Methyl-coenzyme M reductase I subunit alpha - Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

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Q58256 (MCRA_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Methyl-coenzyme M reductase I subunit alpha
Short name=MCR I alpha
EC=2.8.4.1

Alternative name(s):
Coenzyme-B sulfoethylthiotransferase alpha

Gene names

Name:	mcrA
Ordered Locus Names:	MJ0846

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide By similarity.
Catalytic activity	Methyl-CoM + CoB = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.
Pathway	One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Subunit structure	Hexamer of two alpha, two beta, and two gamma chains By similarity.

Ontologies

Keywords
Biological process	Methanogenesis
Ligand	Metal-binding Nickel
Molecular function	Transferase
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 553

553

Methyl-coenzyme M reductase I subunit alpha

PRO_0000147452

Sites

Metal binding

150

Nickel By similarity

Amino acid modifications

Modified residue

260

Pros-methylhistidine By similarity

Modified residue

273

5-methylarginine By similarity

Experimental info

Sequence conflict

424

N → F in AAL29289. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q58256 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: CC3AB9AC4F745A24
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FASTA

553

61,266

        10         20         30         40         50         60 
MDAEKRLFLK ALKEKFEEDP REKYTKFYVF GGWRQSARKR EFVEAAQKLI EKRGGIPFYN 

        70         80         90        100        110        120 
PDIGVPLGQR KLMPYKVSNT DAIVEGDDLH FMNNAAMQQF WDDIRRTVIV GMDTAHAVLE 

       130        140        150        160        170        180 
KRLGVEVTPE TINEYMETIN HALPGGAVVQ EHMVEVHPAL VWDCYAKIFT GDDELADEID 

       190        200        210        220        230        240 
KRFLIDINKL FPEEQAEQIK KAIGKRTYQV SRVPTLVGRV CDGGTIARWS AMQIGMSFIT 

       250        260        270        280        290        300 
AYKLCAGEAA IADFSYAAKH ADVIQMASFL PARRARGPNE PGGIFFGVLA DIVQTSRVSD 

       310        320        330        340        350        360 
DPVEQSLEVV AAGAMLYDQI WLGGYMSGGV GFTQYATATY TDDILDDFSY YGYDYITKKY 

       370        380        390        400        410        420 
GGCNSVKPTM DVVEDIATEV TLYGLEQYDT FPALLEDHFG GSQRAGVTAA AAGITTALAT 

       430        440        450        460        470        480 
GNSNAGVNGW YLSQILHKEY HSRLGFYGYD LQDQCGAANS LSFRNDEGSP LELRGPNYPN 

       490        500        510        520        530        540 
YAMNVGHQGE YAGITQAAHS ARGDAFALNP LIKVAFADPS LVFDFTHPRK EFARGALREF 

       550 
EPAGERDPII PAH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"The mcrA gene as an alternative to 16S rRNA in the phylogenetic analysis of methanogen populations in landfill." Luton P.E., Wayne J.M., Sharp R.J., Riley P.W. Microbiology 148:3521-3530(2002) [PubMed: 12427943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 340-478. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98851.1. AF414040 Genomic DNA. Translation: AAL29289.1.
RefSeq	NP_247840.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58256.
SMR	Q58256. Positions 7-552.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1451734.
GenomeReviews	Gene locus MJ0846 in contig L77117_GR.
KEGG	mja:MJ_0846.
NMPDR	fig\|243232.1.peg.877.
TIGR	MJ0846.
Phylogenomic databases
HOGENOM	HBG541009.
OMA	TMDVVED.
ProtClustDB	CLSK876061.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0846-MONOMER.
Family and domain databases
InterPro	IPR022681. MCR_a/b_chain_a-bundle. IPR016212. Me_CoM_Rdtase_asu. IPR008924. Me_CoM_Rdtase_asu/bsu_C. IPR009047. Me_CoM_Rdtase_asu_C. IPR003183. Me_CoM_Rdtase_asu_N. IPR015811. Me_CoM_Rdtase_asu_N_sub1. IPR015823. Me_CoM_Rdtase_asu_N_sub2. IPR009024. Me_CoM_Rdtase_Fd-like_fold. [Graphical view]
Gene3D	G3DSA:1.20.840.10. MCR_a/b_chain_a-bundle. 1 hit. G3DSA:3.90.390.10. Me_CoM_Rdtase_asu_N_sub1. 1 hit. G3DSA:3.30.70.470. Me_CoM_Rdtase_asu_N_sub2. 1 hit.
KO	K00399.
Pfam	PF02249. MCR_alpha. 1 hit. PF02745. MCR_alpha_N. 1 hit. [Graphical view]
PIRSF	PIRSF000262. MCR_alpha. 1 hit.
SUPFAM	SSF48081. MCR_alpha_beta_C. 1 hit. SSF55088. MCR_fer_like. 1 hit.
TIGRFAMs	TIGR03256. Met_CoM_red_alp. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCRA_METJA

Accession

Primary (citable) accession number: Q58256
Secondary accession number(s): Q977G8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	January 25, 2012
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents