Reviewed, UniProtKB/Swiss-Prot Q58274 (HDRC2_METJA)

Names and origin

Protein names

Recommended name:
    CoB--CoM heterodisulfide reductase iron-sulfur subunit C 2
    EC=1.8.98.1

Gene names

Name:	hdrC2
Ordered Locus Names:	MJ0864

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B) By similarity.
Catalytic activity	Coenzyme B + coenzyme M + methanophenazine = N-(7-((2-sulfoethyl)dithio)heptanoyl)-O(3)-phospho-L-threonine + dihydromethanophenazine.
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity.
Pathway	Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.
Subunit structure	The heterodisulfide reductase is composed of three subunits; hdrA, hdrB and hdrC By similarity.
Sequence similarities	Belongs to the hdrC family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Methanogenesis
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cofactor metabolic process Inferred from electronic annotation. Source: InterPro methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW CoB--CoM heterodisulfide reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	CoB--CoM heterodisulfide reductase iron-sulfur subunit C 2		PRO_0000150074
Regions
	Domain	26 – 56	31	4Fe-4S ferredoxin-type 1
	Domain	67 – 99	33	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	36	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	39	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	42	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	46	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	79	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	82	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	85	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	89	1	Iron-sulfur 1 (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q58274-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: DCF74D7A6996117A	FASTA	186	21,049
	10 20 30 40 50 60 MAVIKLDEVN KNFVNEVIEA GKLVLGEDIV KSIKACYQCG TCTGSCPSGR RTAYRTRKVL 70 80 90 100 110 120 RKVLLGLDDV LDSDDIWYCT TCYTCYERCP RDVKITEIIK TLRNIAAQKG NMALAHRKTA 130 140 150 160 170 180 SYVLRFGHAV PANNQIVELR GKLGLPAKSP TAQFSEKDLE EVRTLIKELK FDKLIAFDWE KMDLKE

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98869.1.
PIR	H64407.
RefSeq	NP_247859.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1451753.
GenomeReviews	Gene locus MJ0864 in contig L77117_GR.
KEGG	mja:MJ0864.
NMPDR	fig|243232.1.peg.896.
TIGR	MJ0864.
Phylogenomic databases
HOGENOM	Q58274.
OMA	GLNTRRI.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0864-MON.
BRENDA	1.8.98.1. 256362.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR017680. CoB/CoM_hetero-S_Rdtase_csu. IPR012285. Fum_reductase_C. [Graphical view]
Gene3D	G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
TIGRFAMs	TIGR03290. CoB_CoM_SS_C. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HDRC2_METJA

Accession

Primary (citable) accession number: Q58274

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)