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Protein

DNA polymerase

Gene

pol

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase (EC:2.7.7.7)
Cleaved into the following 2 chains:
Gene namesi
Name:pol
Ordered Locus Names:MJ0885
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000073171 – 425DNA polymerase, 1st partSequence analysisAdd BLAST425
ChainiPRO_0000007318426 – 794Mja pol-1 inteinSequence analysisAdd BLAST369
ChainiPRO_0000007319795 – 882DNA polymerase, 2nd partSequence analysisAdd BLAST88
ChainiPRO_0000007320883 – 1358Mja pol-2 inteinSequence analysisAdd BLAST476
ChainiPRO_00000073211359 – 1634DNA polymerase, 3rd partSequence analysisAdd BLAST276

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRIDEiQ58295

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ_0885

Structurei

3D structure databases

ProteinModelPortaliQ58295
SMRiQ58295
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini552 – 693DOD-type homing endonuclease 1PROSITE-ProRule annotationAdd BLAST142
Domaini1163 – 1295DOD-type homing endonuclease 2PROSITE-ProRule annotationAdd BLAST133

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiarCOG00328 Archaea
arCOG00329 Archaea
arCOG03145 Archaea
COG0417 LUCA
InParanoidiQ58295
KOiK02319
OMAiYAHNSYY
OrthoDBiPOG093Z00EA

