Q58295 (DPOL_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase
EC=2.7.7.7

Cleaved into the following 2 chains:

1. Mja pol-1 intein
2. Mja pol-2 intein

Gene names

Name:	pol
Ordered Locus Names:	MJ0885

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	1634 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the DNA polymerase type-B family. Contains 2 DOD-type homing endonuclease domains.

Ontologies

Keywords
Biological process	DNA replication
Domain	Repeat
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	DNA polymerase, 1st part Potential		PRO_0000007317
	Chain	426 – 794	369	Mja pol-1 intein Potential		PRO_0000007318
	Chain	795 – 882	88	DNA polymerase, 2nd part Potential		PRO_0000007319
	Chain	883 – 1358	476	Mja pol-2 intein Potential		PRO_0000007320
	Chain	1359 – 1634	276	DNA polymerase, 3rd part Potential		PRO_0000007321
Regions
	Domain	552 – 693	142	DOD-type homing endonuclease 1
	Domain	1163 – 1295	133	DOD-type homing endonuclease 2

Sequences

	Sequence		Length	Mass (Da)
	Q58295 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 84A1FAFAB1F97DDD	FASTA	1,634	191,710
	10 20 30 40 50 60 MGMSMGKIKI DALIDNTYKT IEDKAVIYLY LINSILKDRD FKPYFYVELH KEKVENEDIE 70 80 90 100 110 120 KIKEFLLKND LLKFVENIEV VKKIILRKEK EVIKIIATHP QKVPKLRKIK ECEIVKEIYE 130 140 150 160 170 180 HDIPFAKRYL IDNEIIPMTY WDFENKKPVS IEIPKLKSVA FDMEVYNRDT EPNPERDPIL 190 200 210 220 230 240 MASFWDENGG KVITYKEFNH PNIEVVKNEK ELIKKIIETL KEYDVIYTYN GDNFDFPYLK 250 260 270 280 290 300 ARAKIYGIDI NLGKDGEELK IKRGGMEYRS YIPGRVHIDL YPISRRLLKL TKYTLEDVVY 310 320 330 340 350 360 NLFGIEKLKI PHTKIVDYWA NNDKTLIEYS LQDAKYTYKI GKYFFPLEVM FSRIVNQTPF 370 380 390 400 410 420 EITRMSSGQM VEYLLMKRAF KENMIVPNKP DEEEYRRRVL TTYEGGYVKE PEKGMFEDII 430 440 450 460 470 480 SMDFRCHPKG TKVVVKGKGI VNIEDVKEGN YVLGIDGWQK VKKVWKYEYE GELINVNGLK 490 500 510 520 530 540 CTPNHKIPLR YKIKHKKINK NDYLVRDIYA KSLLTKFKGE GKLILCKDFE TIGNYEKYIN 550 560 570 580 590 600 DMDEDFILKS ELIGILLAEG HLLRRDIEYF DSSRGKKRIS HQYRVEITVN EDEKDFIEKI 610 620 630 640 650 660 KYIFKKLFNY ELYVRRKKGT KAITLGCAKK DIYLKIEEIL KNKEKYLPNA ILRGFFEGDG 670 680 690 700 710 720 YVNTVRRAVV VNQGTNNYDK IKFIASLLDR LGIKYSFYTY SYEERGKKLK RYVIEIFSKG 730 740 750 760 770 780 DLIKFSILIS FISRRKNNLL NEIIRQKTLY KIGDYGFYDL DDVCVSLESY KGEVYDLTLE 790 800 810 820 830 840 GRPYYFANGI LTHNSLYPSI IISYNISPDT LDCECCKDVS EKILGHWFCK KKEGLIPKTL 850 860 870 880 890 900 RNLIERRINI KRRMKKMAEI GEINEEYNLL DYEQKSLKIL ANSILPDEYL TIIEEDGIKV 910 920 930 940 950 960 VKIGEYIDDL MRKHKDKIKF SGISEILETK NLKTFSFDKI TKKCEIKKVK ALIRHPYFGK 970 980 990 1000 1010 1020 AYKIKLRSGR TIKVTRGHSL FKYENGKIVE VKGDDVRFGD LIVVPKKLTC VDKEVVINIP 1030 1040 1050 1060 1070 1080 KRLINADEEE IKDLVITKHK DKAFFVKLKK TLEDIENNKL KVIFDDCILY LKELGLIDYN 1090 1100 1110 1120 1130 1140 IIKKINKVDI KILDEEKFKA YKKYFDTVIE HGNFKKGRCN IQYIKIKDYI ANIPDKEFED 1150 1160 1170 1180 1190 1200 CEIGAYSGKI NALLKLDEKL AKFLGFFVTR GRLKKQKLKG ETVYEISVYK SLPEYQKEIA 1210 1220 1230 1240 1250 1260 ETFKEVFGAG SMVKDKVTMD NKIVYLVLKY IFKCGDKDKK HIPEELFLAS ESVIKSFLDG 1270 1280 1290 1300 1310 1320 FLKAKKNSHK GTSTFMAKDE KYLNQLMILF NLVGIPTRFT PVKNKGYKLT LNPKYGTVKD 1330 1340 1350 1360 1370 1380 LMLDEVKEIE AFEYSGYVYD LSVEDNENFL VNNIYAHNSV YGYLAFPRAR FYSRECAEIV 1390 1400 1410 1420 1430 1440 TYLGRKYILE TVKEAEKFGF KVLYIDTDGF YAIWKEKISK EELIKKAMEF VEYINSKLPG 1450 1460 1470 1480 1490 1500 TMELEFEGYF KRGIFVTKKR YALIDENGRV TVKGLEFVRR DWSNIAKITQ RRVLEALLVE 1510 1520 1530 1540 1550 1560 GSIEKAKKII QDVIKDLREK KIKKEDLIIY TQLTKDPKEY KTTAPHVEIA KKLMREGKRI 1570 1580 1590 1600 1610 1620 KVGDIIGYII VKGTKSISER AKLPEEVDID DIDVNYYIDN QILPPVLRIM EAVGVSKNEL 1630 KKEGAQLTLD KFFK

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98889.1.
PIR	E64410.
RefSeq	NP_247880.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58295.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJ0885.
Protein family/group databases
MEROPS	N10.007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB98889; AAB98889; MJ_0885.
GeneID	1451774.
KEGG	mja:MJ_0885.
Phylogenomic databases
eggNOG	COG0417.
KO	K02319.
OMA	NIYAHNS.
ProtClustDB	CLSK2517665.
Family and domain databases
InterPro	IPR006172. DNA-dir_DNA_pol_B. IPR017964. DNA-dir_DNA_pol_B_CS. IPR006133. DNA-dir_DNA_pol_B_exonuc. IPR006134. DNA-dir_DNA_pol_B_multi_dom. IPR004578. DNA-dir_DNA_pol_B_pol2. IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR012337. RNaseH-like_dom. [Graphical view]
Pfam	PF00136. DNA_pol_B. 3 hits. PF03104. DNA_pol_B_exo1. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 2 hits. SM00306. HintN. 2 hits. SM00486. POLBc. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
TIGRFAMs	TIGR01443. intein_Cterm. 2 hits. TIGR01445. intein_Nterm. 1 hit. TIGR00592. pol2. 1 hit.
PROSITE	PS00116. DNA_POLYMERASE_B. 1 hit. PS50818. INTEIN_C_TER. 2 hits. PS50819. INTEIN_ENDONUCLEASE. 2 hits. PS50817. INTEIN_N_TER. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPOL_METJA

Accession

Primary (citable) accession number: Q58295

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program