Q58261 (MTRA_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetrahydromethanopterin S-methyltransferase subunit A
EC=2.1.1.86

Alternative name(s):
N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit A

Gene names

Name:	mtrA
Ordered Locus Names:	MJ0851

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step By similarity. HAMAP MF_01093
Catalytic activity	5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate = 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate. HAMAP MF_01093
Cofactor	Binds 1 5'-hydroxybenzimidazolyl-cobamide group By similarity.
Pathway	One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2. HAMAP MF_01093
Subunit structure	The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH By similarity.
Subcellular location	Cell membrane; Single-pass membrane protein By similarity HAMAP MF_01093.
Sequence similarities	Belongs to the MtrA family.
Sequence caution	The sequence AAB98856.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Cobalt
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW sodium ion transport Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vesicle membrane Inferred from electronic annotation. Source: InterPro
Molecular function	tetrahydromethanopterin S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Tetrahydromethanopterin S-methyltransferase subunit A HAMAP MF_01093		PRO_0000147503
Regions
	Topological domain	1 – 220	220	Cytoplasmic Potential
	Transmembrane	221 – 241	21	Helical; Potential
Sites
	Binding site	85	1	5'-hydroxybenzimidazolyl-cobamide cofactor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58261 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 155EAE6418E09B8D	FASTA	241	25,880
	10 20 30 40 50 60 MANKREPAPG WPIVSGEYVV GNPESCVGVV TLGSHGLEQA CIDAGAAIAG PCHTENLGIE 70 80 90 100 110 120 KVVANYISNP NIRFMILCGS EVQGHITGQC FKALWENGIG DDGGIIGAKG AIPFLENVNK 130 140 150 160 170 180 EAVERFRRQI VEVVDLIDCE DIGKITQAIK ECLSKDPGAI DEDPFIIELE GGKGGGEEEE 190 200 210 220 230 240 GVIKPITPEM AIIESRMRLI GNEMCYNGLL AKWQAGYYNG KIQGIATGLF LMLLIMGILM F

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98856.1. Different initiation.
PIR	C64406.
RefSeq	NP_247845.1. NC_000909.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1451739.
GenomeReviews	Gene locus MJ0851 in contig L77117_GR.
KEGG	mja:MJ_0851.
NMPDR	fig|243232.1.peg.882.
TIGR	MJ0851.
Phylogenomic databases
HOGENOM	HBG542039.
OMA	EVKGHIT.
ProtClustDB	PRK00964.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0851-MONOMER.
Family and domain databases
HAMAP	MF_01093. MtrA. [Tree]
InterPro	IPR005778. MeTrfase_A. [Graphical view]
KO	K00577.
Pfam	PF04208. MtrA. 1 hit. [Graphical view]
PIRSF	PIRSF009452. MtrA_MtxA. 1 hit.
TIGRFAMs	TIGR01111. MtrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTRA_METJA

Accession

Primary (citable) accession number: Q58261

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 29, 2005
Last modified:	December 14, 2011
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program