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Q58256

- MCRA_METJA

UniProt

Q58256 - MCRA_METJA

Protein

Methyl-coenzyme M reductase I subunit alpha

Gene

mcrA

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide.By similarity

    Catalytic activityi

    Methyl-CoM + CoB = CoM-S-S-CoB + methane.

    Cofactori

    Binds 1 coenzyme F430 noncovalently per subunit. Coenzyme F430 is a yellow nickel porphinoid By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi150 – 1501NickelBy similarity

    GO - Molecular functioni

    1. coenzyme-B sulfoethylthiotransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. methanogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Methanogenesis

    Keywords - Ligandi

    Metal-binding, Nickel

    Enzyme and pathway databases

    UniPathwayiUPA00646; UER00699.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methyl-coenzyme M reductase I subunit alpha (EC:2.8.4.1)
    Short name:
    MCR I alpha
    Alternative name(s):
    Coenzyme-B sulfoethylthiotransferase alpha
    Gene namesi
    Name:mcrA
    Ordered Locus Names:MJ0846
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 553553Methyl-coenzyme M reductase I subunit alphaPRO_0000147452Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei260 – 2601Pros-methylhistidineBy similarity
    Modified residuei274 – 27415-methylarginineBy similarity

    Keywords - PTMi

    Methylation

    Interactioni

    Subunit structurei

    Hexamer of two alpha, two beta, and two gamma chains.By similarity

    Protein-protein interaction databases

    STRINGi243232.MJ0846.

    Structurei

    3D structure databases

    ProteinModelPortaliQ58256.
    SMRiQ58256. Positions 7-552.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG4058.
    KOiK00399.
    OMAiAHSARGD.
    PhylomeDBiQ58256.

    Family and domain databases

    Gene3Di1.20.840.10. 1 hit.
    3.30.70.470. 1 hit.
    3.90.390.10. 1 hit.
    InterProiIPR022681. MCR_a/b_chain_a-bundle.
    IPR016212. Me_CoM_Rdtase_asu.
    IPR008924. Me_CoM_Rdtase_asu/bsu_C.
    IPR009047. Me_CoM_Rdtase_asu_C.
    IPR003183. Me_CoM_Rdtase_asu_N.
    IPR015811. Me_CoM_Rdtase_asu_N_sub1.
    IPR015823. Me_CoM_Rdtase_asu_N_sub2.
    IPR009024. Me_CoM_Rdtase_Fd-like_fold.
    [Graphical view]
    PfamiPF02249. MCR_alpha. 1 hit.
    PF02745. MCR_alpha_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000262. MCR_alpha. 1 hit.
    SUPFAMiSSF48081. SSF48081. 1 hit.
    SSF55088. SSF55088. 1 hit.
    TIGRFAMsiTIGR03256. met_CoM_red_alp. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q58256-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDAEKRLFLK ALKEKFEEDP REKYTKFYVF GGWRQSARKR EFVEAAQKLI    50
    EKRGGIPFYN PDIGVPLGQR KLMPYKVSNT DAIVEGDDLH FMNNAAMQQF 100
    WDDIRRTVIV GMDTAHAVLE KRLGVEVTPE TINEYMETIN HALPGGAVVQ 150
    EHMVEVHPAL VWDCYAKIFT GDDELADEID KRFLIDINKL FPEEQAEQIK 200
    KAIGKRTYQV SRVPTLVGRV CDGGTIARWS AMQIGMSFIT AYKLCAGEAA 250
    IADFSYAAKH ADVIQMASFL PARRARGPNE PGGIFFGVLA DIVQTSRVSD 300
    DPVEQSLEVV AAGAMLYDQI WLGGYMSGGV GFTQYATATY TDDILDDFSY 350
    YGYDYITKKY GGCNSVKPTM DVVEDIATEV TLYGLEQYDT FPALLEDHFG 400
    GSQRAGVTAA AAGITTALAT GNSNAGVNGW YLSQILHKEY HSRLGFYGYD 450
    LQDQCGAANS LSFRNDEGSP LELRGPNYPN YAMNVGHQGE YAGITQAAHS 500
    ARGDAFALNP LIKVAFADPS LVFDFTHPRK EFARGALREF EPAGERDPII 550
    PAH 553
    Length:553
    Mass (Da):61,266
    Last modified:December 15, 1998 - v2
    Checksum:iCC3AB9AC4F745A24
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti424 – 4241N → F in AAL29289. (PubMed:12427943)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB98851.1.
    AF414040 Genomic DNA. Translation: AAL29289.1.
    RefSeqiNP_247840.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB98851; AAB98851; MJ_0846.
    GeneIDi1451734.
    KEGGimja:MJ_0846.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB98851.1 .
    AF414040 Genomic DNA. Translation: AAL29289.1 .
    RefSeqi NP_247840.1. NC_000909.1.

    3D structure databases

    ProteinModelPortali Q58256.
    SMRi Q58256. Positions 7-552.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ0846.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB98851 ; AAB98851 ; MJ_0846 .
    GeneIDi 1451734.
    KEGGi mja:MJ_0846.

    Phylogenomic databases

    eggNOGi COG4058.
    KOi K00399.
    OMAi AHSARGD.
    PhylomeDBi Q58256.

    Enzyme and pathway databases

    UniPathwayi UPA00646 ; UER00699 .

    Family and domain databases

    Gene3Di 1.20.840.10. 1 hit.
    3.30.70.470. 1 hit.
    3.90.390.10. 1 hit.
    InterProi IPR022681. MCR_a/b_chain_a-bundle.
    IPR016212. Me_CoM_Rdtase_asu.
    IPR008924. Me_CoM_Rdtase_asu/bsu_C.
    IPR009047. Me_CoM_Rdtase_asu_C.
    IPR003183. Me_CoM_Rdtase_asu_N.
    IPR015811. Me_CoM_Rdtase_asu_N_sub1.
    IPR015823. Me_CoM_Rdtase_asu_N_sub2.
    IPR009024. Me_CoM_Rdtase_Fd-like_fold.
    [Graphical view ]
    Pfami PF02249. MCR_alpha. 1 hit.
    PF02745. MCR_alpha_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000262. MCR_alpha. 1 hit.
    SUPFAMi SSF48081. SSF48081. 1 hit.
    SSF55088. SSF55088. 1 hit.
    TIGRFAMsi TIGR03256. met_CoM_red_alp. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
    2. "The mcrA gene as an alternative to 16S rRNA in the phylogenetic analysis of methanogen populations in landfill."
      Luton P.E., Wayne J.M., Sharp R.J., Riley P.W.
      Microbiology 148:3521-3530(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 340-478.
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

    Entry informationi

    Entry nameiMCRA_METJA
    AccessioniPrimary (citable) accession number: Q58256
    Secondary accession number(s): Q977G8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways

    External Data

    Dasty 3