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Q58256

- MCRA_METJA

UniProt

Q58256 - MCRA_METJA

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Protein

Methyl-coenzyme M reductase I subunit alpha

Gene

mcrA

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide.By similarity

Catalytic activityi

Methyl-CoM + CoB = CoM-S-S-CoB + methane.

Cofactori

coenzyme F430By similarityNote: Binds 1 coenzyme F430 noncovalently per subunit. Coenzyme F430 is a yellow nickel porphinoid.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi150 – 1501NickelBy similarity

GO - Molecular functioni

  1. coenzyme-B sulfoethylthiotransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. methanogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Methanogenesis

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00646; UER00699.

Names & Taxonomyi

Protein namesi
Recommended name:
Methyl-coenzyme M reductase I subunit alpha (EC:2.8.4.1)
Short name:
MCR I alpha
Alternative name(s):
Coenzyme-B sulfoethylthiotransferase alpha
Gene namesi
Name:mcrA
Ordered Locus Names:MJ0846
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 553553Methyl-coenzyme M reductase I subunit alphaPRO_0000147452Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei260 – 2601Pros-methylhistidineBy similarity
Modified residuei274 – 27415-methylarginineBy similarity

Keywords - PTMi

Methylation

Interactioni

Subunit structurei

Hexamer of two alpha, two beta, and two gamma chains.By similarity

Protein-protein interaction databases

STRINGi243232.MJ0846.

Structurei

3D structure databases

ProteinModelPortaliQ58256.
SMRiQ58256. Positions 7-552.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiCOG4058.
InParanoidiQ58256.
KOiK00399.
OMAiAHSARGD.
PhylomeDBiQ58256.

Family and domain databases

Gene3Di1.20.840.10. 1 hit.
3.30.70.470. 1 hit.
3.90.390.10. 1 hit.
InterProiIPR022681. MCR_a/b_chain_a-bundle.
IPR016212. Me_CoM_Rdtase_asu.
IPR008924. Me_CoM_Rdtase_asu/bsu_C.
IPR009047. Me_CoM_Rdtase_asu_C.
IPR003183. Me_CoM_Rdtase_asu_N.
IPR015811. Me_CoM_Rdtase_asu_N_sub1.
IPR015823. Me_CoM_Rdtase_asu_N_sub2.
IPR009024. Me_CoM_Rdtase_Fd-like_fold.
[Graphical view]
PfamiPF02249. MCR_alpha. 1 hit.
PF02745. MCR_alpha_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000262. MCR_alpha. 1 hit.
SUPFAMiSSF48081. SSF48081. 1 hit.
SSF55088. SSF55088. 1 hit.
TIGRFAMsiTIGR03256. met_CoM_red_alp. 1 hit.

Sequencei

Sequence statusi: Complete.

Q58256-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDAEKRLFLK ALKEKFEEDP REKYTKFYVF GGWRQSARKR EFVEAAQKLI
60 70 80 90 100
EKRGGIPFYN PDIGVPLGQR KLMPYKVSNT DAIVEGDDLH FMNNAAMQQF
110 120 130 140 150
WDDIRRTVIV GMDTAHAVLE KRLGVEVTPE TINEYMETIN HALPGGAVVQ
160 170 180 190 200
EHMVEVHPAL VWDCYAKIFT GDDELADEID KRFLIDINKL FPEEQAEQIK
210 220 230 240 250
KAIGKRTYQV SRVPTLVGRV CDGGTIARWS AMQIGMSFIT AYKLCAGEAA
260 270 280 290 300
IADFSYAAKH ADVIQMASFL PARRARGPNE PGGIFFGVLA DIVQTSRVSD
310 320 330 340 350
DPVEQSLEVV AAGAMLYDQI WLGGYMSGGV GFTQYATATY TDDILDDFSY
360 370 380 390 400
YGYDYITKKY GGCNSVKPTM DVVEDIATEV TLYGLEQYDT FPALLEDHFG
410 420 430 440 450
GSQRAGVTAA AAGITTALAT GNSNAGVNGW YLSQILHKEY HSRLGFYGYD
460 470 480 490 500
LQDQCGAANS LSFRNDEGSP LELRGPNYPN YAMNVGHQGE YAGITQAAHS
510 520 530 540 550
ARGDAFALNP LIKVAFADPS LVFDFTHPRK EFARGALREF EPAGERDPII

PAH
Length:553
Mass (Da):61,266
Last modified:December 15, 1998 - v2
Checksum:iCC3AB9AC4F745A24
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti424 – 4241N → F in AAL29289. (PubMed:12427943)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98851.1.
AF414040 Genomic DNA. Translation: AAL29289.1.
RefSeqiNP_247840.1. NC_000909.1.
WP_010870360.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98851; AAB98851; MJ_0846.
GeneIDi1451734.
KEGGimja:MJ_0846.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98851.1 .
AF414040 Genomic DNA. Translation: AAL29289.1 .
RefSeqi NP_247840.1. NC_000909.1.
WP_010870360.1. NC_000909.1.

3D structure databases

ProteinModelPortali Q58256.
SMRi Q58256. Positions 7-552.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJ0846.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB98851 ; AAB98851 ; MJ_0846 .
GeneIDi 1451734.
KEGGi mja:MJ_0846.

Phylogenomic databases

eggNOGi COG4058.
InParanoidi Q58256.
KOi K00399.
OMAi AHSARGD.
PhylomeDBi Q58256.

Enzyme and pathway databases

UniPathwayi UPA00646 ; UER00699 .

Family and domain databases

Gene3Di 1.20.840.10. 1 hit.
3.30.70.470. 1 hit.
3.90.390.10. 1 hit.
InterProi IPR022681. MCR_a/b_chain_a-bundle.
IPR016212. Me_CoM_Rdtase_asu.
IPR008924. Me_CoM_Rdtase_asu/bsu_C.
IPR009047. Me_CoM_Rdtase_asu_C.
IPR003183. Me_CoM_Rdtase_asu_N.
IPR015811. Me_CoM_Rdtase_asu_N_sub1.
IPR015823. Me_CoM_Rdtase_asu_N_sub2.
IPR009024. Me_CoM_Rdtase_Fd-like_fold.
[Graphical view ]
Pfami PF02249. MCR_alpha. 1 hit.
PF02745. MCR_alpha_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000262. MCR_alpha. 1 hit.
SUPFAMi SSF48081. SSF48081. 1 hit.
SSF55088. SSF55088. 1 hit.
TIGRFAMsi TIGR03256. met_CoM_red_alp. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  2. "The mcrA gene as an alternative to 16S rRNA in the phylogenetic analysis of methanogen populations in landfill."
    Luton P.E., Wayne J.M., Sharp R.J., Riley P.W.
    Microbiology 148:3521-3530(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 340-478.
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiMCRA_METJA
AccessioniPrimary (citable) accession number: Q58256
Secondary accession number(s): Q977G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: November 26, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3