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Q58357

- SYI_METJA

UniProt

Q58357 - SYI_METJA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei603 – 6031ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MJ0947
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10391039Isoleucine--tRNA ligasePRO_0000098580Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243232.MJ0947.

Structurei

3D structure databases

ProteinModelPortaliQ58357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 5611"HIGH" regionAdd
BLAST
Motifi600 – 6045"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
InParanoidiQ58357.
KOiK01870.
OMAiKPVHWCL.
PhylomeDBiQ58357.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q58357-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKVEPVNFR ELDKKIKKFW EENDIYQKVK KKNERNKEFY FVDGPPYCSG
60 70 80 90 100
AIHLGTAWNK IIKDTYLRFK RMQGYNVLDK AGWDMHGLPI EVKVENEFGI
110 120 130 140 150
KNKKEIETKI GVKQFIEKCK EFALKHKEIM EKQFKNLGVW LDWENAYMPI
160 170 180 190 200
TKEYMEIGWW TLKVAHEKGL LTRDLRVVYW CPRCETALAE HEVRGEYKEV
210 220 230 240 250
YDPSVYVKFR LANEENTYIV IWTTTPWTLV ANLAVTVHPD YDYAYVEVEF
260 270 280 290 300
DDKKEVWIIA EKLVEEVINK AKKFHNIKNY KIIKKVKGKE LEGIKYIHPL
310 320 330 340 350
LEENERQKEF AELENAHTVI LGEHVTLEGG TGLVHTAPGH GEEDFEVGKK
360 370 380 390 400
YNLPIYSPID DEGKYVEGKW KGVFVKDADA EIIETLKNKG LLVYAGKIKH
410 420 430 440 450
SYPHCWRCKT PLLFRATEQW FLEISKIKDN IIEHAKTVQW IPHWVETRYI
460 470 480 490 500
NGVKFVGDWN ISRQRYWGIP IPVWVCEKCG KYIVVGSVEE LEEKMINKDE
510 520 530 540 550
VGEINDLHKP TVDKIKLRCE CGGEMKRVPD VLDVWFDSGL APYASIGVKE
560 570 580 590 600
LKKADFITEG HDQVTKWFYS QHALSAIVFN DIPYKKCLMH GFTLDEHGDK
610 620 630 640 650
MSKSLGNVVN PDDVVEKYGA DLLRFYLLSA NKVWEDLRFV WSEMDDVLSL
660 670 680 690 700
FNTLWNAYMF AVNYMVLDNF KPDEKYFEYL KDEDRWIVSR INSVAKIAIE
710 720 730 740 750
NLEVPYFHTY TWTLKDFILN DLSRWYIRLI RDRTWKEKDD ADKLAAYQTL
760 770 780 790 800
YYVLLKLATI LAPVAPHTAE AIYQNLKTED MEESIFMNKI EVDEEFIDEE
810 820 830 840 850
LERDMAIVRD VVDAIYRGRD RIKYTLRYPL KEITIAGGEE VKKAVERFEY
860 870 880 890 900
IIKEQGNVKN IKFGEVEGSK YIIKPNYREL GKRYRSEVPK VVEALNKADA
910 920 930 940 950
KELMERLKEG AVILDGYEIK PEYVEIRLEI PEHIAGVEFS KGTVFINTEI
960 970 980 990 1000
TDDLIKEGLM REVIRRIQAM RKDMDLDIEE KIKIKVEGID LDEFKEIIER
1010 1020 1030
EVRGQFVDEI KADYEKDWEI KTPNGEKYNV KIAIERINK
Length:1,039
Mass (Da):122,234
Last modified:November 1, 1996 - v1
Checksum:i7163C46B4AE08AC0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB98949.1.
PIRiC64418.
RefSeqiNP_247942.1. NC_000909.1.
WP_010870461.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98949; AAB98949; MJ_0947.
GeneIDi1451844.
KEGGimja:MJ_0947.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB98949.1 .
PIRi C64418.
RefSeqi NP_247942.1. NC_000909.1.
WP_010870461.1. NC_000909.1.

3D structure databases

ProteinModelPortali Q58357.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJ0947.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB98949 ; AAB98949 ; MJ_0947 .
GeneIDi 1451844.
KEGGi mja:MJ_0947.

Phylogenomic databases

eggNOGi COG0060.
InParanoidi Q58357.
KOi K01870.
OMAi KPVHWCL.
PhylomeDBi Q58357.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiSYI_METJA
AccessioniPrimary (citable) accession number: Q58357
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3