Family and domain databases

Gene3Di3.10.28.10, 2 hits
3.30.420.10, 1 hit
3.90.1600.10, 3 hits
InterProiView protein in InterPro
IPR006172 DNA-dir_DNA_pol_B
IPR017964 DNA-dir_DNA_pol_B_CS
IPR006133 DNA-dir_DNA_pol_B_exonuc
IPR006134 DNA-dir_DNA_pol_B_multi_dom
IPR023211 DNA_pol_palm_dom_sf
IPR003586 Hint_dom_C
IPR003587 Hint_dom_N
IPR036844 Hint_dom_sf
IPR027434 Homing_endonucl
IPR006142 INTEIN
IPR030934 Intein_C
IPR004042 Intein_endonuc
IPR006141 Intein_N
IPR004860 LAGLIDADG_2
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF00136 DNA_pol_B, 2 hits
PF03104 DNA_pol_B_exo1, 1 hit
PF14528 LAGLIDADG_3, 1 hit
PRINTSiPR00379 INTEIN
SMARTiView protein in SMART
SM00305 HintC, 2 hits
SM00306 HintN, 2 hits
SM00486 POLBc, 1 hit
SUPFAMiSSF51294 SSF51294, 4 hits
SSF53098 SSF53098, 1 hit
SSF55608 SSF55608, 2 hits
TIGRFAMsiTIGR01443 intein_Cterm, 2 hits
TIGR01445 intein_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS00116 DNA_POLYMERASE_B, 1 hit
PS50818 INTEIN_C_TER, 2 hits
PS50819 INTEIN_ENDONUCLEASE, 2 hits
PS50817 INTEIN_N_TER, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGMSMGKIKI DALIDNTYKT IEDKAVIYLY LINSILKDRD FKPYFYVELH
60 70 80 90 100
KEKVENEDIE KIKEFLLKND LLKFVENIEV VKKIILRKEK EVIKIIATHP
110 120 130 140 150
QKVPKLRKIK ECEIVKEIYE HDIPFAKRYL IDNEIIPMTY WDFENKKPVS
160 170 180 190 200
IEIPKLKSVA FDMEVYNRDT EPNPERDPIL MASFWDENGG KVITYKEFNH
210 220 230 240 250
PNIEVVKNEK ELIKKIIETL KEYDVIYTYN GDNFDFPYLK ARAKIYGIDI
260 270 280 290 300
NLGKDGEELK IKRGGMEYRS YIPGRVHIDL YPISRRLLKL TKYTLEDVVY
310 320 330 340 350
NLFGIEKLKI PHTKIVDYWA NNDKTLIEYS LQDAKYTYKI GKYFFPLEVM
360 370 380 390 400
FSRIVNQTPF EITRMSSGQM VEYLLMKRAF KENMIVPNKP DEEEYRRRVL
410 420 430 440 450
TTYEGGYVKE PEKGMFEDII SMDFRCHPKG TKVVVKGKGI VNIEDVKEGN
460 470 480 490 500
YVLGIDGWQK VKKVWKYEYE GELINVNGLK CTPNHKIPLR YKIKHKKINK
510 520 530 540 550
NDYLVRDIYA KSLLTKFKGE GKLILCKDFE TIGNYEKYIN DMDEDFILKS
560 570 580 590 600
ELIGILLAEG HLLRRDIEYF DSSRGKKRIS HQYRVEITVN EDEKDFIEKI
610 620 630 640 650
KYIFKKLFNY ELYVRRKKGT KAITLGCAKK DIYLKIEEIL KNKEKYLPNA
660 670 680 690 700
ILRGFFEGDG YVNTVRRAVV VNQGTNNYDK IKFIASLLDR LGIKYSFYTY
710 720 730 740 750
SYEERGKKLK RYVIEIFSKG DLIKFSILIS FISRRKNNLL NEIIRQKTLY
760 770 780 790 800
KIGDYGFYDL DDVCVSLESY KGEVYDLTLE GRPYYFANGI LTHNSLYPSI
810 820 830 840 850
IISYNISPDT LDCECCKDVS EKILGHWFCK KKEGLIPKTL RNLIERRINI
860 870 880 890 900
KRRMKKMAEI GEINEEYNLL DYEQKSLKIL ANSILPDEYL TIIEEDGIKV
910 920 930 940 950
VKIGEYIDDL MRKHKDKIKF SGISEILETK NLKTFSFDKI TKKCEIKKVK
960 970 980 990 1000
ALIRHPYFGK AYKIKLRSGR TIKVTRGHSL FKYENGKIVE VKGDDVRFGD
1010 1020 1030 1040 1050
LIVVPKKLTC VDKEVVINIP KRLINADEEE IKDLVITKHK DKAFFVKLKK
1060 1070 1080 1090 1100
TLEDIENNKL KVIFDDCILY LKELGLIDYN IIKKINKVDI KILDEEKFKA
1110 1120 1130 1140 1150
YKKYFDTVIE HGNFKKGRCN IQYIKIKDYI ANIPDKEFED CEIGAYSGKI
1160 1170 1180 1190 1200
NALLKLDEKL AKFLGFFVTR GRLKKQKLKG ETVYEISVYK SLPEYQKEIA
1210 1220 1230 1240 1250
ETFKEVFGAG SMVKDKVTMD NKIVYLVLKY IFKCGDKDKK HIPEELFLAS
1260 1270 1280 1290 1300
ESVIKSFLDG FLKAKKNSHK GTSTFMAKDE KYLNQLMILF NLVGIPTRFT
1310 1320 1330 1340 1350
PVKNKGYKLT LNPKYGTVKD LMLDEVKEIE AFEYSGYVYD LSVEDNENFL
1360 1370 1380 1390 1400
VNNIYAHNSV YGYLAFPRAR FYSRECAEIV TYLGRKYILE TVKEAEKFGF
1410 1420 1430 1440 1450
KVLYIDTDGF YAIWKEKISK EELIKKAMEF VEYINSKLPG TMELEFEGYF
1460 1470 1480 1490 1500
KRGIFVTKKR YALIDENGRV TVKGLEFVRR DWSNIAKITQ RRVLEALLVE
1510 1520 1530 1540 1550
GSIEKAKKII QDVIKDLREK KIKKEDLIIY TQLTKDPKEY KTTAPHVEIA
1560 1570 1580 1590 1600
KKLMREGKRI KVGDIIGYII VKGTKSISER AKLPEEVDID DIDVNYYIDN
1610 1620 1630
QILPPVLRIM EAVGVSKNEL KKEGAQLTLD KFFK
Length:1,634
Mass (Da):191,710
Last modified:December 15, 1998 - v2
Checksum:i84A1FAFAB1F97DDD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA Translation: AAB98889.1
PIRiE64410

Genome annotation databases

EnsemblBacteriaiAAB98889; AAB98889; MJ_0885
KEGGimja:MJ_0885

Entry informationi

Entry nameiDPOL_METJA
AccessioniPrimary (citable) accession number: Q58295
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: April 25, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families
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