Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q58339 (PUR8_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenylosuccinate lyase
Short name=ASL
EC=4.3.2.2
Alternative name(s):
Adenylosuccinase
Short name=ASase
Gene names
Name:purB
Ordered Locus Names:MJ0929
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus ›

Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

N(6)-(1,2-dicarboxyethyl)AMP = fumarate + AMP.

(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

Pathway

Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.

Subunit structure

Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site By similarity.

Sequence similarities

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 462462Adenylosuccinate lyase
PRO_0000137888

Regions

Region21 – 222Substrate binding By similarity
Region87 – 893Substrate binding By similarity

Sites

Active site1621Proton donor/acceptor By similarity
Active site2871Proton donor/acceptor By similarity
Binding site2361Substrate By similarity
Binding site3261Substrate By similarity
Binding site3311Substrate By similarity
Binding site3351Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q58339 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D0E89A0D2F0C1894

FASTA46252,592
        10         20         30         40         50         60 
MELNQIIKSG EKMAVHPIDY RYGTPEMRKV WEEENKLEKM LKVEAALAKA QAELGLIPKE 

        70         80         90        100        110        120 
AAEEINKKAS TKYVKLERVK EIEKQTKHDV VAMIRALAEV CEGNAGEYIH FGATSNDIVD 

       130        140        150        160        170        180 
TANSLLIKES IEIIEDKLKQ LRDILLDKAE EHKYTVCVGR THGQHAIPTT YGMRFALWAA 

       190        200        210        220        230        240 
EIDRHLERLK EAKKRICVSM ITGAVGTMAA MGEKGLEVHK RVAEILGLEP VLISNQVIQR 

       250        260        270        280        290        300 
DRHAEFVFLL ALIAQTLNKI GVTVRSMQRT EIGELEEEFD PTKQTGSSTM PHKRNPITFE 

       310        320        330        340        350        360 
QICGLSRVIK SLCIAEMDNI PLWEERDLTN SSAERCIFAE VCVLTDHILT LAIKGVKKLK 

       370        380        390        400        410        420 
VNKENVERNL ELTKGLIMAE RIMIELAKRG MGRQTAHEIV RQCAMKAYEE KRHLKDILLE 

       430        440        450        460 
NEEVMKYITK EELEELMNPK TYIGLAPQIV DEVIKTLKNK KY

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L77117 Genomic DNA. Translation: AAB98931.1.
PIRA64416.
RefSeqNP_247924.1. NC_000909.1.

3D structure databases

ProteinModelPortalQ58339.
ModBaseSearch...

Protein-protein interaction databases

STRING243232.MJ0929.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB98931; AAB98931; MJ_0929.
GeneID1451818.
KEGGmja:MJ_0929.

Phylogenomic databases

eggNOGCOG0015.
KOK01756.
OMAERIMIEL.
ProtClustDBPRK08540.

Enzyme and pathway databases

UniPathwayUPA00074; UER00132.
UPA00075; UER00336.

Family and domain databases

Gene3D1.10.275.10. 1 hit.
InterProIPR019468. AdenyloSucc_lyase_C.
IPR003031. D_crystallin.
IPR024083. Fumarase/histidase_N.
IPR000362. Fumarate_lyase.
IPR020557. Fumarate_lyase_CS.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
IPR004769. Pur_lyase.
[Graphical view]
PANTHERPTHR11444:SF2. PTHR11444:SF2. 1 hit.
PfamPF10397. ADSL_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSPR00145. ARGSUCLYASE.
PR00149. FUMRATELYASE.
SMARTSM00998. ADSL_C. 1 hit.
[Graphical view]
SUPFAMSSF48557. L-Aspartase-like. 1 hit.
TIGRFAMsTIGR00928. purB. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR8_METJA
AccessionPrimary (citable) accession number: Q58339
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents