Q58323 (COABC_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coenzyme A biosynthesis bifunctional protein CoaBC

Alternative name(s):
DNA/pantothenate metabolism flavoprotein

Including the following 2 domains:

1. Phosphopantothenoylcysteine decarboxylase
   Short name=PPCDC
   EC=4.1.1.36
   Alternative name(s):
   CoaC
2. Phosphopantothenate--cysteine ligase
   EC=6.3.2.5
   Alternative name(s):
   CoaB
   PPC synthetase
   Short name=PPCS
   Phosphopantothenoylcysteine synthase

Gene names

Name:	coaBC
Ordered Locus Names:	MJ0913

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine By similarity.
Catalytic activity	N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO2. CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-((R)-4'-phosphopantothenoyl)-L-cysteine.
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 2/5. Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 3/5.
Subunit structure	Homododecamer, the CoaB domains form homodimers.
Sequence similarities	In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily. In the C-terminal section; belongs to the PPC synthetase family.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Decarboxylase Ligase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	coenzyme A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway pantothenate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	FMN binding Inferred from electronic annotation. Source: InterPro phosphopantothenate--cysteine ligase activity Inferred from electronic annotation. Source: EC phosphopantothenoylcysteine decarboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Coenzyme A biosynthesis bifunctional protein CoaBC		PRO_0000232696
Regions
	Region	1 – 195	195	Phosphopantothenoylcysteine decarboxylase
	Region	196 – 403	208	Phosphopantothenate--cysteine ligase

Sequences

	Sequence		Length	Mass (Da)
	Q58323 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B8B51AA81A115B94	FASTA	403	45,671
	10 20 30 40 50 60 MISEIMHPTK LLKGTKSKLL ENKKILVAVT SSIAAIETPK LMRELIRHGA EVYCIITEET 70 80 90 100 110 120 KKIIGKEALK FGCGNEVYEE ITGDIEHILL YNECDCLLIY PATANIISKI NLGIADNIVN 130 140 150 160 170 180 TTALMFFGNK PIFIVPAMHE NMFNAIKRHI DKLKEKDKIY IISPKFEEGK AKVANIEDVV 190 200 210 220 230 240 KAVIEKIGNN LKKEGNRVLI LNGGTVEFID KVRVISNLSS GKMGVALAEA FCKEGFYVEV 250 260 270 280 290 300 ITAMGLEPPY YIKNHKVLTA KEMLNKAIEL AKDFDIIISS AAISDFTVES FEGKLSSEEE 310 320 330 340 350 360 LILKLKRNPK VLEELRRIYK DKVIIGFKAE YNLDEKELIN RAKERLNKYN LNMIIANDLS 370 380 390 400 KHYFGDDYIE VYIITKYEVE KISGSKKEIS ERIVEKVKKL VKS

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98918.1.
PIR	A64414.
RefSeq	NP_247908.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58323.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJ0913.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB98918; AAB98918; MJ_0913.
GeneID	1451802.
KEGG	mja:MJ_0913.
Phylogenomic databases
eggNOG	COG0452.
KO	K13038.
OMA	VTSGPTH.
ProtClustDB	CLSK876384.
Enzyme and pathway databases
UniPathway	UPA00241; UER00353. UPA00241; UER00354.
Family and domain databases
Gene3D	3.40.50.10300. 1 hit. 3.40.50.1950. 1 hit.
InterPro	IPR005252. CoaBC. IPR007085. DNA/pantothenate-metab_flavo_C. IPR003382. Flavoprotein. [Graphical view]
Pfam	PF04127. DFP. 1 hit. PF02441. Flavoprotein. 1 hit. [Graphical view]
SUPFAM	SSF102645. DNA/pantothenate-metab_flavo_C. 1 hit. SSF52507. Flavoprotein. 1 hit.
TIGRFAMs	TIGR00521. coaBC_dfp. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COABC_METJA

Accession

Primary (citable) accession number: Q58323

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program