Q58258 (MTRD_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetrahydromethanopterin S-methyltransferase subunit D
EC=2.1.1.86

Alternative name(s):
N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit D

Gene names

Name:	mtrD
Ordered Locus Names:	MJ0848

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step By similarity. HAMAP MF_01097
Catalytic activity	5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate = 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate. HAMAP MF_01097
Pathway	One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2. HAMAP MF_01097
Subunit structure	The complex is composed of 8 subunits; mtrA, mtrB, mtrC, mtrD, mtrE, mtrF, mtrG and mtrH By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential HAMAP MF_01097.
Sequence similarities	Belongs to the mtrD family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW sodium ion transport Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vesicle membrane Inferred from electronic annotation. Source: InterPro
Molecular function	tetrahydromethanopterin S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Tetrahydromethanopterin S-methyltransferase subunit D HAMAP MF_01097		PRO_0000147530
Regions
	Transmembrane	7 – 27	21	Helical; Potential
	Transmembrane	60 – 80	21	Helical; Potential
	Transmembrane	88 – 108	21	Helical; Potential
	Transmembrane	142 – 162	21	Helical; Potential
	Transmembrane	168 – 188	21	Helical; Potential
	Transmembrane	210 – 230	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q58258 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: B9D10D88DC6B64F3	FASTA	230	22,879
	10 20 30 40 50 60 MDIVSAIVPL IEMTIAGAII NASVHFIPVG GAPAAMATST GVGTGTTQLA AGAGFTGLMG 70 80 90 100 110 120 AAVMASNVGL SPIGMALIMI SGAVSSMIML GVTMLIGQLI YVFGVGVVPA ADKCEIDPIT 130 140 150 160 170 180 KDPQKPYVTP GTTGHGVPTV CFVSGLIGAA LGGIGGALAY IALRKLGLDP GVAGMLAVGF 190 200 210 220 230 FFINAVLASY NIGGTIEGFH DPKFKKMPNG VIASTVASLL FGIISVLMVL

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98853.1.
PIR	H64405.
RefSeq	NP_247842.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58258.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1451736.
GenomeReviews	Gene locus MJ0848 in contig L77117_GR.
KEGG	mja:MJ_0848.
NMPDR	fig|243232.1.peg.879.
TIGR	MJ0848.
Phylogenomic databases
HOGENOM	HBG551221.
OMA	VHFVPVG.
ProtClustDB	PRK00968.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0848-MONOMER.
Family and domain databases
HAMAP	MF_01097. MtrD. [Tree]
InterPro	IPR005779. MeTrfase_D. [Graphical view]
KO	K00580.
Pfam	PF04207. MtrD. 1 hit. [Graphical view]
PIRSF	PIRSF016552. MtrD. 1 hit.
ProDom	PD013594. MeTrfase_D. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01112. MtrD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTRD_METJA

Accession

Primary (citable) accession number: Q58258

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 16, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program