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Protein

Type-2 restriction enzyme MjaI

Gene

mjaIR

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence CTAG and cleaves after C-1.By similarity

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1221. MjaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme MjaI (EC:3.1.21.4)
Short name:
R.MjaI
Alternative name(s):
Endonuclease MjaI
Type II restriction enzyme MjaI
Gene namesi
Name:mjaIR
Ordered Locus Names:MJ0984
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 222222Type-2 restriction enzyme MjaIPRO_0000077332Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ0984.

Structurei

3D structure databases

ProteinModelPortaliQ58391.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG238082.
InParanoidiQ58391.
OMAiQMSELIK.

Family and domain databases

InterProiIPR019068. Restrct_endonuc_II_MjaI.
[Graphical view]
PfamiPF09568. RE_MjaI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q58391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKLMKKLED KKGIIEITFE EEREILELPS KPELPKYASQ LINLANIFSQ
60 70 80 90 100
GTRPKVVGQM SELIKEFRKT GGRTFEDWKK WYLQKYPNAI DEATEKIWNM
110 120 130 140 150
LNNFKETLEQ LERDDVRKWV EDLVLIKTYE GLMLQDAILK KVAEELGGNY
160 170 180 190 200
RPSTIEEESK GIDGVIIIDD KEIPVSIKSK TYVNQEKHLS EELKGHLIIY
210 220
EKKKNKIIVD YSDLLDLVEN TK
Length:222
Mass (Da):25,962
Last modified:November 1, 1996 - v1
Checksum:i9A254A208AB8AC02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98987.1.
PIRiH64422.
RefSeqiNP_247979.1. NC_000909.1.
WP_010870498.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98987; AAB98987; MJ_0984.
GeneIDi1451882.
KEGGimja:MJ_0984.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98987.1.
PIRiH64422.
RefSeqiNP_247979.1. NC_000909.1.
WP_010870498.1. NC_000909.1.

3D structure databases

ProteinModelPortaliQ58391.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ0984.

Protein family/group databases

REBASEi1221. MjaI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB98987; AAB98987; MJ_0984.
GeneIDi1451882.
KEGGimja:MJ_0984.

Phylogenomic databases

eggNOGiNOG238082.
InParanoidiQ58391.
OMAiQMSELIK.

Family and domain databases

InterProiIPR019068. Restrct_endonuc_II_MjaI.
[Graphical view]
PfamiPF09568. RE_MjaI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  2. Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiT2M1_METJA
AccessioniPrimary (citable) accession number: Q58391
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.