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Q58372 (RNP4_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease P protein component 4

Short name=RNase P component 4
EC=3.1.26.5
Alternative name(s):
Rpp21
Gene names
Name:rnp4
Ordered Locus Names:MJ0962
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus

Protein attributes

Sequence length128 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Ref.2 Ref.3 Ref.4

Catalytic activity

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor. HAMAP-Rule MF_00757

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00757

Subunit structure

Consists of a catalytic RNA component and at least 4 protein subunits. Forms a subcomplex with Rnp1 which stimulates the catalytic RNA. Ref.2 Ref.3 Ref.4

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00757.

Sequence similarities

Belongs to the eukaryotic/archaeal RNase P protein component 4 family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandMetal-binding
Zinc
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA 5'-leader removal

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

ribonuclease P complex

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionribonuclease P activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 128128Ribonuclease P protein component 4 HAMAP-Rule MF_00757
PRO_0000153852

Sites

Metal binding631Zinc By similarity
Metal binding661Zinc By similarity
Metal binding921Zinc By similarity
Metal binding951Zinc By similarity

Sequences

Sequence LengthMass (Da)Tools
Q58372 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 462D677CE5B159D3

FASTA12815,567
        10         20         30         40         50         60 
MKKFLEKKLK KIAYERIDIL MSLAEEEAKK GNWDRAKRYV YLARRIAMKM RIRFPKKWKR 

        70         80         90        100        110        120 
RICKKCGTFL LYGRNARVRI KSKRYPHVVI TCLECGAIYR IPMIREKKEK RRKKLEERLK 


AKSNSQTS 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii."
Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. expand/collapse author list , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]"Studies on Methanocaldococcus jannaschii RNase P reveal insights into the roles of RNA and protein cofactors in RNase P catalysis."
Pulukkunat D.K., Gopalan V.
Nucleic Acids Res. 36:4172-4180(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH RNP1, SUBUNIT.
Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[3]"Cooperative RNP assembly: complementary rescue of structural defects by protein and RNA subunits of archaeal RNase P."
Chen W.Y., Xu Y., Cho I.M., Oruganti S.V., Foster M.P., Gopalan V.
J. Mol. Biol. 411:368-383(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH RNP1, SUBUNIT.
Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[4]"Fidelity of tRNA 5'-maturation: a possible basis for the functional dependence of archaeal and eukaryal RNase P on multiple protein cofactors."
Chen W.Y., Singh D., Lai L.B., Stiffler M.A., Lai H.D., Foster M.P., Gopalan V.
Nucleic Acids Res. 40:4666-4680(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH RNP1, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L77117 Genomic DNA. Translation: AAB98964.1.
PIRB64420.
RefSeqNP_247957.1. NC_000909.1.

3D structure databases

ProteinModelPortalQ58372.
SMRQ58372. Positions 6-104.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243232.MJ0962.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB98964; AAB98964; MJ_0962.
GeneID1451860.
KEGGmja:MJ_0962.

Phylogenomic databases

eggNOGCOG2023.
KOK03540.
OMAISINDCT.
PhylomeDBQ58372.

Family and domain databases

HAMAPMF_00757. RNase_P_4.
InterProIPR016432. RNase_P_comp-4.
IPR007175. Rpr2.
[Graphical view]
PANTHERPTHR14742. PTHR14742. 1 hit.
PfamPF04032. Rpr2. 1 hit.
[Graphical view]
PIRSFPIRSF004878. RNase_P_4. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRNP4_METJA
AccessionPrimary (citable) accession number: Q58372
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names