
Protein family/group databases
REBASE	1221. MjaI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB98987; AAB98987; MJ_0984.
GeneID	1451882.
KEGG	mja:MJ_0984.
Phylogenomic databases
eggNOG	NOG238082.
OMA	QMSELIK.
ProtClustDB	CLSK539695.
Family and domain databases
InterPro	IPR019068. Restrct_endonuc_II_MjaI. [Graphical view]
Pfam	PF09568. RE_MjaI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2M1_METJA

Accession

Primary (citable) accession number: Q58391

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program