Q58327 (PPNK_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	MJ0917

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP-Rule MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361
Cofactor	Divalent metal ions By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the C-terminal section; belongs to the NAD kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NAD metabolic process Inferred from electronic annotation. Source: InterPro NADP biosynthetic process Inferred from electronic annotation. Source: InterPro lysine metabolic process Inferred from electronic annotation. Source: InterPro phosphatidylinositol phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 574	574	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP-Rule MF_00361		PRO_0000120700
Regions
	Region	302 – 574	273	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP-Rule MF_00361

Sequences

	Sequence		Length	Mass (Da)
	Q58327 [UniParc]. Last modified June 1, 1998. Version 2. Checksum: 89A1AF944BD99DB1	FASTA	574	64,119
	10 20 30 40 50 60 MVIMEGFKIA MKVIDEIDKK IKPLIGWEKA DEVVKIGADG TPTKRIDVIA ENMAINILEK 70 80 90 100 110 120 FSGGILISEE IGLKVVGDEL EYIFILDPID GTYNALKSIP IYSTSIAVAK IKGEDKKLIR 130 140 150 160 170 180 ENINNIDWIK SFIANKYTIN DLYVGIVKNL ATGDLYYAIK GEGSFLEKDG EKIKIETKNI 190 200 210 220 230 240 KDLKEASVGL FVYGLSNDLL EFLKERKVRR VRLFGSMALE MCYVVSGALD AYINVNENSR 250 260 270 280 290 300 LCDIAGAYVI CREGNAIVTN KNGKPLNMKL HLMERTSLIV SNKYLHKKLI ALFGNRWIIK 310 320 330 340 350 360 PVKFGIVVRE DKEEAINLAI EICKYLKDKN IPFCVEDFLR ERVGGDKFDI SAISHIIAIG 370 380 390 400 410 420 GDGTILRASR LVNGETIPII AVNMGKVGFL AEFCKDEVFE IIDKVIYGEY EIEKRSKLSC 430 440 450 460 470 480 KIIKDNRVIK TPSALNEMVV ITKNPAKILE FDVYVNDTLV ENVRADGIIV STPTGSTAYS 490 500 510 520 530 540 LSAGGPIVEP NVDCFIISPI CPFKLSSRPL VISASNRIKL KLKLEKPALL VIDGSVEYEI 550 560 570 NKDDELIFEK SDSYAYFVKG QSFYNKLSRC LGIK

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98922.1.
PIR	E64414.
RefSeq	NP_247912.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58327.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJ0917.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB98922; AAB98922; MJ_0917.
GeneID	1451806.
KEGG	mja:MJ_0917.
Phylogenomic databases
eggNOG	COG0061.
KO	K00858.
OMA	PLIGWEK.
ProtClustDB	PRK14076.
Family and domain databases
Gene3D	2.60.200.30. 1 hit. 3.40.50.10330. 1 hit.
HAMAP	MF_00361. NAD_kinase. Fused.
InterPro	IPR017438. ATP-NAD_kinase_dom_1. IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR021175. Bifunctional_PpnK_predicted. IPR020583. Inositol_monoP_metal-BS. IPR000760. Inositol_monophosphatase. IPR002504. PolyP/ATP_NADK_prd. [Graphical view]
PANTHER	PTHR20275. PTHR20275. 1 hit.
Pfam	PF00459. Inositol_P. 2 hits. PF01513. NAD_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF036641. Bifunctional_PpnK_predicted. 1 hit.
PRINTS	PR00377. IMPHPHTASES.
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
PROSITE	PS00629. IMP_1. 1 hit. Uncertain. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPNK_METJA

Accession

Primary (citable) accession number: Q58327

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program