Reviewed, UniProtKB/Swiss-Prot Q58391 (T2M1_METJA)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme MjaI
      Short name=R.MjaI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme MjaI
    Endonuclease MjaI

Gene names

Name:	mjaIR
Ordered Locus Names:	MJ0984

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes the double-stranded sequence CTAG and cleaves after C-1 By similarity.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Type-2 restriction enzyme MjaI		PRO_0000077332

Sequences

	Sequence		Length	Mass (Da)
	Q58391-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9A254A208AB8AC02	FASTA	222	25,962
	10 20 30 40 50 60 MVKLMKKLED KKGIIEITFE EEREILELPS KPELPKYASQ LINLANIFSQ GTRPKVVGQM 70 80 90 100 110 120 SELIKEFRKT GGRTFEDWKK WYLQKYPNAI DEATEKIWNM LNNFKETLEQ LERDDVRKWV 130 140 150 160 170 180 EDLVLIKTYE GLMLQDAILK KVAEELGGNY RPSTIEEESK GIDGVIIIDD KEIPVSIKSK 190 200 210 220 TYVNQEKHLS EELKGHLIIY EKKKNKIIVD YSDLLDLVEN TK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Method for screening restriction endonucleases." Noren C.J., Roberts R.J., Patti J., Byrd D.R., Morgan R.D. Patent number WO9911821, 11-MAR-1999 Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98987.1.
PIR	H64422.
RefSeq	NP_247979.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1451882.
GenomeReviews	Gene locus MJ0984 in contig L77117_GR.
KEGG	mja:MJ0984.
NMPDR	fig|243232.1.peg.1016.
TIGR	MJ0984.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0984-MON.
BRENDA	3.1.21.4. 256362.
Family and domain databases
InterPro	IPR019068. Restrict_endonuc_II_MjaI. [Graphical view]
Pfam	PF09568. RE_MjaI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2M1_METJA

Accession

Primary (citable) accession number: Q58391

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	July 28, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)