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Q58256 (MCRA_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methyl-coenzyme M reductase I subunit alpha

Short name=MCR I alpha
EC=2.8.4.1
Alternative name(s):
Coenzyme-B sulfoethylthiotransferase alpha
Gene names
Name:mcrA
Ordered Locus Names:MJ0846
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus

Protein attributes

Sequence length553 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide By similarity.

Catalytic activity

Methyl-CoM + CoB = CoM-S-S-CoB + methane.

Cofactor

Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.

Pathway

One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.

Subunit structure

Hexamer of two alpha, two beta, and two gamma chains By similarity.

Ontologies

Keywords
   Biological processMethanogenesis
   LigandMetal-binding
Nickel
   Molecular functionTransferase
   PTMMethylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmethanogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncoenzyme-B sulfoethylthiotransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 553553Methyl-coenzyme M reductase I subunit alpha
PRO_0000147452

Sites

Metal binding1501Nickel By similarity

Amino acid modifications

Modified residue2601Pros-methylhistidine By similarity
Modified residue27315-methylarginine By similarity

Experimental info

Sequence conflict4241N → F in AAL29289. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q58256 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: CC3AB9AC4F745A24

FASTA55361,266
        10         20         30         40         50         60 
MDAEKRLFLK ALKEKFEEDP REKYTKFYVF GGWRQSARKR EFVEAAQKLI EKRGGIPFYN 

        70         80         90        100        110        120 
PDIGVPLGQR KLMPYKVSNT DAIVEGDDLH FMNNAAMQQF WDDIRRTVIV GMDTAHAVLE 

       130        140        150        160        170        180 
KRLGVEVTPE TINEYMETIN HALPGGAVVQ EHMVEVHPAL VWDCYAKIFT GDDELADEID 

       190        200        210        220        230        240 
KRFLIDINKL FPEEQAEQIK KAIGKRTYQV SRVPTLVGRV CDGGTIARWS AMQIGMSFIT 

       250        260        270        280        290        300 
AYKLCAGEAA IADFSYAAKH ADVIQMASFL PARRARGPNE PGGIFFGVLA DIVQTSRVSD 

       310        320        330        340        350        360 
DPVEQSLEVV AAGAMLYDQI WLGGYMSGGV GFTQYATATY TDDILDDFSY YGYDYITKKY 

       370        380        390        400        410        420 
GGCNSVKPTM DVVEDIATEV TLYGLEQYDT FPALLEDHFG GSQRAGVTAA AAGITTALAT 

       430        440        450        460        470        480 
GNSNAGVNGW YLSQILHKEY HSRLGFYGYD LQDQCGAANS LSFRNDEGSP LELRGPNYPN 

       490        500        510        520        530        540 
YAMNVGHQGE YAGITQAAHS ARGDAFALNP LIKVAFADPS LVFDFTHPRK EFARGALREF 

       550 
EPAGERDPII PAH 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L77117 Genomic DNA. Translation: AAB98851.1.
AF414040 Genomic DNA. Translation: AAL29289.1.
RefSeqNP_247840.1. NC_000909.1.

3D structure databases

ProteinModelPortalQ58256.
SMRQ58256. Positions 7-552.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243232.MJ0846.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB98851; AAB98851; MJ_0846.
GeneID1451734.
KEGGmja:MJ_0846.

Phylogenomic databases

eggNOGCOG4058.
KOK00399.
OMAAHSARGD.
PhylomeDBQ58256.

Enzyme and pathway databases

UniPathwayUPA00646; UER00699.

Family and domain databases

Gene3D1.20.840.10. 1 hit.
3.30.70.470. 1 hit.
3.90.390.10. 1 hit.
InterProIPR022681. MCR_a/b_chain_a-bundle.
IPR016212. Me_CoM_Rdtase_asu.
IPR008924. Me_CoM_Rdtase_asu/bsu_C.
IPR009047. Me_CoM_Rdtase_asu_C.
IPR003183. Me_CoM_Rdtase_asu_N.
IPR015811. Me_CoM_Rdtase_asu_N_sub1.
IPR015823. Me_CoM_Rdtase_asu_N_sub2.
IPR009024. Me_CoM_Rdtase_Fd-like_fold.
[Graphical view]
PfamPF02249. MCR_alpha. 1 hit.
PF02745. MCR_alpha_N. 1 hit.
[Graphical view]
PIRSFPIRSF000262. MCR_alpha. 1 hit.
SUPFAMSSF48081. SSF48081. 1 hit.
SSF55088. SSF55088. 1 hit.
TIGRFAMsTIGR03256. met_CoM_red_alp. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMCRA_METJA
AccessionPrimary (citable) accession number: Q58256
Secondary accession number(s): Q977G8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: July 9, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names