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Protein

2-phospho-L-lactate guanylyltransferase

Gene

cofC

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Guanylyltransferase that catalyzes the activation of 2-phospho-L-lactate (LP) as (2S)-lactyl-2-diphospho-5'-guanosine (LPPG), via the condensation of LP with GTP. Is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor. Is able to utilize other purine nucleotides including dGTP and ITP as cosubstrates in place of GTP but with a lower activity. Does not display lactate kinase activity.1 Publication

Catalytic activityi

(2S)-2-phospholactate + GTP = (2S)-lactyl-2-diphospho-5'-guanosine + diphosphate.1 Publication

Kineticsi

  1. KM=36 µM for (2S)-2-phospholactate1 Publication
  2. KM=56 µM for GTP1 Publication
  1. Vmax=3 µmol/min/mg enzyme1 Publication

Temperature dependencei

Retains all activity when heated up to 90 degrees Celsius for 10 minutes and retained 38% activity after 10 minutes at 100 degrees Celsius.1 Publication

Pathwayi: coenzyme F420 biosynthesis

This protein is involved in the pathway coenzyme F420 biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway coenzyme F420 biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • phospholactate guanylyltransferase activity Source: UniProtKB

GO - Biological processi

  • coenzyme biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14615.
BRENDAi2.7.7.68. 3260.
SABIO-RKQ58297.
UniPathwayiUPA00071.

Names & Taxonomyi

Protein namesi
Recommended name:
2-phospho-L-lactate guanylyltransferase (EC:2.7.7.68)
Short name:
LP guanylyltransferase
Gene namesi
Name:cofC
Ordered Locus Names:MJ0887
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001070911 – 2242-phospho-L-lactate guanylyltransferaseAdd BLAST224

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi243232.MJ_0887.

Structurei

3D structure databases

ProteinModelPortaliQ58297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CofC family.Curated

Phylogenomic databases

eggNOGiarCOG04472. Archaea.
COG1920. LUCA.
InParanoidiQ58297.
KOiK14941.
OMAiRAVIPYK.
PhylomeDBiQ58297.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_02114. CofC. 1 hit.
InterProiIPR002835. Coenzyme_F420_CofC.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01983. CofC. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03552. F420_cofC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q58297-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNCGIKMKVI IPVSPINSLK TRLSEFLSGE ERKNLLLNML KDIIKALDGL
60 70 80 90 100
DIVIVSRDEE ILDFAKNELK AETIKEKYKG LNNAIKQAFE EIEDKEVIII
110 120 130 140 150
PADIPLIKKK HIEDILKLSK NYDLIIAPSR GGGTNLLYLK SKDLIEIKYE
160 170 180 190 200
GFSFLKHLEE AKKRNLRYYI YDSFLISVDI NTPEDLGEIF IHGNDTYTKN
210 220
YLKSLGIDVE PKHSSAGRFV VKRR
Length:224
Mass (Da):25,683
Last modified:November 1, 1996 - v1
Checksum:i0565EDD51F313278
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98891.1.
PIRiG64410.

Genome annotation databases

EnsemblBacteriaiAAB98891; AAB98891; MJ_0887.
KEGGimja:MJ_0887.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98891.1.
PIRiG64410.

3D structure databases

ProteinModelPortaliQ58297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_0887.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB98891; AAB98891; MJ_0887.
KEGGimja:MJ_0887.

Phylogenomic databases

eggNOGiarCOG04472. Archaea.
COG1920. LUCA.
InParanoidiQ58297.
KOiK14941.
OMAiRAVIPYK.
PhylomeDBiQ58297.

Enzyme and pathway databases

UniPathwayiUPA00071.
BioCyciMetaCyc:MONOMER-14615.
BRENDAi2.7.7.68. 3260.
SABIO-RKQ58297.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_02114. CofC. 1 hit.
InterProiIPR002835. Coenzyme_F420_CofC.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01983. CofC. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03552. F420_cofC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOFC_METJA
AccessioniPrimary (citable) accession number: Q58297
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 11, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.